Chlorophyll-a specific volume scattering function of phytoplankton.

Chlorophyll-a specific light volume scattering functions (VSFs) by cultured phytoplankton in visible spectrum range is presented. Chlorophyll-a specific VSFs were determined based on the linear least squares method using a measured VSFs with different chlorophyll-a concentrations. We found obvious variability of it in terms of spectral and angular shapes of VSF between cultures. It was also presented that chlorophyll-a specific scattering significantly affected on spectral variation of the remote sensing reflectance, depending on spectral shape of b. This result is useful for developing an advance algorithm of ocean color remote sensing and for deep understanding of light in the sea.

Accurate estimation of the backscattering coefficient by light scattering at two backward angles.

Backscattering coefficients are frequently estimated from light scattering at one backward angle multiplied by a conversion factor. We determined that the shapes of the volume scattering functions (VSFs), particularly for scattering angles larger than 170°, cause significant variations in the conversion factor at 120°. Our approach uses the ratio of scattering at 170° and at 120°, which is a good indicator of the shape differences of the VSFs for most oceanic waters and wavelengths in the visible range. The proposed method provides significant accuracy improvement in the determination of the backscattering coefficients with a prediction error of 3% of the mean.

A new approach to measure the volume scattering function.

We present a novel optical approach to measure the volume scattering function (VSF) by image detection. The instrument design, based upon a combination of two reflectors, uses a unique measurement principle and allows the rapid simultaneous determination of scattering at a wide range of angles. The advantages of the newly developed scattering meter are that: 1) it can determine the scattering function from 8° to 172° at 1° intervals without changing the sensitivity of the detector, without moving any optical parts, and can do so within a few seconds, 2) the unique optical design facilitates determination of the spectral VSF over the full visible spectrum, i.e. it can obtain the VSF at a specific wavelength with an optional wavelength-resolution. Measurements under controlled conditions for the assessment of the instrument agreed well with theoretically predicted scattering functions. Measurements with cultured phytoplankton of different species revealed a significant variety of the VSF together with spectral variation. The observed results will stimulate and improve radiative transfer and/or two-flow models of light in the ocean, which is an important role for ocean color remote sensing algorithm development, particularly for coastal regions.

Simultaneous illumination method: application in a two-channel emission fluorometer with multi-wavelength excitation.

We developed a new illumination method called the simultaneous illumination method. This method does not require synchronization between light sources and sensor signals, which drastically simplifies the instrumentation. As a proof-of-concept, we applied this method to an oceanographic fluorometer. In principle, using this method, one can easily increase the number of characterized emission wavelengths by mounting optical sensors for as many emission wavelengths as needed. Our fluorometer has two emission-wavelength channels and twelve excitation wavelengths. The aim of this prototype is to demonstrate a viable in situ N-channel emission fluorometer with multiple wavelengths of excitation, which has not been previously realized.

Determination of velocity of self-mobile phytoplankton using a self-mixing thin-slice solid-state laser.

We present an analysis of the shoulder-shaped power spectrum observed in the modulated laser output due to feedback light scattered from dynamic changes in self-mobile phytoplankton with flagella in seawater performed using a self-mixing laser Doppler vibrometry system. The power spectrum occasionally has shoulder-shaped broad frequency components superimposed on a Lorentz-type spectrum. This reflects the translational motion of phytoplankton moving across the beam-focus area. The velocity of phytoplankton in the focus area can be obtained by applying a curve fitting procedure to the power spectrum. Moreover, the average velocity and the velocity distribution of phytoplankton can be determined from curve fitting of the long-term power spectrum.

Quick and easy measurement of particle size of Brownian particles and planktons in water using a self-mixing laser.

We describe a method for quickly and easily measuring the size of small particles in suspensions. This method uses a self-mixing laser Doppler measurement with a laser-diode-pumped, thin-slice LiNdP(4)O(12) laser with extremely high optical sensitivity. The average size of the particles in Brownian motion is determined by a Lorentz fitting of the measured power spectrum of the modulated self-mixing laser light resulting from the motion. The dependence of the measured power spectra on particle size and concentration was quantitatively identified from the results of a systematic investigation of small polystyrene latex particles with different diameters and concentrations. The sizes and ratios of particles with different diameters mixed in water were accurately measured. An application of this self-mixing laser method for estimation of the average size of plankton in seawater showed that it is a practical method for characterizing biological species.

Efficient use of an improved radiative transfer code to simulate near-global distributions of satellite-measured radiances.

Two new extension modules that give the water-leaving radiance from the ocean and the snow bidirectional reflectance distribution function were implemented in the latest radiative transfer code. In addition, to simulate the near-global distributions of satellite-measured radiances by using the improved radiative transfer code, we tested and applied the look-up table method together with the process-separation technique of the radiative transfer calculation. The computing time was reduced from 1 year to 20 s to simulate one channel, one scene of the Global Imager image by use of an Alpha 21164A-2 (600-MHz) machine. The error analyses showed that the radiances were simulated with less than 1% error for the nonabsorbing visible channels and approximately 2% error for absorbing channels by use of this method.