Dynamics of synchronization in the functioning of neurons of the new cortex and hippocampus during learning before and after the administration of nootropic agents and narcotics.

The principal difference in the effect of the preparations investigated resided in the fact that the nootropic agents did not change the effect of painful reinforcement on the synchronicity of neurons of the cerebral cortex of rabbits of the inhibitory type (coincidence of both the presence and absence of impulse activity), and of the activational type (the time of coincidence of only the presence of impulse activity). Both preparations mainly attenuated the initial influences of both the inhibitory and reinforced conditional stimuli on the synchronicity in the activity of the cortical neurons of both the activational and the inhibitory types.

Neurobiology of the integrative activity of the brain. Dynamics of the activational and inhibitory types of synchronization of cortical neurons during the realization of a defensive reflex and of internal inhibition.

An increase in synchronization of the activational type (coincidence of the presence of impulse activity), and a decrease of the inhibitory type (coincidence of both the presence and absence of impulse activity), in the operation of close-lying neurons were observed in the visual and sensorimotor areas of the new cortex and in the hippocampus of wakeful non-immobilized rabbits in response to the combination of flashes of light with electrodermal stimulation (EDS) of the extremity. An increase in the synchronization of the neurons of the inhibitory type took place in the visual cortex in response to flashes against the background of the conditional inhibitor, i.e., continuous light, and changes in synchronization, similar to the effect of pain reinforcement but significantly weaker, appeared in the sensorimotor cortex and in the hippocampus. An increase in synchronicity of the activational type took place primarily in pairs of neurons with increase in the same direction in the frequency of impulse activity in response to a stimulus, and of the inhibitory type, took place with its decrease. In addition, both kinds of changes in synchronization appeared in a significant portion of the pairs of neurons with changes in the frequency of impulse activity of different directions.

[Synchronization dynamics in the neuronal work of the neocortex and hippocampus during learning before and after the administration of nootropics and narcotics]. / Dinamika sinkhroniizatsii v rabote neironov novoi kory i gippokampa pri obuchenii do i posle vvedeniia nootropov i nerkotikov.

The basal difference in action of the studied drugs was that nootropics (phenybut in a dose of 40 mg/kg and pyracetam in a dose of 200-400 mg/kg) did not change the initial action of pain reinforcement on synchronism in responses of the cortical neurones of alert nonimmobilized rabbits by inhibitory type (coincidence of the presence and absence of impulse activity) towards its decrease, while narcotics of various types (ethanol in a dose of 4-6 mg/kg, morphine-like opiate DAGO and opioid peptide DADLE in doses of 250 mkg/kg) eliminated the action of pain reinforcement on synchronism in responses of the cortical neurones both by inhibitory and activation (time of coincidence only of the presence of impulse activity) types. These and other drugs mainly weakened the initial action of both the inhibitory and reinforced light flashes of synchronism in neurones activity both by inhibitory and activation types. There was no constant parallelism between changes of synchronization and the frequency of the cortical impulses.

[The dynamics of the activating and inhibitory types of cortical neuron synchronization during the realization of a defensive reflex and internal inhibition]. / Dinamika aktivatsionnogo i tormoznogo tipov sinkhronizatsii neironov kory pri realizatsii oboronitel'nogo refleksa i vnutrennego tormozheniia.

In the visual and sensorimotor areas of the neocortex and in the hippocampus of alert nonimmobilized rabbits, in response to combinations of light flashes with electrocutaneous limb stimulation an increase was observed of synchronization in the activity of the near-by neurones by activation by inhibitory type (coincidence of the presence and absence of impulse activity). In response to flashes against the light background--conditioned inhibitor--in the visual cortex synchronization of neurones increased by inhibitory type, and in the sensorimotor cortex and hippocampus changes of synchronization appeared, similar to the action of pain reinforcement but considerably weaker. The increase of synchronization by the activation type took place mainly in the neurones pairs with unidirected increase of impulses frequency and by the inhibitory one--with its decrease. Along with this, in a considerable part of neurones pairs both changes of synchronization appeared at the impulses frequency changes of different direction.

The influence of an enkephalin derivative, DAGO, on the behavior and activity of neurons of the neocortex and hippocampus of rabbits during the development of defensive and inhibitory conditioned reflexes.

After the administration of a morphine-like opiate, DAGO (D), in a dose of 250 micrograms/kg, a decrease was observed in the probability of movements of a rabbit in response to light flashes, the signal for a defensive reflex. The level of the background impulse activity of the neurons gradually decreased in the sensorimotor cortex and in the hippocampus, and did not change in the visual cortex. The decrease and the recovery of the responses of the neurons to the reinforcing stimulus (electrodermal stimulation of the limb) proceeded unidirectionally in all of the areas of the cortex studied, while there were substantial differences in the relationship to the cortical area studied and to the biological significance of the stimulus in the dynamics of the responses to the inhibitory and reinforced light flashes. The identification of the features of the systemic organization of the neurons during training with change in the properties of the reinforcement under the influence of the preparation under study is discussed, as well as the similarity of some features in the mechanisms of the development of internal inhibition in the defensive situation and of the properties of positive reinforcement.

[Effect of the enkephalin derivative DAGO on neuronal behavior and activity in the neocortex and hippocampus of rabbits during the acquisition of conditioned defense and inhibitory reflexes]. / Vliianie derivata énkefalina--DAGO na povedenie i aktivnost' neironov novoi kory i gippokampa krolikov pri vyrabotke oboronitel'nykh i tormozhnykh uslovnykh refleksov.

After subcutaneous injection of 25 mkg/kg morphine-like opiate--DAGO a decrease was observed of probabilities of rabbits movements at light flashes--defensive reflex signal. The level of the background neuronal impulse activity became gradually lower in the sensorimotor cortex and the hippocampus and did not change in the visual cortex. Decrease and restoration of responses to the reinforcing stimulus (electrocutaneous limb stimulation) in all studied cortical zones proceeded in one direction while there were significant differences in dynamics of responses to inhibitory and reinforced light flashes depending on the studied cortical zone and biological significance of the stimulus. Appearance is discussed of particular characteristics of neurones systemic organization during learning at change of reinforcement properties under the influence of the studied substance, as well as similarity of some features of mechanisms of internal inhibition elaboration in a defensive situation and of properties of positive reinforcement.

[Effect of piracetam on the neuronal activity of the neocortex and on behavior in rabbits during learning]. / Vliianie piratsetama na aktivnost' neironov novoi kory i povedeniia krolikov pri obuchenii.

Against the background of the action of piracetam--a cyclic derivative of GABA--in a dose of 200-400 mg/kg, no significant changes were observed of probabilities of motor reactions to inhibitory and reinforced light flashes. Piracetam in that dose did not affect inhibitory pauses in responses of neurones in the visual area and corresponding late components of the evoked potential to nonreinforced light flashes, i.e. it did not intensify inhibitory hyperpolarization processes in the cerebral cortex. Piracetam administration improved differentiation of inhibitory and reinforced light flashes judging by bioelectric parameters of the brain activity as a result of intensification of pain reinforcement action on cortical neurones. The carried-out experiments revealed significant differences in neurophysiological mechanisms of action of piracetam and fenibut--GABA linear derivate related to nootropic class.