ABSTRACT
We studied the role of light during exogenous assimilation of nitrate (as the only source of nitrogen) by the callus cells of soybean (Glycine max). The nitrate absorbed and assimilated by the photosynthetic (mixotrophic) and non-photosynthetic cells (heterotrophic and chlorophyll-containing cells cultivated in the light in the same medium with diuron) was quantified. The assimilated nitrate was quantified at the final stage of the growth cycle as the difference between the amount of nitrogen absorbed from the medium and the amount of endogenous nitrate in the cells. Comparison of the assimilated nitrate quantities per accumulated dry biomass between the photosynthetic and non-photosynthetic cells demonstrated that nearly 30% of nitrate is assimilated with the help of photosynthesis in a mixotrophic culture when nitrate is the only source of nitrogen.
Subject(s)
Glycine max/metabolism , Nitrates/metabolism , Cells, Cultured , Light , Plant Structures/cytology , Plant Structures/metabolism , Glycine max/cytologyABSTRACT
The effect of an aqueous extract from malt sprouts on the growth and development of fodder yeast was investigated during their continuous cultivation. The extract can be used as a nitrogen source during continuous cultivation of fodder yeast. It is supposed that the extract contains compounds capable to inhibit yeast growth. The extract should be added to the nutrient medium in the amount of 4 g/l of dry weight.