The expression analysis of the estrogen and progesterone receptors in endometrial carcinomas.

The aim of the study was the analysis of expression of estrogen (ER) and progesterone (PR) receptors in endometrial carcinomas. The expression of each of the receptors was examined with reference to such parameters as: patients' age, the relation to menopause, histological type and grading, the depth of myometrial infiltration and the presence of endometrial hyperplasia adjacent to the tumour. There were found very significant correlations between the degree of histological grading and expression of ER and PR and between values of index ER and PR. There was obtained correlation between the expression and endometrial hyperplasia only for PR.

[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer]. / Hydrofobowe addukty DNA w relacji do gestosci receptorów estrogenowych i progesteronowego w gruczolakorakach blony sluzowej macicy u kobiet.

OBJECTIVE: Determination of the relationship between hydrophobic DNA adducts (A) and estrogen receptors (ER) and progesterone (PR) receptor status in uterine cancers. METHODS: Using the P1 enriched version of 32P-postlabeling for hydrophobic DNA adducts detection on polyethyleneimine (PEI) cellulose thin layer chromatograms (TLC) we examined 11 uterine cancer DNAs. The quantification of the adducts was performed by Cerenkov counting of the spots. ER and PR status was recognized histochemically and H-score estimate was performed for each investigated cancer tissue. Patterns of uterine cancer DNA adducts were compared to the maps of adducts recognized in normal human endometrium. RESULTS: In three of the studied uterine cancers there was no positive staining of ER and PR; in one case there was a weak ER staining but PR staining was negative. In ER negative tumors the A level was significantly higher than in ER positive cancers (138.1 +/- 64.1 vs. 49.7 +/- 26.8 adducts per 10(9) nucleotides, respectively, p < 0.05). Highest A levels were found in two ER and PR negative G3 metastatic tumors. Finally, in all investigated cancers there was a strong, inverse correlation between ER content and A level (r = -0.67, p < 0.03). In addition, the correlation between PR level and A was of borderline significance (r = -0.6, p = 0.053). The TLC patterns of adducts in uterine tumors were found to be qualitatively similar, but not quantitatively, to those observed in normal human endometrium DNA. CONCLUSION: The data presented suggest that the hydrophobic DNA adducts could play a role in a sex-steroid hormone independence of human endometrial cancers. The highest accumulation of DNA adducts was recognized in neoplasms displaying the most malignant phenotype.

[Overall genomic DNA methylation in relation to estrogen]. / Metylacja genomowego DNA w relacji do gestosci receptora estrogenowego i progesteronowego w gruczolakorakach blony sluzowej macicy u kobiet.

Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.