Developmental temperature alters the thermal sensitivity of courtship activity and signal-preference relationships, but not mating rates.

Mating behaviors are sensitive to novel or stressful thermal conditions, particularly for ectothermic organisms. An organism's sensitivity to temperature, which may manifest in altered mating outcomes, can be shaped in part by temperatures experienced during development. Here, we tested how developmental temperature shapes the expression of adult mating-related behaviors across different ambient conditions, with a focus on courtship behavior, mating rates, and mating signals and preferences. To do so, we reared treehoppers under two temperature regimes and then tested the expression of male and female mating behaviors across a range of ambient temperatures. We found that developmental temperature affects the thermal sensitivity of courtship behavior and mating signals for males. However, developmental temperature did not affect the thermal sensitivity of courtship or mate preferences in females. This sex-specific plasticity did not alter the likelihood of mating across ambient temperatures, but it did disrupt how closely mating signals and preferences matched each other at higher ambient temperatures. As a result, developmental temperature could alter sexual selection through signal-preference de-coupling. We further discuss how adult age may drive sex-specific results, and the potential for mismatches between developmental and mating thermal environments under future climate change predictions.

The potential for the evolution of thermally sensitive courtship behaviours in the treehopper, Enchenopa binotata.

The ability of animals to adapt to warming will depend on the evolutionary potential of thermally sensitive traits. The number of studies measuring the quantitative genetics of a wide variety of thermally sensitive traits has steadily increased; however, no study has yet investigated the quantitative genetics of thermal sensitivity for courtship traits. Since courtship often precedes mating, the ability of these traits to respond to warming may impact reproduction and therefore population persistence. Here, we use classic quantitative genetics breeding design to estimate heritability of various aspects of the thermal sensitivity of courtship behaviours in the treehopper Enchenopa binotata. We generated individual-level thermal courtship activity curves for males and females and measured levels of genetic variation in the thermal sensitivity of courtship activity. We found low heritability with 95% credible intervals that did not approach zero for most traits. Levels of genetic variation were highest in traits describing thermal tolerance. We also found some evidence for genetic correlations between traits within but not across sexes. Together, our results suggest that the range of temperatures over which these treehoppers actively court can evolve, although it remains unclear whether adaptation can happen quickly enough to match the speed of warming.