ABSTRACT
The article considers results of studying level of transforming growth factor beta 1 (TGF-ß1) in blood plasma of children with terminal stage of hepatic failure before and after transplantation of liver. The relationship of level of cytokine with degree of of liver fibrosis is analyzed. It is demonstrated that level of TGF-ß1 in blood plasma of children with hepatic failure is lower in comparison with healthy children and depends on degree of severity of liver fibrosis. Thus, level of cytokine in blood of patients is higher under fibrosis severity stage II and III, than under fibrosis of severity stage I and IV. After kindred transplantation of hepatic lobe, level of TGF-ß1in blood plasma of patients increases independently of initial degree of severity of fibrosis.
Subject(s)
Electrophoresis/methods , Kidney/metabolism , Proteins/analysis , Proteinuria/urine , Animals , HumansABSTRACT
The authors analyze the pathogenetic significance of hyperhomocysteinemia, antiphospholipid syndrome, the hyperexpression of cell adhesion molecules, inflammation, and oxidative disorders for, as well as the role of viral infections in the development of coronary artery disease of the grafted heart. The paper shows that viral infections in recipients lead to the development of proinflammatory, proatherogenous, and prothrombogenous status, expressing themselves in an increase in the corresponding laboratory markers in recipients' blood plasma, and points out the role of viral infection in the pathogenesis of coronary artery disease of the transplanted heart. Control and treatment of viral infections, as well as pharmacocorrection of proinflammatory, proatherogenous, and prothrombogenous status would made it possible to influence the development of coronary artery disease of the grafted heart.
Subject(s)
Atherosclerosis/complications , Coronary Disease/etiology , Heart Transplantation/pathology , Virus Diseases/complications , Atherosclerosis/pathology , Coronary Disease/pathology , Humans , Postoperative Complications , Risk FactorsSubject(s)
Abortion, Threatened/immunology , Pregnancy Proteins/immunology , Pregnancy/immunology , T-Lymphocytes/immunology , Cells, Cultured/drug effects , Cells, Cultured/immunology , Concanavalin A/pharmacology , Dose-Response Relationship, Immunologic , Female , Humans , Immune Tolerance/drug effects , Immune Tolerance/immunology , Pregnancy Proteins/drug effects , T-Lymphocytes/drug effectsABSTRACT
A comparative analysis of radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques in determining placental protein 14 (PP14) levels in gynecologic patients was performed. Serum PP14 levels were assessed in the patients in the last period of gestation, during menopause and in healthy nonpregnant controls. The possible discrepancy in PP14 levels was also examined in gynecologic patients with benign and malignant tumors. Both RIA and ELISA techniques proved to be sufficiently sensitive to measure PP14 concentration.
Subject(s)
Enzyme-Linked Immunosorbent Assay , Glycoproteins , Pregnancy Proteins/blood , Radioimmunoassay , Female , Fetal Diseases/blood , Glycodelin , Humans , Menopause , Pregnancy , Pregnancy Complications/bloodABSTRACT
Serum levels of the protein factor of fertility (PFF) were measured by immunoenzymatic assay with a sensitivity of 4 ng/ml. The normal concentration of PFF was within 8-20 ng/ml in males and 20-40 ng/ml in females. Elevated levels of PFF--from 50 to 400 ng/ml--were found in patients with ovarian tumors (19%), cancer of the uterine body (25%) and tumors of the uterine cervix (37%).
Subject(s)
Glycoproteins , Neoplasms/blood , Pregnancy Proteins/blood , Animals , Choriocarcinoma/blood , Female , Glycodelin , Humans , Immunoenzyme Techniques , Leiomyoma/blood , Liver Cirrhosis/blood , Liver Neoplasms/blood , Male , Neoplasm Staging , Ovarian Neoplasms/blood , Pregnancy , Psoriasis/blood , Rabbits , Reference Values , Sclerosis/blood , Stomach Neoplasms/blood , Uterine Neoplasms/bloodABSTRACT
An immunoenzyme assay is developed for quantitative estimation of placenta specific alpha 1-microglobulin in biological fluids. The assay is more sensitive as compared with immunodiffusion and radioimmunoassays used previously. Concentration of alpha 1-microglobulin in blood serum of men-donors was found to be 7-30 ng/ml and of women--0.50 ng/ml.
Subject(s)
Alpha-Globulins/analysis , Insulin-Like Growth Factor Binding Proteins , Blood Donors , Female , Humans , Immunoenzyme Techniques , Insulin-Like Growth Factor Binding Protein 1 , Male , Pregnancy Proteins/analysis , Reference ValuesSubject(s)
Alpha-Globulins/analysis , Anovulation/blood , Fertility , Menstrual Cycle , Female , Humans , Infertility, Female/blood , Reference ValuesABSTRACT
A preparation of fertility alpha 2-microglobulin was isolated from an extract of decidual tissue with a purity about 95% and complete maintaining of native properties using chromatography on anion and cation exchange Sephadexes.
Subject(s)
Alpha-Globulins/isolation & purification , Pregnancy Proteins/isolation & purification , Chromatography, Thin Layer , Decidua/analysis , Female , Humans , PregnancyABSTRACT
With monospecific test-system control, preparative fractionation of 9 individual soluble human leucocyte antigens has been carried out using adsorption chromatography with silicagel, kaolin, Al2O3, Ba2SO3 and chromatography with ion-exchange Sephadexes. Optimal conditions for the preparation of purified leucocyte antigens were determined.
Subject(s)
Antigens/isolation & purification , Leukocytes/immunology , Adsorption , Chromatography/methods , Chromatography, Ion Exchange/methods , Humans , Indicators and Reagents , SolubilityABSTRACT
The immunochemical study of the blood sera of children with chronic and relapsing infections has shown an increase in the content of alpha 2-macroglobulin and alpha 1-antitrypsin in the absence of significant changes in the concentration of immunoglobulins and complement components. The immunochemical analysis of the system of soluble leukocytic antigens (SLA) has revealed a decrease in the level of SLA-1 simultaneously with the presence of redundant amounts of SLA-5 and SLA-8.
Subject(s)
Antigens/analysis , Autoantigens/analysis , Blood Proteins/analysis , Furunculosis/immunology , Leukocytes/immunology , Otitis Media/immunology , Respiratory Tract Infections/immunology , Child , Child, Preschool , Chronic Disease , Humans , Immunodiffusion , Recurrence , SolubilityABSTRACT
A new glycoprotein (RAL-6) was identified in human venous blood by immunochemical assay. During studies into the physicochemical properties, it was established that the glycoprotein has a mobility of beta 2-globulins and contains sialic acids. Study of RAL-6 content in tissue extracts from different organs of man and in biological fluids demonstrated the protein to be contained by pus extracts in a large quantity. The concentration of RAL-6 in hemolysates was found to be lowered in some of the diseases including those of the autoimmune nature.
Subject(s)
Beta-Globulins/analysis , Sialic Acids/analysis , Female , Humans , Immunodiffusion , MaleSubject(s)
Alpha-Globulins/physiology , Labor, Obstetric , Adult , Alpha-Globulins/analysis , Decidua/analysis , Female , Humans , Placenta/analysis , PregnancyABSTRACT
Complex differential-diagnostic immunodiffusional test for a number of blood plasma proteins, antigens of liver tissue and alpha-fetoprotein was used to evaluate the liver tissue functional state. The test characterized more distinctly the liver tissue function as compared with estimation of individual proteins and was more informative in diagnosis of liver tissue disorders.
Subject(s)
Antigens/analysis , Blood Proteins/analysis , Liver Diseases/diagnosis , Clinical Laboratory Techniques , Diagnosis, Differential , Humans , Immunodiffusion , Liver Function Tests , alpha-Fetoproteins/analysisABSTRACT
After haemosorption of patients with psoriasis eluates from columns of IGI charcoal were tested for presence of 19 main plasma proteins using monospecific antisera. Distinct selectivity of protein sorption on IGI charcoal was found due to strong adsorption of the proteins responsible for immunity (IgG, IgA, IgM, C3- and C4-components of complement) as well as of fibrinogen.
Subject(s)
Blood Proteins/analysis , Hemoperfusion , Psoriasis/blood , Adsorption , Blood Protein Electrophoresis , Complement C3/analysis , Complement C4/analysis , Fibrinogen/analysis , Humans , Immunodiffusion , Immunoelectrophoresis , Immunoglobulin A/analysis , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Psoriasis/therapyABSTRACT
The diagnostic value of determinations of the levels of chorionic gonadotropin (HCG) and trophoblastic beta 1-glycoprotein (TBG) was studied in 160 cases of trophoblastic disease. Trophoblastic disease was diagnosed in 86% of cases, when HCG level was determined immunologically and in 100 % -- by radioimmunological means and in 86 and 33% when TBG level was established by immunoradioautographical and immunodiffusion methods, respectively. The results of HCG and TBG assays by immunological and immunoradioautographical methods matched in 96% of cases. The said methods are highly specific and may find a wide application in diagnosing trophoblastic disease. The radioimmunological method of HCG assay is the most sensitive. However, in view of possible HCG synthesis in cases of other tumor, its application should be preferred in assessing the efficacy of therapy rather than for primary diagnosis of trophoblastic disease.