Improved plant heat shock resistance is introduced differently by heat and insect infestation: the role of volatile emission traits.

Heat stress is one of the most important abiotic stresses confronted by plants under global climate change. Plant exposure to abiotic or biotic stress can improve its tolerance to subsequent severe episodes of the same or different stress (stress priming), but so far there is limited comparative information about how pre-exposures to different abiotic and biotic elicitors alter plant resistance to severe heat stress. We exposed the perennial herb Melilotus albus Medik., a species rich in secondary metabolites, to moderate heat stress (35 °C) and greenhouse whitefly (Trialeurodes vaporariorum West.) infestation to comparatively determine whether both pre-treatments could enhance plant tolerance to the subsequent heat shock (45 °C) stress. Plant physiological responses to stress were characterized by photosynthetic traits and volatile organic compound emissions through 72 h recovery. Heat shock treatment reduced net assimilation rate (A) and stomatal conductance in all plants, but heat-primed plants had significantly faster rates of recovery of A than other plants. By the end of the recovery period, A in none of the three heat shock-stressed groups recovered to the control level, but in whitefly-infested plants it reached the pre-heat shock level. In heat-primed plants, the heat shock treatment was associated with a fast rise of monoterpene emissions, and in whitefly-infested plants with benzenoid emissions and an increase in total phenolic content.

Wounding-Induced VOC Emissions in Five Tropical Agricultural Species.

Leaf mechanical wounding triggers a rapid release-within minutes-of a blend of volatile organic compounds. A wounding-induced VOC blend is mainly composed of oxygenated ubiquitous stress volatiles such as methanol and volatile products of lipoxygenase (LOX) pathway (mainly C5 and C6 alcohols and aldehydes and their derivatives), but also includes multiple minor VOCs that collectively act as infochemicals, inducing defences in non-damaged plant leaves and neighbouring plants and attracting herbivore enemies. At present, the interspecific variability of the rate of induction and magnitude of wounding-induced emissions and the extent to which plant structural traits and physiological activity alter these emissions are poorly known. Particularly scarce is information on the induced emissions in tropical agricultural plant species, despite their economic importance and large area of cultivation at regional and global scales. We chose five tropical crops with varying photosynthetic activity and leaf structural characteristics-Abelmoschus esculentus, Amaranthus cruentus, Amaranthus hybridus, Solanum aethiopicum, and Telfairia occidentalis-to characterize the kinetics and magnitude of wounding-induced emissions, hypothesizing that the induced emission response is greater and faster in physiologically more active species with greater photosynthetic activity than in less active species. Rapid highly repeatable leaf wounds (12 mm cuts) were generated by a within-leaf-chamber cutting knife. Wounding-induced VOC emissions were measured continuously with a proton-transfer reaction time-of-flight mass spectrometer and gas-chromatography mass spectrometry was used to separate isomers. Twenty-three ion VOCs and twelve terpenoid molecule structures were identified, whereas ubiquitous stress volatiles methanol (on average 40% of total emissions), hexenal (24%), and acetaldehyde (11%) were the main compounds across the species. Emissions of low-weight oxygenated compounds (LOC, 70% of total) and LOX products (29%) were positively correlated across species, but minor VOC components, monoterpenoids and benzenoids, were negatively correlated with LOC and LOX, indicating a reverse relationship between signal specificity and strength. There was a large interspecific variability in the rate of induction and emission magnitude, but the hypothesis of a stronger emission response in physiologically more active species was only partly supported. In addition, the overall emission levels were somewhat lower with different emission blend compared to the data reported for wild species, as well as different shares for the VOCs in the blend. The study demonstrates that wounding-dependent emissions from tropical agricultural crops can significantly contribute to atmospheric volatiles, and these emissions cannot be predicted based on current evidence of wild plant model systems.