Immunotactoid Glomerulopathy and Cryoglobulinemic Nephropathy: Two Entities with Many Similarities. A Unified Conceptual Approach.

Immunotactoid glomerulopathy is a rare disorder that has been characterized at the ultrastructural level. Due to its rarity, there are few comprehensive studies relating to this disorder. Electron microscopy essentially characterizes this disease. The glomerular electron dense deposits which are typical of this condition consist of aggregates of highly organized microtubular structures of various diameters, but generally measuring 30-50 nm in width with a propensity to dispose themselves in parallel bundles intersecting in different planes. This study compares a large series of patients with cryoglobulinemic nephropathy with a series of patients with immunotactoid glomerulopathy to address whether there may be similarities that warrant considering these two entities part of a spectrum. This study reviews the clinicopathologic features of both entities and emphasizes ultrastructural findings that characterize them. Significant immunomorphologic overlap was found when these two disorders were compared in this study. There were also striking similarities in clinical presentation/behavior, laboratory findings and prognosis. Proteomic analysis has also demonstrated similar spectra for both entities. We postulate that immunotactoid glomerulopathy and cryoglobulinemic nephropathy are part of the spectrum of renal manifestations in patients with circulating cryoglobulins and renal disease.

The many faces of cryoglobulinemic nephropathy: a clinico-pathologic study of 47 cases with emphasis on the value of electron microscopy.

The clinical and pathologic manifestations of cryoglobulinemic nephropathy (CN) are heterogeneous. The role that electron microscopy plays in the diagnosis of CN has not been properly evaluated. The main objective of this study was to define the value of ultrastructural evaluation in the diagnosis of CN. Although most of the CN cases in this series exhibited glomerular pathology with a membranoproliferative pattern, a significant number of the cases showed less well-defined morphologic patterns on light microscopic examination (i.e. mesangial proliferation, hyaline thrombi in glomerular capillaries without significant cellular proliferation, exudative glomerulonephritis, etc). Immunofluorescence microscopy also revealed variable immunoglobulin and complement component patterns, some with "full-house" expression of immunoreactants. A subset of these CN cases was associated with light chain restriction. Thus, differential diagnosis can be a challenge as many other glomerulonephritis overlap in immunomorphologic characteristics. Because the immunomorphologic manifestations of CN are so varied, confirmation of a suspected diagnosis of CN or making a diagnosis in a less than a typical immunomorphologic setting required careful ultrastructural evaluation to find unequivocal diagnostic findings or at least supportive evidence in the form of detection of substructure in the electron dense glomerular deposits consistent with cryoglobulins. Even in cases where the light and immunofluorescence findings in suspicious clinical situations were quite consistent with CN, electron microscopy provided the final proof to substantiate the diagnosis in the great majority of the cases.

Further exploration during open appendicectomy; assessment of some common intraoperative findings.

OBJECTIVE: Very few studies are available to relate the final histology of excised appendix with the detailed intra-operative findings during appendectomy, both open and laparoscopic. This study was aimed to correlate the histological features of appendix specimen with the intra operative findings at open appendicectomy (OA) in a bid to determine when to change the planned procedure to include further exploration. Methods : A prospective study that observes the condition of the greater omentum (GO), the vermiform appendix and peritoneal exudates at all OA done for uncomplicated appendicitis. Histological examination of the appendices done using the H&E stain. Results : Eighty-five patients had emergency open OA, their(') ages range from 6 to 62 yrs (median = 23yrs). Histology showed 7 normal appendix (HNA), 56 acute (HAA) and 22 "non acute" appendicitis (HNAA). Negative appendicectomy rate was 8.2%. The GO was sighted more in patients with HAA than HNAA (p=0.00015) and also significantly more inflamed in the former (p=0.00028). It is not significantly inflamed in those with HNAA (p=0.945). The negative predictive value (NPV) of absent GO is 35.7% while the positive predictive value (PPV) of sighted normal GO and inflamed GO are 92.8% and 100% respectively. The PPV and NPV of presence of pus for diseased appendix are 95.8% and 9.8% respectively while those of excess fluid are 94.8% and 10.8%. The PPV and NPV of macroscopic assessment of the appendix for inflammation are 97% and 45.5% respectively giving the diagnostic accuracy of 90.6%. A significant trend of increasing probability of histologically inflamed appendix with increasing severity of macroscopic feature was seen (X(2) = 004 df=1, p<0.005). CONCLUSION: High positive and low negative predictive values are similar for all the three parameters assessed. The macroscopic appearance of the appendix has a predictive likelihood ratio for further exploration.

Ultrasound-guided wire localization of lesions detected on screening mammography in Osogbo, Nigeria and its impact on breast conservative surgery.

BACKGROUND: Wire localization for planned surgical treatment in the management of breast cancer is underutilized in our environment. The objective of this study is to assess the role of ultrasound-guided wire localization of breast masses detected on screening mammography and its impact on biopsy and breast conservative surgery in our environment. MATERIALS AND METHODS: A prospective study of 189 women who presented for screening mammography following a health campaign on breast cancer within a six-month period. Wire localization for mammographic detected lesions was done under ultrasound guidance. The lesions excised were sent for specimen radiography and histology. RESULTS: Ten women had wire localization of breast lesions and subsequent excision. Three lesions were on the right and 7 on the left; out of which 3 were malignant. The mean volume of excised tissue was 74.27 ± 30.16 cm(3). CONCLUSION: Early detection of breast cancer is possible and practicable in our environment. Wire localization of detectable lesions on mammography will assist in better surgical management and improve prognosis.