Reliability analysis of stabilisation pond systems.

This article presents a reliability analysis of 116 full-scale pond systems in Brazil, comprising 73 primary facultative ponds and 43 anaerobic -facultative pond systems. A methodology developed by Niku et al. (1979) is used for the determination of the coefficients of reliability, in terms of the compliance of effluent BOD, COD, TSS and FC to discharge standards or effluent quality targets. The design concentrations necessary to meet the prevailing discharge standards and the expected compliance percentages have been calculated from the coefficients of reliability obtained. The results showed that few units, under the observed operating conditions, would be able to present reliable performances in terms of compliance with the analyzed standards. For the four constituents (BOD, COD, TSS and FC) and both systems (facultative ponds and anaerobic-facultative systems), the variability of the effluent quality was very large, leading to a high variability of the coefficient of variation (CV) and the coefficient of reliability (COR). The effluent quality from the facultative ponds showed a larger distance to both the desired values and the discharge standard values, compared with the anaerobic-facultative systems.

Experimental murine mycobacteriosis: evaluation of the functional activity of alveolar macrophages in thalidomide- treated mice.

Thalidomide is a selective inhibitor of tumor necrosis factor-alpha (TNF-alpha), a cytokine involved in mycobacterial death mechanisms. We investigated the role of this drug in the functional activity of alveolar macrophages in the presence of infection induced by intranasal inoculation of Mycobacterium avium in thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 10(6) M. avium by the respiratory route. Thirty animals received daily thalidomide (30 mg/kg mouse) and 30 received water by gavage up to sacrifice. Ten non-inoculated mice were used as a control group. Lots of animals from each group were evaluated until 6 weeks after inoculation. Infection resulted in an increased total number of inflammatory cells as well as increased activity of pulmonary macrophages. Histologically, intranasal inoculation of bacilli resulted in small mononuclear infiltrates located at the periphery of the organ. Culture of lung fragments revealed the presence of bacilli only at the beginning and at the end of the experimental period. Thalidomide administration did not affect the microbiological or histological features of the infection. Thalidomide-treated and untreated animals showed the same amount of M. avium colonies 3 weeks after infection. Although it did not affect bacillary clearance, thalidomide administration resulted in a decreased percent of spread cells and release of hydrogen peroxide, suggesting that factors other than TNF-alpha play a role in the killing of mycobacteria by alveolar macrophages. Thalidomide administration also reduced the number of spread cells among resident macrophages, suggesting a direct effect of the drug on this phenomenon.

Experimental murine mycobacteriosis: evaluation of the functional activity of alveolar macrophages in thalidomide- treated mice

Thalidomide is a selective inhibitor of tumor necrosis factor-alpha (TNF-alpha), a cytokine involved in mycobacterial death mechanisms. We investigated the role of this drug in the functional activity of alveolar macrophages in the presence of infection induced by intranasal inoculation of Mycobacterium avium in thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 10(6) M. avium by the respiratory route. Thirty animals received daily thalidomide (30 mg/kg mouse) and 30 received water by gavage up to sacrifice. Ten non-inoculated mice were used as a control group. Lots of animals from each group were evaluated until 6 weeks after inoculation. Infection resulted in an increased total number of inflammatory cells as well as increased activity of pulmonary macrophages. Histologically, intranasal inoculation of bacilli resulted in small mononuclear infiltrates located at the periphery of the organ. Culture of lung fragments revealed the presence of bacilli only at the beginning and at the end of the experimental period. Thalidomide administration did not affect the microbiological or histological features of the infection. Thalidomide-treated and untreated animals showed the same amount of M. avium colonies 3 weeks after infection. Although it did not affect bacillary clearance, thalidomide administration resulted in a decreased percent of spread cells and release of hydrogen peroxide, suggesting that factors other than TNF-alpha play a role in the killing of mycobacteria by alveolar macrophages. Thalidomide administration also reduced the number of spread cells among resident macrophages, suggesting a direct effect of the drug on this phenomenon.

Experimental murine mycobacteriosis: evaluation of the functional activity of alveolar macrophages in thalidomide- treated mice

Thalidomide is a selective inhibitor of tumor necrosis factor-a (TNF-a), a cytokine involved in mycobacterial death mechanisms. We investigated the role of this drug in the functional activity of alveolar macrophages in the presence of infection induced by intranasal inoculation of Mycobacterium avium in thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 106 M. avium by the respiratory route. Thirty animals received daily thalidomide (30 mg/kg mouse) and 30 received water by gavage up to sacrifice. Ten non-inoculated mice were used as a control group. Lots of animals from each group were evaluated until 6 weeks after inoculation. Infection resulted in an increased total number of inflammatory cells as well as increased activity of pulmonary macrophages. Histologically, intranasal inoculation of bacilli resulted in small mononuclear infiltrates located at the periphery of the organ. Culture of lung fragments revealed the presence of bacilli only at the beginning and at the end of the experimental period. Thalidomide administration did not affect the microbiological or histological features of the infection. Thalidomide-treated and untreated animals showed the same amount of M. avium colonies 3 weeks after infection. Although it did not affect bacillary clearance, thalidomide administration resulted in a decreased percent of spread cells and release of hydrogen peroxide, suggesting that factors other than TNF-a play a role in the killing of mycobacteria by alveolar macrophages. Thalidomide administration also reduced the number of spread cells among resident macrophages, suggesting a direct effect of the drug on this phenomenon

Epidemiología de enfermedades fúngicas de postcosecha en frutos de papaya / Epidemiology of postharcest fungal diseases in papaya fruits

Se analizó el impacto de las enfermedades fúngicas en las pérdidas de postcosecha de papaya en la fase de comercialización, evaluando la influencia de la temperatura en la manifestación de las enfermedades de postcosecha y del binomio temperatura-humedad relativa en la severidad de la pudrición causada por Lasiodiplodia theobromae. Cuatro supermercados, en la cuidad de Recife (Estado de Pernambuco, Brasil), fueron analizados en cuanto al impacto de las enfermedades fúngicas postcosecha en las pérdidas de frutos de papaya. Estas fueron las principales causas de descarte, correspondiendo un 98 por ciento al Cultivar Formosa y 90,5 por ciento al Cultivar Sunrise Solo. Los principales hongos involucrados en el proceso de infección fueron Colletotrichum gloeosporiodes, L. Theobromae y Rhizopus stolonifer, agentes causales de la antracnosis, pudrición peduncular y pudrición acuosa, respectivamente. Al analizar la influencia de la temperatura (5, 15 y 30°C) en los frutos con aspecto sano, se constató que las principales enfermedades fueron las mismas observadas en los frutos descartados. A temperaturas de 5 y 15°C, la antracnosis presentó una mayor incidencia en relación con las otras enfermedades, mientras que la pudrición peduncular fue constatada solamente a 30°C, con incidencia superior a las demás. En el análisis de la influencia de la temperatura y la humedad sobre la severidad de la pudrición causada por L. theobromae, no se observaron síntomas de la enfermedad en frutos en humedad relativa inferior o igual al 90 por ciento. En presencia de elevada humedad, sólo a partir de 25°C fue constada la incidencia de la enfermedad, con un aumento en la severidad de la misma con la elevación de la temperatura y del período de humedad relativa.

Hongos fitopatógenos asociados a frutos comercializados en Recife, Pernambuco, Brasil / Phytopathogenic fungi associated with fruits marketed in Recife, Pernambuco, Brazil

En la central de abastecimiento y en tres ferias libres de la ciudad de Recife, PE(Brasil), se analizaron cada tres meses, durante un año, frutos de aguacate (palta), limón, maracuyá-amarillo, pimentón y tomate para detectar la presencia de enfermedades fúngicas. En cada muestra fueron evaluadas 20 unidades de cada tipo de fruto, en cinco puntos diferentes por sitio de colecta, totalizándo 100 frutos/punto de comercialización, la incidencia de enfermedades fúngicas en los frutos de aguacate fue de 38,52 porciento, en maracuyá de 15,48 porciento, en limón de 17,55 porciento, en pimentón de 1,58 porciento y en tomate de 6,5 porciento. El hongo detectado con mayor frecuencia en los frutos de aguacate fue sphaceloma perseae (20,0 porciento), en limón sphaceloma fawcetti (6,2 porciento), en maracuja cladosporium herbarum (10,3 porciento) en pimentón alternaria alternata (0,7 porciento) y en tomate geotrichum candidum (3,2 porciento). Los altos valores de incidencia de enfermedades fúngicas detectados, indican la necesidad de adoptar procedimientos antes y después de la cosecha, para reducir los riesgos de infección, lo que promovería la reducción significativa de pérdidas de postcosecha