Effect of heat stress on vital and hematobiochemical parameters of healthy dogs.

Background and Aim: Heat stress is a major challenge for animals, impairing their welfare and performance. This study aimed to determine the effect of heat stress on the vital and hematobiochemical parameters of healthy dogs. Materials and Methods: The experimental subjects comprised 10 dogs, encompassing seven males and three non-pregnant females between 2 and 3 years of age. Ambient temperature (AT) and relative humidity (RH) were recorded 2 hourly during the day and the temperature humidity index was calculated. Vital parameters [i.e., rectal temperature (RT), respiratory rate, and heart rate (HR)] were assessed and blood was collected from each dog daily for hematobiochemical analysis. Results: The RT (38.5±0.2°C) of dogs exposed to high AT and high RH (HA/HR) conditions was significantly (p<0.05) higher than that of dogs exposed to HA and low RH (LR) conditions (37.2±0.11°C). Under HA/HR conditions, packed cell volume, hemoglobin concentrations, and white blood cell counts were significantly lower than those of the same dogs exposed to HA/LR conditions. Conversely, under HA/HR conditions, the lymphocyte, monocyte, eosinophil, alanine aminotransferase, aspartate aminotransferase, and cortisol values were significantly higher (p<0.05) than the values obtained in dogs exposed to HA/LR conditions. Meanwhile, the alkaline phosphatase, urea, and glucose levels were significantly lower (p<0.05) in dogs exposed to HA/HR conditions. Conclusion: The exposure of healthy dogs to HA/HR conditions induced heat stress, which may have an adverse effect on their immune status, thereby affecting their health and welfare.

The Effect of Epicatechin-(4ß-8)-Catechin on Some Biomarkers of Fatigue in Packed Donkeys (Equus Asinus) during the Dry Season in Northern Nigeria.

Overworking and overloading of donkeys may cause fatigue. The experiment aims to determine the effects of epicatechin-(4ß-8)-catechin (EEC) on some fatigue biomarkers in packed donkeys during the dry season. 10 jacks, aged between four and five years were used for the study. Five were given EEC in feed seven days before packing (test), while another five were given feed only (control). Blood samples collected for biochemical analyses were analyzed for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total antioxidant capacity (TAC), creatine kinase (CK) and tumor necrosis factor-alpha (TNF-α). The SOD activity increased significantly (P < 0.05) in the test group during the cold-dry season after packing. The values of TAC during the packing period were significantly (P < 0.05) higher in test group during the hot-dry season. The TNF-α concentration was higher (P > 0.05) in the control group especially one-hour on route. The CK activity was significantly (P < 0.05) higher in the control donkeys during the hot-dry season. In conclusion, packing donkeys during the dry season resulted in fatigue, but EEC served as an anti-fatigue agent.

Ergonomic study of donkeys administered with Pycnogenol® and subjected to packing during the hot-dry season in Northern Nigeria.

The aim of the study was to determine some ergonomic parameters of donkeys administered with Pycnogenol® (PYC) and subjected to packing during the hot-dry season in northern Nigeria. Fifteen donkeys served as experimental subjects. Group 1 (n = 5) donkeys were subjected to trekking only (control), group 2 (n = 5) donkeys were subjected to packing (P-PYC), and group 3 (n = 5) were administered with PYC (10 mg/kg) and subjected to packing (P+PYC). Each group covered a distance of 10 km. Some ergonomic parameters were evaluated. The duration of work in control, P-PYC, and P+PYC donkeys were 170.0 ± 10 min (2.8 h), 126.6 ± 6.7 min (2.1 h), and 112.0 ± 3.9 min (1.9 h), respectively. The energy expenditure obtained for P-PYC group (88.3 ± 9.8 KJ/s) was significantly higher than the values of 28.3 ± 3.7 KJ/s and 73.5 ± 9.9 KJ/s obtained in control and P+PYC groups, respectively. The output was significantly higher in P+PYC group with the value of 5263.2 ± 135 m/h. In conclusion, packing the donkeys during the hot-dry season was stressful; however, PYC may have served as a potent antifatigue agent to reduce the stress.