Perfil dos hormônios tireoidianos de mulheres acima de 50 anos atendidas em um laboratório de referência de Serrinha-BA / Thyroid hormone profile of women over 50 years old at a reference laboratory on Serrinha-BA

Objetivo: Caracterizar o perfil dos hormônios tireoidianos de mulheres com idade maior ou igual a 50 anos atendidas no Laboratório Municipal de Referência Regional de Serrinha (LMRRS) no ano de 2017. Métodos: Os resultados de T3, T4 livre e TSH foram coletados retrospectivamente através do sistema informatizado utilizado pelo laboratório para construção de uma base de dados, e analisados de acordo com valores de referência recomendados pela Sociedade Brasileira de Endocrinologia. Resultados: Foi encontrada uma prevalência moderada de disfunções tireoidianas, sendo 58,62% de casos de hipotireoidismo e 41,38% de hipertireoidismo. Houve também aumento dos níveis de TSH e T4 livre entre os 50 e 70 anos, sem alterações nos níveis de T3 total. Conclusão: Os resultados assemelham-se a estudos anteriores, envolvendo outras populações, com predomínio do hipotireoidismo subclínico entre mulheres na mesma faixa etária. No entanto, a tendência crescente dos níveis de T4 livre não reflete os achados prévios que associam o avanço da idade e redução da produção de estrogênios com a diminuição da função tireoidiana.

Objective: To characterize the thyroid hormone profile of women aged 50 years and older attended at the Laboratório Municipal de Referência Regional de Serrinha (LMRRS) in the year 2017. Methods: The results of T3, free T4 and TSH were retrospectively collected from the computerized system of laboratory for building a database, and analyzed according to the reference values recommended by the Brazilian Society of Endocrinology. Results: A moderate prevalence of thyroid dysfunction was found, with 58.62% of cases of hypothyroidism and 41.38% of hyperthyroidism. There was also an increase of TSH and free T4 levels between 50 and 70 years with no changes in total T3 levels. Conclusion: The results are similar to previous studies involving other populations, with a prevalence of subclinical hypothyroidism in women in the same age group. However, the increasing trend of free T4 levels does not reflect the previous findings that associate the advancement of age and reduction of estrogen production with the decrease of thyroid function.

Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides.

CONTEXT: Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness. OBJECTIVE: This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties. MATERIALS AND METHODS: Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and ß-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle. RESULTS AND DISCUSSION: Essential oils from leaves of L. thymoides had the sesquiterpene ß-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC = 0.004 mg/mL and MMC = 0.26-10.19 mg/mL) and Micrococcus luteus (MIC = 0.03 mg/mL and MMC = 8.43 mg/mL), relax isolated rat aorta (EC50 = 305-544 µg/mL, with endothelium; and EC50 = 150-283 µg/mL, without endothelium), and uterus (EC50 = 74-257 µg/mL), and minor potency, isolated guinea-pig trachea. CONCLUSIONS: Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities.

Pharmacological Basis for Traditional Use of the Lippia thymoides.

The aim of this study was to evaluate crude extracts and fractions from leaves and stems of Lippia thymoides and to validate their use in folk medicine. In vitro antioxidant and antimicrobial activities and in vivo wound healing in rats, baker yeast-induced fever in young rats, and acute oral toxicity in mice assays were realized. The crude extracts and their dichloromethane and ethyl acetate fractions had potent radical-scavenging activity against the DPPH but were not effective in the ß-carotene bleaching method. The dichloromethane fraction from the leaves extract showed the broadest spectrum of activity against S. aureus, B. cereus, and C. parapsilosis. The animals treated with crude extracts showed no difference in wound healing when compared with the negative control group. The crude extract from leaves (1200 mg/kg) has equal efficacy in reducing temperature in rats with hyperpyrexia compared to dipyrone (240 mg/kg) and is better than paracetamol (150 mg/kg). In acute toxicity test, crude extract of leaves from Lippia thymoides exhibited no mortality and behavioral changes and no adverse effects in male and female mice. This work validates the popular use of Lippia thymoides for treating the wound and fever, providing a source for biologically active substances.