ABSTRACT
A relacao antigenica de 9 Flavivirus, febre amarela (YF), Wesselsbron (WSL), Uganda S (UGS), Potiskum (POT), West Nile (WN), Banzi (BAN), Zika (ZK), Dengue tipo 1 (DEN-1) e Dengue tipo 2 (DEN-2), foi avaliada por reacao de inibicao da hemaglutinacao cruzada (cross-HI) e reacao de fixacao do complemento cruzada (Cross-CF) entre cada um dos virus e seu fluido ascitico homologo em camundongos. Medias de titulos foram calculadas usando os titulos heterologos e homologos. Reacoes cruzadas CF revelaram maiores variacoes antigenicas entre virus do que reacoes cruzadas HI. Nao houve variacao antigenica significativa entre virus WSL, POT e YF usando cada um dos metodos. Todavia, diferencas definidas da antigenicidade foram observadas entre eles e os virus UGS, BAN e ZK. Nao existiram diferencas significativas entre UGS, BANe ZK ou entre DEN-1 e DEN-2. A relacao sorologica entre Flavivirus e importante para se estabelecer o diagnostico e a epidemiologia destas infeccoes na Africa
Subject(s)
Animals , Mice , Flavivirus/immunology , Flavivirus Infections/immunology , Antigenic Variation/immunology , Flavivirus/isolation & purification , Cross Reactions/immunology , Complement Fixation Tests/methods , Hemagglutination Inhibition Tests/methodsABSTRACT
We describe a case of human T-lymphotropic virus type I associated myelopathy in a 50-year old woman in Nigeria. The patient presented with progressive loss of tone to the two lower limbs and later inability to walk. The HTLV-I antibody presence in the plasma collected from the patient was repeatedly detected by enzyme immunoassays (Abbott HTLV-I EIA and Coulter SELECT-HTLV I/II) and confirmed by Western blot technique. In addition, HTLV-I DNA was amplified from the genomic DNA isolated from the peripheral blood mononuclear cells of the patient by the polymerase chain reaction technique. This finding is significant being the first report of association of HTLV-I with myelopathy in Nigeria.
Subject(s)
DNA Viruses/isolation & purification , Gene Amplification/immunology , HTLV-I Antibodies/blood , Paraparesis, Tropical Spastic/immunology , Blotting, Western , Female , Humans , Middle Aged , Nigeria , Polymerase Chain ReactionABSTRACT
Descrevemos um caso de infecçäo por HTLV-I associado a mielopatia, em mulher de 50 anos, na Nigeria. A paciente apresentou fraqueza progressiva dos membros inferiores e posteriormente incapacidade para andar. A presença de anticorpo HTLV-I no plasma coletado da paciente foi repetidamente detectada pelos ensaios imunoenzimaticos (Abbott HTLV-I EIA e Coulter SELECT-HTLV I/II) e confirmada pela tecnica de Western Blot. Adicionalmente amplificou-se o DNA do HTLV-I a partir do DNA genomico isolado das celulas mononucleares do sangue periferico da paciente através da técnica de PCR. Este achado e significativo sendo o primeiro relato de associaçäo de HTLV-I com mielopatia, na Nigeria
Subject(s)
Humans , Male , Female , Adult , Deltaretrovirus Antibodies/isolation & purification , Leukocytes, Mononuclear/immunology , Paraparesis, Tropical Spastic/diagnosis , Gene Amplification/methods , Blotting, Western , HTLV-I Infections/etiology , Nigeria , Polymerase Chain ReactionABSTRACT
The antigenic relationship of 9 flaviviruses, Yellow fever (YF), Wesselsbron (WSL), Uganda S (UGS), Potiskum (POT), West Nile (WN), Banzi (BAN), Zika (ZK), Dengue type 1 (DEN-1) and Dengue type 2 (DEN-2), was assessed by cross-haemagglutination-inhibition (Cross-HI) and cross-complement fixation (Cross-CF) reactions between each of the viruses and their homologous immune mouse ascitic fluids. Titre ratios were calculated using the heterologous and homologous titres. Cross-CF reactions revealed wider antigenic variations among viruses than Cross-HI reactions. There was no significant antigenic variation between WSL, POT and YF viruses using either of those methods. However, definite differences in antigenicity were observed between them and UGS, BAN and ZK viruses. There were no significant differences between UGS, BAN and ZK or between DEN-1 and DEN-2. The serological relationship among flaviviruses is important in establishing diagnosis and epidemiology of these infections in Africa.