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Appl Environ Microbiol ; 69(5): 2484-90, 2003 May.
Article in English | MEDLINE | ID: mdl-12732512

ABSTRACT

Denaturing gradient gel electrophoresis was used as a molecular tool to determine the diversity and to monitor population dynamics of viruses that infect the globally important coccolithophorid Emiliania huxleyi. We exploited variations in the major capsid protein gene from E. huxleyi-specific viruses to monitor their genetic diversity during an E. huxleyi bloom in a mesocosm experiment off western Norway. We reveal that, despite the presence of several virus genotypes at the start of an E. huxleyi bloom, only a few virus genotypes eventually go on to kill the bloom.


Subject(s)
DNA Viruses/isolation & purification , Phytoplankton/virology , Base Sequence , Capsid Proteins/genetics , Conserved Sequence , DNA Viruses/classification , DNA Viruses/genetics , DNA, Viral/genetics , Ecosystem , Genes, Viral , Molecular Sequence Data , Norway , Phylogeny , Seawater/microbiology , Sequence Homology, Nucleic Acid
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