ABSTRACT
The chemical constituents from the flower, leaf and stem of Helichrysum armenium DC. (Asteraceae) growing in Iran were obtained by hydrodistillation and analyzed by GC and GC/MS. The oil of flower was characterized by higher amount of limonene (21.2%), alpha-cadinol (18.2%), borneol (11.9%), delta-cadinene (9.0%), bornyl acetate (8.0%) and alpha-humulene (7.3%). Twenty one constituents representing 96.2% of the chromatographical leaf oil were identified of which limonene (29.2%), alpha-pinene (14.4%), caryophyllene oxide (6.5%), alpha-gurjunene (6.3%), bornyl acetate (5.5%) and torreyol (5.2%) were the major components. The main components of the stem oil were limonene (23.6%), alpha-pinene (13.4%), spathulenol (6.4%), alpha-gurjunene (6.3%), caryophyllene oxide (5.3%), bornyl acetate (5.2%), beta-cubebene (4.8%) and delta-cadinene (4.3%). The composition of the oils is different, although the most abundant components are identical in leaf oil (96.2%). The antimicrobial effect of flower, leaf and stem essential oils from Helichrysum armenium was studied according to the agar diffusion cup method. The essential oils had a moderate effect on the Gram-positive and Gram-negative bacteria and had a substantial fungicidal effect on the fungi under study.
Subject(s)
Anti-Infective Agents/pharmacology , Asteraceae/chemistry , Oils, Volatile/pharmacology , Flowers/chemistry , Gas Chromatography-Mass Spectrometry , Oils, Volatile/analysis , Plant Leaves/chemistry , Plant Stems/chemistryABSTRACT
The essential oils from the flower, leaf, stem and root of Nepeta persica Boiss., analyzed by GC and GC/MS, were shown to contain 4abeta,7alpha,7abeta-nepetalactone (58.5%, 62.3%, 66.2% and 27.1%, respectively), and 4aalpha,7alpha,7abeta-nepetalactone (33.0%, 28.3%, 24.9% and 7.6%, respectively). The other main component of the flower and stem oils was alpha-pinene (3.6% and 4.4%) and of the leaf oil beta-ocimene (3.6%). In the root oil, other main constituents were alpha-pinene (40.4%), alpha-amorphene (5.3%), gamma-cadinene (2.9%), and cis-calamenene (2.5%). Nepetalactone was the major component of the flower, leaf and stem oils, which are thus important sources of nepetalactone. Antibacterial activities of the flower, leaf, stem and root oils were evaluated using the micro-dilution broth method. Inhibitory effects on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Enterococcus faecalis were recorded. The flower, leaf, stem, and root oils had difference activities against the test microorganisms. The antibacterial property of the essential oils might be ascribed to their high content of nepetalactone isomers.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Cyclopentanes/isolation & purification , Nepeta/chemistry , Plant Oils/isolation & purification , Pyrones/isolation & purification , Terpenes/isolation & purification , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Cyclopentane Monoterpenes , Cyclopentanes/chemistry , Cyclopentanes/pharmacology , Gas Chromatography-Mass Spectrometry , Microbial Sensitivity Tests , Plant Oils/chemistry , Plant Oils/pharmacology , Pyrones/chemistry , Pyrones/pharmacology , Terpenes/chemistry , Terpenes/pharmacologyABSTRACT
The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-beta-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were alpha-pinene (50.0%), trans-beta-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the micro-dilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.
