ABSTRACT
Introduction: The COVID-19 outbreak exposes healthcare workers to an increased risk of distress and psychiatric symptoms. Objectives: To evaluate psychological suffering and mental disorders among healthcare workers at a tertiary hospital, a referral center for COVID-19 treatment. Methods: An observational, cross-sectional, quantitative study with descriptive methodology. Fifty-eight healthcare workers who attended consultations at the hospital's Mental Health Outpatient Clinic were included. The study was carried out after approval by the research ethics committee at the Faculdade de Medicina de São José do Rio Preto (32665020.3.0000.5415). Results: 81% were women, mean age was 38.98±10.6 years, 20 (34.5%) were administrative staff, 24 (41.4%) were attending a first consultation, and 28 had had previous psychiatric attention at other services. Sixteen (28%) reported new symptoms during the pandemic, with anxious (10), irritable (3), and depressive (2) symptoms being the most frequent. Anxiety (26) and depressive disorders (19) were the most prevalent. As for exposure to news, the most common feelings were fear (19) and anguish or concern (9). The most common feelings associated with the pandemic were fear and recurrent thoughts of social and economic impact (27). The main reflections were about the meaning of life (17), human vulnerability (11), and the importance of the family (7). Regarding prospects for the future, 70.7% (41) reported hope for improvement. Conclusions: Initial data suggest a high prevalence of anxiety and depressive symptoms, as well as sleep disturbances, regardless of work team. Fear of death and uncertainty about the future are also prevalent. These data reinforce the importance of developing strategies to reduce the risks to this population's mental health.
ABSTRACT
OBJETIVO: Avaliar o efeito da exposição ao estresse pós-privação de sono em características celulares e nucleares do fígado de ratos. MÉTODOS: 16 ratos (Wistar) machos adultos (200-260 g) foram mantidos em ciclo de luz controlado recebendo dieta com quantidades usuais de sal e livre acesso à água e alimento. Os animais foram divididos em dois grupos de oito animais (grupo experimental e grupo controle). Os animais permaneceram na mesma gaiola (dois de cada vez) durante sete dias e, após esse período, foram pesados e separados. O animal do grupo controle continuou na mesma gaiola e o animal do grupo experimental foi colocado em aparato de privação de sono. Após as 96 horas, os dois ratos foram pesados,anestesiados, sacrificados com dose excessiva de anestésico e os fígados foram retirados. RESULTADOS: Não há diferença significante entre o grupo experimental e grupo controle em relação ao peso, embora haja diminuição de peso no grupo experimental. Nas análises cariométricas, houve diferença significante em relação ao diâmetro menor (p=0,03) e volume (p=0,04) do núcleo dos hepatócitos do grupo experimental. Nas análises esteriológicas, houve diferença significante do grupo experimental no núcleo (maior, p=0,036), do citoplasma (menor p=0,009) e outras estruturas (maior p=0,008). CONCLUSÃO: O estresse parece contribuir para alteração na estrutura celular hepática.
OBJECTIVE: To evaluate the effect of stress induced by sleep deprivation on nuclear and cellular features of rat liver. METHODS: 16 adult male Wistar rats (200-260 g) in controlled light cycle received diet with customary salt quantity and free access to water and food. The animals were divided into two groups with 8 animals each (experimental group and control group). Animals stayed in the same box (two at a time) during seven days andafter that period they were weighted e separated. The control group animal continued in the same box and the experimental group animal were transferred to a sleep deprivation apparatus. After the 96 hours the animals were sacrificed by an excessive anesthetic dose; animals were weighted and their liver extracted. RESULTS: There was no significant difference between experimental and control group regarding weight, although therewas a decrease of weight on the experiment group. Karyotipical analysis showed significant smaller diameter (p=0.03) and volume (p=0.044) of hepatocyte nuclei in experimental group. Stereological analyses showed significant differences in experimental group for nucleus (larger, p=0.036), cytoplasm (smaller, p=0.009) and other structures (larger, p=0.008). CONCLUSION: Stress seems to contribute to alteration of hepatic cell structure.
