Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Jpn J Infect Dis ; 68(5): 434-7, 2015.
Article in English | MEDLINE | ID: mdl-25971318

ABSTRACT

We surveyed Rickettsiales bacteria, including Rickettsia, Ehrlichia, Anaplasma, and Neoehrlichia, in wild sika deer (Cervus nippon nippon) from Shizuoka prefecture, Japan. In spleen samples from 187 deer, Anaplasma phagocytophilum (deer type), A. bovis, and A. centrale were successfully detected by PCR assay targeting to 16S rDNA or p44/msp2, and their positive rates were 96.3% (180/187), 53.5% (100/187), and 78.1% (146/187), respectively. Additionally, 2 or 3 Anaplasma species could be detected from a single deer in 165 spleen samples (88.2%), indicating dual or triple infection. In contrast, A. phagocytophilum (human type) 16S rDNA, Rickettsia gltA, Ehrlichia p28/omp-1, and Neoehrlichia 16S rDNA could not be amplified. The serological test of 105 deer serum samples by immunofluorescence assay showed that the detection of antibodies against antigens of A. phagocytophilum HZ (US-human isolate) and Rickettsia japonica YH were 29.5% (31/105) and 75.2% (79/105), respectively. These findings suggest that A. phagocytophilum (deer type), A. centrale, and A. bovis are highly dominant and prevalent in wild sika deer from Shizuoka, a central region of Japan, and that the antibodies against some Rickettsiales bacteria have also been retained in deer blood.


Subject(s)
Anaplasmataceae , Deer/microbiology , Rickettsia , Anaplasmataceae/genetics , Anaplasmataceae/immunology , Anaplasmataceae Infections/microbiology , Anaplasmataceae Infections/veterinary , Animals , Japan , Prevalence , Rickettsia/genetics , Rickettsia/immunology , Rickettsia Infections/microbiology , Rickettsia Infections/veterinary
2.
Jpn J Infect Dis ; 65(1): 79-83, 2012.
Article in English | MEDLINE | ID: mdl-22274164

ABSTRACT

Anaplasma phagocytophilum is an obligate intracellular bacterium and causes a febrile illness in humans and livestock. In nature, this bacterium is sustained in a tick-mammal cycle. Several p44/msp2-related genes are expressed from a single expression locus by gene conversion. In this study, we obtained 119 cDNA sequences of p44/msp2 transcripts from A. phagocytophilum in 6 Haemaphysalis ticks and 3 wild sika deer (Cervus nippon) in Japan. These 119 sequences were classified into 36 different variant sequences based on their similarities. The 36 cDNA sequences were phylogenetically grouped into 2 major clusters--tick- and deer-associated. The tick-associated sequences were further classified into 4 distinct subclusters, suggesting that A. phagocytophilum in ticks seems to selectively express specific p44/msp2 transcripts, such as the transcripts in the 4 subclusters that were closely related to previously identified p44/msp2 genes. The deer-associated sequences were also grouped into 4 subclusters, but these transcripts were probably more diverse than the transcripts derived from ticks. This might be due to the relatively nonselective expression of p44/msp2 in deer or the strain differences in A. phagocytophilum from ticks and deer in separate geographic regions or both. Thus, this study may contribute to the understanding of A. phagocytophilum p44/msp2 expression in nature in Japan.


Subject(s)
Anaplasma phagocytophilum/genetics , Bacterial Outer Membrane Proteins/genetics , Deer/microbiology , Ixodes/microbiology , Anaplasma phagocytophilum/classification , Anaplasma phagocytophilum/isolation & purification , Animals , Cluster Analysis , DNA, Complementary/analysis , DNA, Complementary/genetics , Gene Expression Regulation, Bacterial , Genes, Bacterial , Genetic Variation , Japan/epidemiology , Phylogeny , RNA, Bacterial/genetics , Salivary Glands/microbiology , Sequence Analysis, DNA , Species Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...