ABSTRACT
Increasing evidence suggests that flavones can modulate memory and anxiety-like behaviour. However, these therapeutic effects are inconsistent and induce of adverse effects, which have been associated with interactions at the Benzodiazepine (BZ)-binding site. To improve our understanding of flavone effects on memory and anxiety, we employed a plus-maze discriminative avoidance task. Furthermore, we evaluated the potential of the compounds in modulating GABAA receptors via BZ-binding site using molecular modelling studies. Adult male Wistar rats were treated 30â¯min before training session with Vicenin-2 (0.1 and 0.25â¯mg/kg), Vitexin (0.1 and 0.25â¯mg/kg), Isovitexin (0.1 and 0.25â¯mg/kg) and 0.1â¯mg/kg 6-C-glycoside-Diosmetin, vehicle and a GABAA receptor agonist. The analysis of the time spent in the non-aversive vs aversive enclosed arms during the test session and percentage of time in the open arms within the training session revealed that treatment with Isovitexin and 6-C-glycoside-Diosmetin had memory-enhancing and anxiolytic-like effects (Pâ¯<â¯0.001). In contrast, treatment with a higher dose of Diazepam impaired short-and long-term memory when it alleviated anxiety level. Docking studies revealed that flavones docked in a very similar way to that observed to the Diazepam, except by a lack of interaction in residue α1His101 in the BZ-binding site on GABAA receptors, which may be related to memory-enhancing effect. The occurrence of the α1His101 interaction could justify the memory-impairing observed following Diazepam treatment. These findings provide the first evidence that Isovitexin and 6-C-glycoside-Diosmetin could exert their memory-enhancing and anxiolytic-like effects via GABAA receptor modulation, which likely occurs via their benzodiazepine-binding site.
Subject(s)
Anti-Anxiety Agents/pharmacology , Apigenin/pharmacology , Behavior, Animal/drug effects , Brain/drug effects , Cognition/drug effects , Diazepam/pharmacology , Flavonoids/pharmacology , Nootropic Agents/pharmacology , Receptors, GABA-A/drug effects , Animals , Anti-Anxiety Agents/chemistry , Anti-Anxiety Agents/metabolism , Apigenin/chemistry , Apigenin/metabolism , Avoidance Learning/drug effects , Binding Sites , Brain/metabolism , Diazepam/chemistry , Diazepam/metabolism , Discrimination Learning/drug effects , Dose-Response Relationship, Drug , Flavonoids/chemistry , Flavonoids/metabolism , Locomotion , Male , Maze Learning/drug effects , Memory, Long-Term/drug effects , Memory, Short-Term/drug effects , Molecular Docking Simulation , Nootropic Agents/chemistry , Nootropic Agents/metabolism , Protein Binding , Protein Conformation , Rats, Wistar , Receptors, GABA-A/chemistry , Receptors, GABA-A/metabolism , Time FactorsABSTRACT
Abstract Objective To evaluate the food frequency and nutritional status among students according to participation in the Bolsa Família program funded by the government. Methods Cross-sectional study carried out with students from the fourth grade of elementary school in the municipal capital of the southeastern region of Brazil. Food consumption and anthropometry were investigated by a questionnaire administered in school, while participation in the Bolsa Família program and other socio-economic information was obtained through a protocol applied to mothers/guardians. Statistical analysis included the Mann–Whitney test, the chi-squared test, and Poisson regression with robust variance, and the 5% significance level was adopted. Results There were 319 children evaluated; 56.4% were male, with a median of 9.4 (8.6–11.9) years, and 37.0% were beneficiaries of Bolsa Família program. Between the two groups, there was high prevalence of regular soda consumption (34.3%), artificial juice (49.5%), and sweets (40.3%), while only 54.3% and 51.7% consumed fruits and vegetables regularly, respectively. Among participants of Bolsa Família program, a prevalence 1.24 times higher in the regular consumption of soft drinks (95% CI: 1.10–1.39) was identified compared to non-beneficiaries. The prevalence of overweight was higher in the sample (32.9%), with no difference according to participation in the program. Conclusion The study found increased consumption of soft drinks among BFP participants. The high rate of overweight and poor eating habits denote the need to develop actions to promote healthy eating, especially for the beneficiaries of the Bolsa Família program, to promote improvements in nutritional status and prevent chronic diseases throughout life.
Resumo Objetivo Avaliar a frequência alimentar e estado nutricional entre escolares segundo a participação no programa governamental Bolsa Família (PBF). Metodologia Estudo de delineamento transversal realizado com alunos do 4° ano do ensino fundamental da rede municipal de uma capital da região sudeste do Brasil. O consumo alimentar e antropometria foram investigados mediante formulário aplicado com os escolares, enquanto a participação no PBF e outras informações socioeconômicas foram obtidas por meio de protocolo aplicado às mães/responsáveis. A análise estatística contemplou os testes Mann–Whitney, Qui-quadrado e Regressão de Poisson com variância robusta, sendo adotado o valor de significância de 5%. Resultados Foram avaliadas 319 crianças, 56,4% do sexo masculino, com mediana de 9,4 (8,6–11,9) anos, sendo 37,0% beneficiários do PBF. Entre os dois grupos avaliados (participantes e não-participantes do PBF), observou-se elevada prevalência de consumo regular de refrigerante (34,3%), suco artificial (49,5%), e guloseimas (40,3%), enquanto apenas 54,3% e 51,7% consumiram frutas e hortaliças regularmente, respectivamente. Entre os participantes do PBF, identificou-se prevalência de 1,24 vezes maior no consumo regular de refrigerantes (IC 95%: 1,10–1,39), comparado aos não beneficiários. A prevalência de excesso de peso foi elevada na amostra (32,9%), sem diferença segundo a participação no programa. Conclusão O estudo revelou maior consumo de refrigerantes entre participantes do PBF. A alta taxa de excesso de peso e hábitos alimentares inadequados denota a necessidade de desenvolver ações de promoção da alimentação saudável, em especial com os beneficiários do PBF, a fim de promover melhorias nas condições nutricionais e prevenir doenças crônicas ao longo da vida.
