A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)

The ability to successfully identify and incriminate pathogen vectors is fundamental to effective pathogen control and management. This task is confounded by the existence of cryptic species complexes.Molecular markers can offer a highly effective means of species identification in such complexes and are routinely employed in the study of medical entomology. Here we evaluate a multi-locus system for the identification of potential malaria vectors in the Anopheles strodei subgroup. Methods: Larvae, pupae and adult mosquitoes (n = 61) from the An. strodei subgroup were collected from 21 localities in nine Brazilian states and sequenced for the COI, ITS2 and white gene. A Bayesian phylogenetic approach was used to describe the relationships in the Strodei Subgroup and the utility of COI and ITS2 barcodes was assessed using the neighbor joining tree and “best close match” approaches...

Valoración del daño corporal en amputados de miembros inferiores: prueba de sensibilidad, postura, sobrecarga articular y calidad de vida / Assessment of body damage in subjects with lower-limb amputation: sensitivity test, posture, joint overload and quality of life

Objetivo: Evaluar de forma integral a pacientes amputados de extremidades inferiores, valorando su daño corporal y desarrollar informes objetivos y conclusivos de las pruebas. Material y metodología: Se evaluan veintiocho amputados adultos de ambos sexos, con amputación de cualquier nivel y etiología, muñón estable y utilización de prótesis con marcha independiente. Se estudia la sensibilidad del muñón, temperatura, la marcha y vicios posturales y la calidad de vida de estas personas. La sensibilidad del muñón y miembro inferior se valora a través de los monofilamentos Semmes-Weinstein y la postura de los sujetos con el software SAPO. La calidad de vida fue valorada a través del cuestionario de la Organización Mundial de la Salud, WHOQOL breef, y la temperatura fue obtenida con termografía utilizando una cámara infrarroja. Resultados: Obtuvimos baja calidad de vida física y psicológica, alteraciones posturales en las extremidades inferiores, alteración o pérdida de la sensibilidad y altas temperaturas en la extremidad del muñón de la amputación con sobrecarga en la marcha. Conclusión: El amputado de extremidad inferior tiene una baja calidad de vida física y psicológica, mayores alteraciones posturales en las extremidades inferiores, alteración o pérdida de la sensibilidad y altas temperaturas en la extremidad del muñón de la amputación y sobrecarga durante la marcha (AU)

Objective: To evaluate comprehensively subjects with lower-limb amputation, assessing their body damage, and draw up objective, conclusive reports on the tests. Material and method: Twenty-eight adult male and female amputees, with amputation at any site and aetiology, with stable stump and use of prosthesis with independent walking, were evaluated. The sensitivity of the stump, temperature, gait, postural defects and quality of life of these subjects were studied. Sensitivity of the stump and lower limb were assessed through Semmes-Weinstein monofilaments. The posture of the subjects was evaluated with the SAPO software. Quality of life was assessed through the WHOQUOL breef questionnaire and temperature was obtained by thermography using an infrared chamber. Results:We obtained a low physical and psychological quality of life, postural disorders in the lower limbs, impairment or loss of sensitivity and high temperatures in the edge of the amputation stump, with overload on walking. Conclusion: The subject with amputated lower limb has a low physical and psychological quality of life, postural disorders in the lower limbs, impairment or loss of sensitivity and high temperatures in the edge of the amputation stump, with overload on walking (AU)