ABSTRACT
Propranolol antihemolytic test parameters calculated with the Hill mathematical model from dose-dependent curves of saturation were studied in 27 patients with myocardial infarction. Erythrocyte beta-adrenoreceptors showed higher sensitivity to propranolol in patients with myocardial infarction within 3 days of the disease. Three weeks after therapy, changes were found in the erythrocyte membranes solely in patients receiving beta-adrenoblockers. The changes are indicative of lower sensitivity of beta-adrenoreceptors to the beta-blocker. Persons with varying responses to propranolol were detected among the patients with myocardial infarction, which may be used to define indications for beta-adrenoblocker therapy.
Subject(s)
Erythrocytes/drug effects , Myocardial Infarction/drug therapy , Propranolol , Receptors, Adrenergic, beta/drug effects , Adrenergic beta-Antagonists/pharmacology , Adrenergic beta-Antagonists/therapeutic use , Adult , Aged , Dose-Response Relationship, Drug , Erythrocyte Membrane/drug effects , Female , Humans , Male , Middle Aged , Myocardial Infarction/bloodABSTRACT
Administration of guanethidine (50 mg/kg) decreased the noradrenaline content in adrenergic neurons which led to an augmentation of the effector organ's adrenoreactivity in rats. The affinity of the erythrocytes' beta-adrenoreceptors to propranolol was enhanced and the parameters of their binding the fluorescent probe were changed. The data obtained suggest the possibility of using the erythrocytes for in vivo estimation of functional state of adrenoreactive system in animals and humans.
Subject(s)
Erythrocyte Membrane/physiology , Receptors, Adrenergic, beta/physiology , Animals , Dose-Response Relationship, Drug , Erythrocyte Membrane/drug effects , Fluorescent Dyes , Guanethidine/pharmacology , Hemolysis/drug effects , Male , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Muscle, Smooth/physiology , Norepinephrine/analysis , Norepinephrine/pharmacology , Propranolol/pharmacology , Rats , Rats, Inbred Strains , Receptors, Adrenergic, beta/drug effectsABSTRACT
The effect of propranolol on hypotonic erythrocyte hemolysis was investigated in normal subjects and coronary patients. In the latter, erythrocytes were shown to have much smaller osmotic stability as compared to the former. Propranolol's in vitro antihemolyzing effect is more pronounced in patients with acute myocardial infarction and angina pectoris as compared to normal subjects. antihemolyzing effect of propranolol is reduced if it is preceded by the administration of beta-adrenoblockers in myocardial infarction. It is suggested that propranolol's antihemolyzing effect be used for the assessment of beta-adrenoreceptor function.
