ABSTRACT
STATEMENT OF PROBLEM: Dental implants obtained by additive manufacturing may present changes in the microbiome formed. However, studies profiling the microbial communities formed on Ti-6Al-4V are lacking. PURPOSE: The purpose of this in situ study was to characterize the profile of the microbial communities formed on Ti-6Al-4V disks produced by additive manufacturing and machining. MATERIAL AND METHODS: Titanium disks produced by additive manufacturing (AMD) and machining (UD) were housed in the buccal region of removable intraoral devices. These devices containing both disks were used by eight participants for 96 hours. After every 24 hours of intraoral exposure, the biofilm that had formed on the disks was collected. The 16S rRNA genes from each specimen were amplified and sequenced with the Miseq Illumina instrument and analyzed. Total microbial quantification was evaluated by analysis of variance-type statistics using the nparLD package. The Wilcoxon test was used to evaluate alpha diversity (α=.05). RESULTS: A difference was found in the microbial communities formed on additively manufactured and machined disks, with a reduction in operational taxonomic units (OTUs) for the AMD group compared with the UD group. Firmicutes and Proteobacteria were the most abundant phyla. Of the 1256 genera sequenced, Streptococcus predominated on both disks. CONCLUSIONS: The microbiome of the biofilm formed on the Ti-6Al-4V disks was significantly influenced by the fabrication method. The AMD disks showed lower total microbial counts than the UD disks.
ABSTRACT
STATEMENT OF PROBLEM: Placement of dental implants depends, among other factors, on anatomic conditions such as sufficient bone height and thickness. Thus, individualized dental implants seem to offer benefits for patients with alveolar bone resorption. Additive manufacturing has allowed for the fabrication of custom implants with microscale resolution and, although the efficiency of the process is unclear, is a potential process for manufacturing dental implants. PURPOSE: The purpose of this systematic review was to evaluate the current situation of additive manufacturing techniques for fabricating dental implants. MATERIAL AND METHODS: An electronic search was performed in the databases PubMed, Lilacs, Cochrane Library, and Science Direct, with the terms "additive manufacturing" AND "dental implants," "rapid prototyping" AND "dental implants," "3 D printing" AND "dental implants," "electron beam melting" AND "dental implants," "selective laser melting" AND "dental implants." The articles were screened, and the final selection of articles was obtained by using the inclusion and exclusion criteria. RESULTS: The database search resulted in 1322 articles, which were screened for title and/or summary according to the inclusion criteria. From the selected 38 articles, 30 remained after applying the exclusion criteria. These were read completely, resulting in a selection of 13 articles for this systematic review. Owing to the great variety of articles with different objectives, the results were based on a descriptive analysis of the following topics: additive manufacturing technique and material, printed structure and implant design, implant characteristics, mechanical analysis, surface treatment, and osseointegration. CONCLUSIONS: Additive manufacturing is a new technology that may solve many problems in diverse fields. In dentistry, however, further studies are needed to improve the method for manufacturing custom dental implants because no standard methodology is available. Moreover, the advantages and disadvantages of the process are not yet clearly defined.
