Copper multifaceted interferences during swine wastewater treatment in high-rate algal ponds: alterations on nutrient removal, biomass composition and resource recovery.

Microalgae cultivation in swine wastewater (SW) allows the removal of nutrients and biomass production. However, SW is known for its Cu contamination, and its effects on algae cultivation systems such as high-rate algal ponds (HRAPs) are poorly understood. This gap in the literature limits the proposition of adequate concentrations of Cu to optimise SW treatment and resource recovery in HRAPs. For this assessment, 12 HRAPs installed outdoors were operated with 800 L of SW with different Cu concentrations (0.1-4.0 mg/L). Cu's interferences on the growth and composition of biomass and nutrient removal from SW were investigated through mass balance and experimental modelling. The results showed that the concentration of 1.0 mg Cu/L stimulated microalgae growth, and above 3.0 mg Cu/L caused inhibition accompanied by an accumulation of H2O2. Furthermore, Cu affected the contents of lipids and carotenoids observed in the biomass; the highest concentration was observed in the control (16%) and 0.5 mg Cu/L (1.6 mg/g), respectively. An innovative result was verified for nutrient removal, in which increased Cu concentration reduced the N-NH4+ removal rate. In contrast, the soluble P removal rate was enhanced by 2.0 mg Cu/L. Removal of soluble Cu in treated SW reached 91%. However, the action of microalgae in this process was not associated with assimilation but with a pH increase resulting from photosynthesis. A preliminary evaluation of economic viability showed that the commercialisation of biomass considering the concentration of carotenoids obtained in HRAPs with 0.5 mg Cu/L could be economically attractive. In conclusion, Cu affected the different parameters evaluated in this study in a complex way. This can help managers consort nutrient removal, biomass production, and resource recovery, providing information for possible industrial exploitation of the generated bioproducts.

Swine wastewater treatment in high rate algal ponds: Effects of Cu and Zn on nutrient removal, productivity and biomass composition.

This study aimed to evaluate the simultaneous interferences of Cu and Zn found in swine wastewater (SW) in the development of microalgae considering real conditions of cultivation in high rate algal ponds (HRAPs). Ten HRAPs on a pilot scale were fed with SW with different mixtures of Cu (0.5-3.0 mg/L) and Zn (5.0-25.0 mg/L). The interferences of these metals in removing nutrients (N-NH4+ and soluble phosphorus (Ps)) from the SW were determined. In addition, this study evaluated the effects on biomass growth and biochemical composition. Chlorella sp. was dominant in all HRAPs and the condition that potentiated its growth occurred in medium containing 1.8 mg Cu/L + 15.0 mg Zn/L, while higher concentrations conferred inhibition. Only Cu compromised the removal rates of N-NH4+ while the effects of Zn were not significant. Contrary, Zn interfered with Ps removal rates, but the impact of Cu was not significant. The greatest Cu applications increased the protein levels by biomass (50.5-55.2 %). Carbohydrate accumulation was favored by conditions that inhibited the development of microalgae due to either limitation or excess of metals. Copper and Zn compromised the levels of lipids, and the control treatment had the highest content (24.5 %). The presence of Cu and Zn changed the dynamics of HRAPs regarding nutrient removal, productivity, and biochemical composition of the biomass.

Valorization of swine wastewater in a circular economy approach: Effects of hydraulic retention time on microalgae cultivation.

To optimize the swine wastewater (SWW) treatment, this study investigated different hydraulic retention times (HRTs) for microalgae cultivation. For this purpose, five pilot-scale reactors operated in semi-continuous flow, with HRTs equal to 9, 12, 15, 18, 21 days were evaluated in terms of SWW polishing and biomass production. The effluent treatment was discussed accompanied by principal component analysis, which allowed identification of causes of variance in the data set, ideal for studies with real effluent and influenced by environmental conditions. All reactors show satisfactory removals of N-NH4+ (91.6-95.3%), COD (15.8-39.9%), DO increment (in average 7.5 mg O2/L) and, only the longest HRT (21 days) was able to remove Ps (21%). The results obtained indicated that a consortium of microalgae and bacteria was developed for all the tested HRTs. On the other hand, HRT = 12 days provided a healthier culture of photosynthesizing organisms (chl-a/VSS = 3.04%). Carbohydrates (20.8-31.3%) and proteins (2.7-16.2%) were the compounds of commercial interest in the highest proportion in the biomass of all reactors, with contents comparable to that of terrestrial crops. Thus, it was suggested a valorization route of these compounds of high added value to return to pig farming, where the nutrients were intended to supplement the swine feed and clarified water for cleaning the pig stalls. Thus, in the circular economy context, this research contributes to water footprint reduction and the sustainability of the pig farming production chain. The economic and environmental analysis of the route is suggested to enable its implementation on a large scale, as well as further technical feasibility research (reactor types, exposure to external environment, evaluation of pathogen removal and animal feed supplementation from SWW microalgae biomass).

Innovative microalgae biomass harvesting methods: Technical feasibility and life cycle analysis.

In order to ease one of the main challenges of biomass production in wastewater, the harvest stage, this study proposes as main innovations: the comparison of technical and environmental performance of different methods of harvesting biomass which have not been addressed in the literature and the projection of an optimal environmental scenario for biomass harvesting. For this, three harvesting methods were evaluated and compared, namely the gravitational sedimentation (GS) via settling tank, coagulation with tannin followed by gravitational sedimentation (TC/GS), and a biofilm reactor operated in parallel with a settling tank (BR/GS). TC/GS required less time to concentrate the biomass (121.13 g/day); however, the biomass had a higher moisture content (99.02%), which may compromise its direct application for production of most bioproducts and bioenergy, only a dewatering step is recommended. The harvesting methods interfered in biomass characterisation, mainly in carbohydrate content, which was higher in biomass concentrated over time (28-37%) than biomass concentrated in a single day by coagulation (13.8%). The results of the life cycle assessment revealed that in scenarios which included TC/GS methods and the BR/GS presented less environmental impact in relation to only GS. Additionally, the combination of these two methods comprises the best scenario and promises to optimise the harvest of biomass grown in wastewater.

Determination of the spatial susceptibility to Yellow Fever using a multicriteria analysis.

BACKGROUND: The outbreak of sylvatic Yellow Fever (SYF) in humans during 2016-2017 in Brazil is one of the greatest in the history of the disease. The occurrence of the disease in areas with low vaccination coverage favoured the dissemination of the disease; therefore, it is necessary to identify the areas vulnerability to the YF virus (YFV) to assist in the adoption of preventive measures. OBJECTIVE: To correlate the physical-environmental elements associated with the occurrence of SYF in humans via a multicriteria analysis. METHODS: For the multicriteria analysis, preponderant elements related to SYF occurrences, including soil usage and coverage, temperature, precipitation, altitude, mosquito transmitters, and non-human primate occurrence areas, were considered. The results were validated by assessing the correlation between the incidence of SYF and the vulnerable areas identified in the multicriteria analysis. RESULTS: Two regions with different vulnerability to the occurrence of the disease were identified in the multicriteria analysis, with emphasis on the southern areas of the state of São Paulo northeast areas of Minas Gerais, and the entire states of Rio de Janeiro and Espírito Santo. The map of SYF vulnerability obtained in the multicriteria analysis coincides with the areas in which cases of the disease have been recorded. The regions that presented the greatest suitability were in fact the municipalities with the highest incidence. MAIN CONCLUSIONS: The multicriteria analysis revealed that the elements that were used are suited for and consistent in the prediction of the areas that are vulnerable to SYF. The results obtained indicate the proximity of the areas that are most vulnerable to the disease to densely populated areas where an Aedes aegypti infestation was observed, which confers a high risk of re-urbanisation of YF.

Determination of the spatial susceptibility to Yellow Fever using a multicriteria analysis

BACKGROUND The outbreak of sylvatic Yellow Fever (SYF) in humans during 2016-2017 in Brazil is one of the greatest in the history of the disease. The occurrence of the disease in areas with low vaccination coverage favoured the dissemination of the disease; therefore, it is necessary to identify the areas vulnerability to the YF virus (YFV) to assist in the adoption of preventive measures. OBJECTIVE To correlate the physical-environmental elements associated with the occurrence of SYF in humans via a multicriteria analysis. METHODS For the multicriteria analysis, preponderant elements related to SYF occurrences, including soil usage and coverage, temperature, precipitation, altitude, mosquito transmitters, and non-human primate occurrence areas, were considered. The results were validated by assessing the correlation between the incidence of SYF and the vulnerable areas identified in the multicriteria analysis. RESULTS Two regions with different vulnerability to the occurrence of the disease were identified in the multicriteria analysis, with emphasis on the southern areas of the state of São Paulo northeast areas of Minas Gerais, and the entire states of Rio de Janeiro and Espírito Santo. The map of SYF vulnerability obtained in the multicriteria analysis coincides with the areas in which cases of the disease have been recorded. The regions that presented the greatest suitability were in fact the municipalities with the highest incidence. MAIN CONCLUSIONS The multicriteria analysis revealed that the elements that were used are suited for and consistent in the prediction of the areas that are vulnerable to SYF. The results obtained indicate the proximity of the areas that are most vulnerable to the disease to densely populated areas where an Aedes aegypti infestation was observed, which confers a high risk of re-urbanisation of YF.