ABSTRACT
The immunomodulatory properties of Lactobacillus delbrueckii subsp. bulgaricus IMVB-7281, Lactobacillus casei IMV B-7280, Lactobacillus acidophilus IMV B-7279, Bifidobacterium animales VKL and B. animales VKB strains on the models of experimental staphylococcosis infection in mice were determined. It was found that after the mice, infected with staphylococcus, were treated by some probiotic strains of lacto- and bifidobacteria, a normalization of functional activity of phagoctic cells system and increase of the endogenous interferon production were observed. L. delbrueckii subsp. bulgaricus IMVB-7281, L. casei IMVB-7280, L. acidophilus IMV B-7279, B. animales VKB and B. animales VKL are promising for the development of probiotics, effective against staphylococci and for the immunity correction.
Subject(s)
Bifidobacterium/physiology , Lacticaseibacillus casei/physiology , Lactobacillus acidophilus/physiology , Macrophages, Peritoneal/drug effects , Probiotics/therapeutic use , Staphylococcal Infections/immunology , Administration, Oral , Animals , Antibiosis , Cell Count , Female , Immunomodulation/drug effects , Injections, Intraperitoneal , Interferons/biosynthesis , Interferons/immunology , Killer Cells, Natural/drug effects , Killer Cells, Natural/immunology , Macrophages, Peritoneal/immunology , Mice , Mice, Inbred BALB C , Peritoneum/drug effects , Peritoneum/immunology , Peritoneum/microbiology , Probiotics/administration & dosage , Spleen/drug effects , Spleen/immunology , Spleen/microbiology , Staphylococcal Infections/microbiology , Staphylococcal Infections/therapy , Staphylococcus/drug effectsABSTRACT
Spring rapeseed transgenic lines expressing human interferon alpha 2b were created by Agrobacterium-mediated transformation of aseptic plant leaf explants. The maximum antiviral activity of the leaf extracts reached 4500 IU/g fresh weight. It was determined that the antioxidant activity and the activity of an enzyme of plant antioxidant system--superoxide dismutase (SOD)--in the leaf tissues of transgenic plants increased compared to controls. There were no correlations between the interferon and antioxidant activities, as well as between SOD and interferon activities. Using the obtained transgenic rapeseed plants with high interferon and antioxidant activities as a feed additive for animals might have preventive effect on their body, increasing resistance to infections of various origins.
