ABSTRACT
Some effects of glycerol injection on indices of the condition of the thiol-disulfide system as well as carbohydrate and nitrogen metabolism in rats in vivo were studied. A decrease was revealed in levels of non-protein SH-groups in the liver, kidney and heart, as well as of protein SH-groups in the kidney and heart of rats following glycerol injection. That might be connected with SH-group oxidation under the excessive arrival of free haem into tissues under rhabdomyolysis. A decrease in glycogen and increase in tyrosine aminotransferase activity in the liver were observed. Activation of nitrogenous metabolism following glycerol injection is indicated by the increase of aminotransferase activity in organs, and concentration of blood urea. High concentration of creatinine in the rat serum can reflect malfiltration in kidneys.
Subject(s)
Carbohydrate Metabolism , Kidney/metabolism , Liver/metabolism , Muscle, Skeletal/metabolism , Myocardium/metabolism , Nitrogen/metabolism , Rhabdomyolysis/metabolism , Animals , Blood Urea Nitrogen , Creatinine/blood , Glycerol/toxicity , Glycogen/metabolism , Heme/metabolism , Kidney/physiopathology , Liver/physiopathology , Male , Muscle, Skeletal/physiopathology , Oxidation-Reduction , Oxidative Stress , Rats , Rats, Wistar , Rhabdomyolysis/chemically induced , Rhabdomyolysis/physiopathology , Tyrosine Transaminase/metabolismABSTRACT
It was found that the glucose-fatty acids cycle functioned under the oxidative stress, caused by injection of cobalt chloride solution in albino rats. This cycle promoted the adaptation of metabolism and rehabilitated the homeostasis under extreme conditions. Its functioning was regulated by prolonged (during 2-24 hours) rise in activity of amino acids catabolism enzymes (e.g. tyrosine aminotransferase, arginase) and activation of glyconeogenesis after the mobilisation of liver glycogen. This contributed to increase in glucose and free fatty acids contents in blood. The latter is additionally provided by lipid mobilisation under stress. Tyrosine aminotransferase activation occurred both on the transcription level and by enabling of other mechanisms, which probably concerned the stabilisation of this enzyme. Preliminary injection of alpha-tocopherol in vivo significantly decreased the rise in tyrosine aminotransferase and arginase activities and the rate of erythrocyte hemolysis but did not disable them in full. This made evident that in regulation of the glucose-fatty acids cycle not only active metabolites of oxygen but also Co ions were directly enabled.
Subject(s)
Cobalt/toxicity , Fatty Acids/metabolism , Glucose/metabolism , Liver/drug effects , Oxidative Stress/drug effects , Animals , Antioxidants/pharmacology , Arginase/metabolism , Blood Glucose/metabolism , Dactinomycin/pharmacology , Fatty Acids/blood , Liver/enzymology , Liver/metabolism , Male , Rats , Rats, Wistar , Transferases/metabolismABSTRACT
Effect of two plant growth stimulators: bactozol (drug of bacterial origin) and D1 (synthetic analog of phytohormones) on metabolism of pea rhizobia (Rhizobium leguminosarum bv. viciae 2636) and efficiency of their symbiosis with pea plants have been studied. The D1 drug in concentration 0.1% suppressed growth of bacteria. However, bactozol stimulating action on pea rhizobia growth in a pure culture and synthesis of extracellular carbohydrates by them have been established. The trial of three concentrations (0.1%, 0.01%, 0.001%) has shown that bactozol effect has dose-dependent character. The highest effect of stimulation is achieved at concentration 0.1%. Bactozol decreases partially the repressing influence of the mineral nitrogen on growth of rhizobia and their carbohydrate-synthesizing activity under growth of bacteria against a high nitrogen background (20 mM NO3-). Treatment of pea plants by bactozol (0.1%) increases considerably the efficiency of their symbiosis with pea rhizobia, evoking the growth of the overground and root mass of the plants, quantity of nodules and their nitrogenase activity.
