ABSTRACT
SUMMARY: Refixation of the damaged acetabular labrum is a method of surgical treatment of the hip joint that can promote the repair of joint function after injury and prevent premature osteoarthritis. We sought to determine the condition of the hip joint in rabbits 4 months after excision of the acetabular labrum and the condition of the joint after labral refixation. The articular cartilage of the femoral head and acetabulum was examined by histological methods, multipoint measurement of cartilage thickness, and the ratio between cartilage matrix and chondrocytes lacunae, and the condition of cartilage according to the OARSI grading scale was carried out. On this model, a correlation analysis was performed between the results of the OARSI grading scale and the data of linear morphometry. All these parameters made it possible to better assess changes in articular cartilage. The ratio between matrix and chondrocyte lacunae turned out to be a method that allows establishing early cartilage damage when erosion, fibrosis or deformation did not occur. We found significant differences between the condition of the cartilage after exicion of acetabular labrum and after labral refixation, which give hope to confirm that this surgical technique can delay or prevent progressive changes in the cartilage of the damaged hip joint.
La refijación del labrum acetabular dañado es un método de tratamiento quirúrgico de la articulación coxal, que puede promover la reparación de la función articular después de una lesión y prevenir la osteoartritis prematura. Intentamos determinar el estado de la articulación coxal en conejos de 4 meses después de la escisión del labrum acetabular y observar el estado de la articulación después de la refijación del labrum. El cartílago articular de la cabeza femoral y el acetábulo se examinó por métodos histológicos, se midió a través de multipunto el grosor del cartílago y se realizó la relación entre la matriz del cartílago y las lagunas de condrocitos, y se llevó a cabo la condición del cartílago según la escala de clasificación OARSI. Sobre este modelo se realizó un análisis de correlación entre los resultados de la escala de calificación OARSI y los datos de la morfometría lineal. Todos estos parámetros permitieron evaluar mejor los cambios en el cartílago articular. La relación entre la matriz y las lagunas de condrocitos resultó ser un método que permite establecer temprano el daño del cartílago cuando no se presentó erosión, fibrosis o deformación. Encontramos diferencias significativas entre la condición del cartílago después de la extirpación del labrum acetabular y después de la refijación del labrum, lo que da la esperanza de confirmar que esta técnica quirúrgica puede retrasar o prevenir cambios progresivos en el cartílago de la articulación coxal dañada.
Subject(s)
Animals , Rabbits , Cartilage, Articular , Femur Head , Hip Joint , Acetabulum/surgeryABSTRACT
The article presents the results of an experimental study on the knee joint structural changes and morphometric evaluation of articular cartilage with the injections of platelet rich plasma (PRP) and bone marrow aspirate concentrate (BMAC). Cartilage defect, anterior cruciate ligament intersection and medial meniscus resection were performed for the induction of osteoarthritis in rabbits. After 28 days, 0.9% NaCl, PRP or BMAC was administered intra-articularly; 0.9% NaCl or PRP was injected intra-articularly within 14 days. After 2 months, histological and morphometric examination of the epiphyseal surface of the femur and tibia was performed. Animal models showed identical diameter of epiphyseal cartilage defect, chondrocyte reduction (by 55.1%), reduction of perifocal articular surface thickness (by 53.2%) and relative bone density of epiphysis (by 44.3%). Modeling of the joint defect was not limited to the area of damage and caused degenerative changes of the articular cartilage of the tibia. Statistical analysis showed that the introduction of PRP had no preventive effect on the degenerative changes in the articular surface. In the BMAC group, the thickness of the hyaline cartilage of the tibia was found to be 23.1% (P<0.05) greater compared with the 0.9% NaCl group and the double PRP group. However, the relative density of subchondral bone tissue in the experimental groups with osteoarthritis had no differences.
