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1.
Sex Transm Infect ; 82(4): 311-6, 2006 Aug.
Article in English | MEDLINE | ID: mdl-16877581

ABSTRACT

OBJECTIVE: Assessment of HIV prevalence and associated risk behaviours among female commercial sex workers (FCSW) across major cities in South America. METHODS: Seroepidemiological, cross sectional studies of 13 600 FCSW were conducted in nine countries of South America during the years 1999-2002. Participants were recruited in brothels, massage parlours, hotels, and streets where anonymous questionnaires and blood samples were collected. HIV infection was determined by enzyme linked immunosorbent assay (ELISA) screening and western blot confirmatory tests. RESULTS: The overall HIV seroprevalence was 1.2% (range 0.0%-4.5%). The highest HIV seroprevalences were reported in Argentina (4.5%) and Paraguay (2.6%); no HIV infected FCSW were detected in Venezuela and Chile. Consistent predictors of HIV seropositivity were: (1) a previous history of sexually transmitted infections (STI, AORs = 3.8-8.3), and (2) 10 years or more in commercial sex work (AORs = 2.2-24.8). In addition, multiple (> or =3) sexual contacts (AOR = 5.0), sex with foreigners (AOR = 6.9), use of illegal drugs (AOR = 3.2), and marijuana use (AOR = 8.2) were associated with HIV seropositivity in Southern Cone countries. CONCLUSIONS: Consistently low HIV seroprevalences were detected among FCSW in South America, particularly in the Andean region. Predictors of HIV infection across the continent were STI and length of commercial sex work; however, use of illegal drugs, especially marijuana, and sexual contacts with foreigners were also found to be associated risk factors in the Southern Cone region. Interventions for the control of HIV and other STI need to be region and country specific; drug use appears to have an ever increasing role in the spread of HIV among heterosexually active populations.


Subject(s)
HIV Infections/epidemiology , HIV Seroprevalence , HIV-1 , Sex Work/statistics & numerical data , Adult , Blotting, Western , Enzyme-Linked Immunosorbent Assay , Epidemiologic Methods , Female , Humans , Risk Factors , South America/epidemiology
2.
Ann Trop Med Parasitol ; 100 Suppl 1: S73-S86, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16630393

ABSTRACT

Programmes for the surveillance of Aedes aegypti (L.) often focus on residential areas, ignoring non-residential sites. Between November 2003 and October 2004, pupal/demographic surveys were therefore conducted in non-residential sites in the Peruvian city of Iquitos. The sampled sites included schools, factories, ports, public markets, petrol stations, commercial zones, airports, government buildings, animal-production areas, and recreational areas. Compared with the residential sites that had been surveyed a few years earlier, the non-residential sites generally had fewer pupae/ha, even though pupae were found in a high percentage of the sites investigated. Nonetheless, although <56 pupae/ha were observed in the industrial, commercial, recreational and school sites, the river boats in the ports and the areas in and around public markets sometimes had pupal abundances (of 122-213 pupae/ha) that were comparable with those previously recorded in the residential sites. When the relative production of Ae. aegypti was calculated by container type and characteristic (lidded/lidless, indoors/outdoors, and water-use patterns), no single container category was found to be a major producer of Ae. aegypti, with the exception of flower vases in cemeteries. In general, almost all (97%) of the pupae collected in the non-residential sites came from unlidded containers, although 91% of those collected in river boats came from lidded storage areas. With the exception of lumber mills, plant nurseries and markets (where only 39%-60% of the pupae were collected outdoors), >70% of pupal production was outdoors. In commercial areas, 41% of the pupae came from manually-filled containers, compared with <12% in residential sites. These results indicate that non-residential sites can be highly productive for Ae. aegypti and that the role of such sites in dengue transmission requires further investigation.


Subject(s)
Aedes , Dengue , Insect Vectors , Animals , Cities , Dengue/epidemiology , Dengue/prevention & control , Dengue/transmission , Household Articles , Humans , Peru/epidemiology , Population Surveillance/methods , Pupa , Urban Health , Water Supply
3.
J Clin Virol ; 34 Suppl 2: S61-8, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16461242

ABSTRACT

BACKGROUND: Transfusion-transmitted infections (TTIs) constitute a major health problem worldwide where routine screening of blood or blood products is improperly done, and where non-medical injecting medications and/or drug use are prevalent. Prevalence and risk factors vary by geographic location and by the specific TTI (including HIV-1, HBV, HCV and HTLV-I). OBJECTIVE: To determine the prevalence and risk factors associated with TTIs among a sample of multi-transfused adult patients in Peru. STUDY DESIGN: A cross-sectional multi-center study was conducted across seven major hospitals in Peru from February 2003 to September 2004. Self-reported behavior information (medical procedures, number of sexual partners, and drug use history) was analyzed, along with a review of exposure history from hospital medical records. Prevalences were calculated by TTI for different exposures, along with unadjusted and adjusted odds ratios for infection risk. RESULTS: Overall, 192 (54.7%) of 351 multi-transfused patients were found infected with one or more TTIs. Number of transfusion units, years of transfusion history (6 or more), and number of treatment facilities (2 or more) were associated with HCV infection. Hemodialysis history was a common risk factor associated with HBV, HCV and HTLV-I infection. HIV infection was associated only with total number of transfusion units received. CONCLUSIONS: High prevalences of HBV and HCV infection were found among Peruvian multi-transfused patients and were associated with a past history and number of blood transfusions, as well as with past hemodialysis procedures. TTIs continue to represent a significant public health problem in Peru. Continued vigilant attention to blood safety procedures, including universal screening and health care provider education, is recommended.


Subject(s)
Antibodies, Viral/blood , HIV Antibodies/blood , HIV Infections/epidemiology , HTLV-I Infections/epidemiology , Hepatitis B Antibodies/blood , Hepatitis B/epidemiology , Hepatitis C Antibodies/blood , Hepatitis C/epidemiology , Human T-lymphotropic virus 1/immunology , Renal Dialysis , Transfusion Reaction , Adolescent , Adult , Cross-Sectional Studies , Disease Transmission, Infectious , Female , HIV Infections/transmission , HTLV-I Infections/transmission , Hepatitis B/transmission , Hepatitis C/transmission , Hospitals , Humans , Male , Middle Aged , Peru/epidemiology , Risk Factors
4.
Sex Transm Infect ; 80(6): 498-504, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15572623

ABSTRACT

OBJECTIVES: Sex among men constitutes an important route of transmission for HIV type 1 (HIV-1) in Latin America. Seeking better understanding of risk behaviours in this region, we determined the seroprevalence, potential risk factors, and geographic distribution of HIV-1 among groups of men who have sex with men (MSM). METHODS: Seroepidemiological, cross sectional studies of 13,847 MSM were conducted in seven countries of South America during the years 1999-2002. Volunteers were recruited in city venues and streets where anonymous questionnaires and blood samples were obtained. HIV-1 infection was determined by enzyme linked immunosorbent assay (ELISA) screening and western blot (WB) confirmatory tests. RESULTS: HIV-1 seroprevalence varied widely (overall 12.3%, range 11.0%-20.6%). The highest HIV-1 seroprevalence was noted in Bolivia (20.6%) and the lowest in Peru (11.0%). Predictors of HIV-1 infection varied among countries; however, a history of previous sexually transmitted disease (STD) was associated with a consistent increased risk (ORs=1.9-2.9, AORs=1.8-2.7). Multiple weekly sexual contacts was found to represent a secondary risk factor in Ecuador, Peru, and Argentina (ORs=1.6-2.9, AORs=1.6-3.1), whereas use of drugs such as cocaine was found to increase risk in Bolivia, Uruguay, and Paraguay (ORs=2.5-6.5, AORs=2.6-6.1). CONCLUSION: The results of this study illustrate an elevated HIV-1 seroprevalence among MSM participants from Andean countries. A previous STD history and multiple partners predicted HIV-1 infection in the seven countries of South America. In Southern Cone countries, HIV-1 infection was also associated with use of illegal drugs such as cocaine.


Subject(s)
HIV Infections/epidemiology , HIV Seroprevalence , HIV-1 , Homosexuality, Male/statistics & numerical data , Adolescent , Adult , Aged , Humans , Male , Middle Aged , Multivariate Analysis , Risk Factors , Sexual Partners , South America/epidemiology , Substance-Related Disorders/epidemiology , Unsafe Sex/statistics & numerical data , Urban Health
5.
AIDS Res Hum Retroviruses ; 20(9): 1022-5, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15585091

ABSTRACT

HIV subtypes B, F, and BF recombinants have been previously reported in South America. This report describes the presence of HIV-1 subtype C infection in the countries of Argentina, Uruguay, and Paraguay dating back to at least 1999. Surveillance for uncommon non-B/non-F subtype viruses circulating in South America has been conducted in samples obtained from nine countries. Peripheral blood mononuclear cells (PBMC), dried filter paper (FP), and fresh blood (FB) samples were collected from HIV-positive patients from Ecuador, Colombia, Venezuela, Peru, Chile, Bolivia, Argentina, Uruguay, and Paraguay. From a total of 2962 HIV seropositive samples examined during a 9-year period (1995-2003), only 11 (0.4%) were found to be infected with non-B/non-F HIV variants. Eight of these 11 strains were determined to be subtype C by heteroduplex mobility assay (HMA). Five of these 8 strains were further characterized by sequencing and phylogenetic analysis of the protease (Pro) and reverse transcriptase (RT) region of the genome and two were sequenced full length. One of the strains was found to be a unique BC recombinant. The spread of a third subtype of HIV, subtype C, should raise the question of its potential future role in the HIV epidemic in this region.


Subject(s)
HIV Infections/epidemiology , HIV Infections/virology , HIV-1/classification , HIV-1/genetics , Adult , Argentina/epidemiology , Female , HIV Protease/genetics , HIV Reverse Transcriptase/genetics , Heteroduplex Analysis , Humans , Male , Middle Aged , Molecular Sequence Data , Paraguay/epidemiology , Phylogeny , Sequence Analysis, DNA , Uruguay/epidemiology
6.
J Gen Virol ; 84(Pt 10): 2685-2689, 2003 Oct.
Article in English | MEDLINE | ID: mdl-13679602

ABSTRACT

A virus isolated from dead Chaerephon plicata bats collected near Kampot, Cambodia, was identified as a member of the family Bunyaviridae by electron microscopy. The only bunyavirus previously isolated from Chaerephon species bats in South-East Asia is Kaeng Khoi (KK) virus (genus Orthobunyavirus), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian C. plicata bats were found to be almost identical and to differ from those of the prototype KK virus by only 2.6-3.2 %, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus Orthobunyavirus.


Subject(s)
Bunyaviridae Infections/veterinary , Bunyaviridae/classification , Bunyaviridae/isolation & purification , Chiroptera/virology , Animals , Brain/virology , Bunyaviridae/genetics , Bunyaviridae/pathogenicity , Bunyaviridae Infections/virology , Cambodia , Chlorocebus aethiops , Mice , Phylogeny , RNA, Viral/analysis , Reverse Transcriptase Polymerase Chain Reaction , Vero Cells
7.
Can J Public Health ; 92(1): 67-71, 2001.
Article in English | MEDLINE | ID: mdl-11257996

ABSTRACT

A survey for antibodies against agents of plague, tularemia, and Rocky Mountain spotted fever (RMSF), and against Sin Nombre hantavirus (SNV), Bartonella henselae and B. clarridgeiae was conducted in the summer of 1995 using serum from rural dogs and cats living in the vicinity of four public parks in southeastern Alberta and southwestern Saskatchewan. Antibodies to all pathogens were detected in all survey areas. Overall prevalence rates were 0.075 for Yersinia pestis, 0.089 for Francisella tularensis, 0.025 for Rickettsia rickettsii (dogs only), and 0.029, 0.178 and 0.186 for SNV, B. henselae and B. clarridgeiae, respectively (cats only). This serological survey of rural dogs and cats was more sensitive and efficient than previous surveys based on collection and culture of rodents and ectoparasites. All six pathogens appear endemic to the region. Surveillance for plague, tularemia, RMSF and SNV, and management of associated public risks should be done in endemic regions.


Subject(s)
Cat Diseases/epidemiology , Dog Diseases/epidemiology , Seroepidemiologic Studies , Zoonoses/epidemiology , Animals , Blood-Borne Pathogens , Canada/epidemiology , Cat Diseases/diagnosis , Cat Diseases/microbiology , Cats , Dog Diseases/diagnosis , Dog Diseases/microbiology , Dogs , Zoonoses/microbiology
8.
Vector Borne Zoonotic Dis ; 1(2): 119-27, 2001.
Article in English | MEDLINE | ID: mdl-12653142

ABSTRACT

A retrospective cohort study was conducted among troops training at Fort Chaffee, Arkansas, from May through June 1997, to identify infections caused by tick-borne pathogens. Serum samples were tested by IFAs for antibodies to selected Rickettsia and Ehrlichia species and by an investigational EIA for spotted fever group Rickettsia lipopolysaccharide antigens. Of 1,067 guardsmen tested, 162 (15.2%) had antibodies to one or more pathogens. Of 93 guardsmen with paired serum samples, 33 seroconverted to Rickettsia rickettsii or spotted fever group rickettsiae (SFGR) and five to Ehrlichia species. Most (84.8%) of the personnel who seroconverted to SFGR were detected only by EIA, and seropositivity was significantly associated with an illness compatible with a tick-borne disease. In addition, 34 (27%) of 126 subjects with detectable antibody titers reported a compatible illness. The primary risk factor for confirmed or probable disease was finding > 10 ticks on the body. Doxycycline use and rolling up of long sleeves were protective against seropositivity. The risk of transmission of tick-borne pathogens at Fort Chaffee remains high, and use of the broadly reactive EIA suggests that previous investigations may have underestimated the risk for infection by SFGR. Measures to prevent tick bite and associated disease may require reevaluation.


Subject(s)
Ehrlichiosis/epidemiology , Military Personnel , Rickettsia Infections/epidemiology , Tick-Borne Diseases/epidemiology , Ticks/microbiology , Adolescent , Adult , Animals , Anti-Bacterial Agents/therapeutic use , Antibodies, Bacterial/analysis , Arachnid Vectors/microbiology , Arkansas/epidemiology , Clothing , Cohort Studies , Doxycycline/therapeutic use , Ehrlichia/immunology , Ehrlichia/isolation & purification , Ehrlichiosis/prevention & control , Ehrlichiosis/transmission , Female , Humans , Male , Middle Aged , Military Medicine , Rickettsia/immunology , Rickettsia/isolation & purification , Rickettsia Infections/prevention & control , Rickettsia Infections/transmission , Risk Factors , Tick Control , Tick-Borne Diseases/prevention & control , Tick-Borne Diseases/transmission
9.
J Am Vet Med Assoc ; 215(9): 1276-80, 1999 Nov 01.
Article in English | MEDLINE | ID: mdl-10553437

ABSTRACT

Substantial changes in the epizootic characteristics of rabies have transpired in the United States during the past 50 years. Traditional veterinary practices and public health recommendations have effectively controlled rabies in dogs and prevented associated human fatalities; however, they have been unable to adequately address the problem of rabies in wildlife. Attributable in part to a renewed focus on emerging infectious diseases, a conference was held at the Centers for Disease Control and Prevention in 1993 to begin discussion focused on the reemergence of rabies and to formulate new suggestions for prevention and control of rabies in the United States. Three major working groups were formed from a national committee of professionals representing a broad array of biomedical disciplines. These groups concentrated on prevention of rabies in human beings, education, laboratory diagnosis of rabies, and rabies control in animals. The groups described the perceived minimum requirements to promote prevention and control of rabies in the United States into the next century. The following article describes the needs and recommendations identified by the prevention and education working group. Two other articles, scheduled for the Nov 15 and Dec 1, 1999 issues of JAVMA, will relay the needs and recommendations of the working groups on laboratory diagnosis of rabies and rabies in wildlife.


Subject(s)
Animals, Wild , Rabies/prevention & control , Zoonoses , Animals , Humans , Rabies/epidemiology , United States/epidemiology
10.
Transfusion ; 39(8): 828-33, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10504117

ABSTRACT

BACKGROUND: Tick-borne illnesses were diagnosed in a group of National Guard members, including some who had donated blood a few days before the onset of symptoms. A voluntary recall of those blood components was issued and a multistate investigation was conducted to determine if transfusion-transmitted illness had occurred. STUDY DESIGN AND METHODS: Donors and recipients were asked to complete questionnaires regarding symptoms and risk factors for infection and to provide blood samples for laboratory analysis. RESULTS: Among National Guard personnel who donated blood, 12 individuals were found to have a confirmed or probable case of Rocky Mountain spotted fever or ehrlichiosis. A total of 320 units (platelets or packed red cells) from 377 donors were transfused into 129 recipients. Although 10 recipients received units from National Guard personnel with confirmed or probable infection, none became ill. CONCLUSION: Transfusion-transmitted illness did not occur. Despite the awareness of the risk for tick-borne diseases and the use of tick-preventive measures, many National Guard personnel reported exposure to ticks. In addition to augmenting current tick-preventive measures, scheduling blood drives before rather than after field exercises could further reduce the potential for transmission of tick-borne pathogens.


Subject(s)
Rocky Mountain Spotted Fever/transmission , Transfusion Reaction , Adult , Blood Donors , Blood Platelets/microbiology , Erythrocytes/microbiology , Humans , Middle Aged , Military Personnel
11.
Am J Trop Med Hyg ; 61(2): 344-9, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10463692

ABSTRACT

During May 1998, we conducted a case-control study of 357 participants from 60 households during an outbreak of acute bartonellosis in the Urubamba Valley, Peru, a region not previously considered endemic for this disease. Blood and insect specimens were collected and environmental assessments were done. Case-patients (n = 22) were defined by fever, anemia, and intra-erythrocytic coccobacilli seen in thin smears. Most case-patients were children (median age = 6.5 years). Case-patients more frequently reported sand fly bites than individuals of neighboring households (odds ratio [OR] = 5.8, 95% confidence interval [CI] = 1.2-39.2), or members from randomly selected households > or = 5 km away (OR = 8.5, 95% CI = 1.7-57.9). Bartonella bacilliformis isolated from blood was confirmed by nucleotide sequencing (citrate synthase [g/tA], 338 basepairs). Using bacterial isolation (n = 141) as the standard, sensitivity, specificity, and positive predictive value of thin smears were 36%, 96%, and 44%, respectively. Patients with clinical syndromes compatible with bartonellosis should be treated with appropriate antibiotics regardless of thin-smear results.


Subject(s)
Bartonella Infections/epidemiology , Bartonella/isolation & purification , Disease Outbreaks , Adolescent , Adult , Bartonella Infections/diagnosis , Bartonella Infections/physiopathology , Case-Control Studies , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Peru/epidemiology , Risk Factors
12.
Am J Trop Med Hyg ; 61(2): 350-4, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10463693

ABSTRACT

Between November 1993 and March 1994, a cluster 6 pediatric patients with acute febrile illnesses associated with rashes was identified in Jujuy Province, Argentina. Immunohistochemical staining of tissues confirmed spotted fever group rickettsial infection in a patient with fatal disease, and testing of serum of a patient convalescing from the illness by using an indirect immunofluorescence assay (IFA) demonstrated antibodies reactive with spotted fever group rickettsiae. A serosurvey was conducted among 16 households in proximity to the index case. Of 105 healthy subjects evaluated by IFA, 19 (18%) demonstrated antibodies reactive with rickettsiae or ehrlichiae: 4 had antibodies reactive with Rickettsia rickettsii, 15 with Ehrlichia chaffeensis, and 1 with R. typhi. Amblyomma cajennense, a known vector of R. rickettsii in South America, was collected from pets and horses in the area. These results are the first to document rickettsial spotted fever and ehrlichial infections in Argentina.


Subject(s)
Ehrlichia chaffeensis/isolation & purification , Ehrlichiosis/epidemiology , Rickettsia rickettsii/isolation & purification , Rocky Mountain Spotted Fever/epidemiology , Adolescent , Adult , Antibodies, Bacterial/blood , Argentina/epidemiology , Child , Child, Preschool , Ehrlichia chaffeensis/immunology , Fatal Outcome , Female , Fluorescent Antibody Technique , Humans , Immunoenzyme Techniques , Male , Rickettsia rickettsii/immunology , Rocky Mountain Spotted Fever/physiopathology , Seroepidemiologic Studies
13.
J Infect Dis ; 179(6): 1469-76, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10228069

ABSTRACT

Rocky Mountain spotted fever (RMSF) is the most severe tickborne infection in the United States and is a nationally notifiable disease. Since 1981, the annual case-fatality ratio for RMSF has been determined from laboratory-confirmed cases reported to the Centers for Disease Control and Prevention (CDC). Herein, a description is given of patients with fatal, serologically unconfirmed RMSF for whom a diagnosis of RMSF was established by immunohistochemical (IHC) staining of tissues obtained at autopsy. During 1996-1997, acute-phase serum and tissue samples from patients with fatal disease compatible with RMSF were tested at the CDC. As determined by indirect immunofluorescence assay, no patient serum demonstrated IgG or IgM antibodies reactive with Rickettsia rickettsii at a diagnostic titer (i.e., >/=64); however, IHC staining confirmed diagnosis of RMSF in all patients. Polymerase chain reaction validated the IHC findings for 2 patients for whom appropriate samples were available for testing. These findings suggest that dependence on serologic assays and limited use of IHC staining for confirmation of fatal RMSF results in underestimates of mortality and of case-fatality ratios for this disease.


Subject(s)
Rocky Mountain Spotted Fever/diagnosis , Rocky Mountain Spotted Fever/mortality , Adult , Aged , Centers for Disease Control and Prevention, U.S. , Child, Preschool , Disease Notification , Female , Humans , Immunohistochemistry , Infant , Male , Middle Aged , Population Surveillance , Rickettsia rickettsii/isolation & purification , Rocky Mountain Spotted Fever/blood , Rocky Mountain Spotted Fever/epidemiology , United States
14.
J Clin Microbiol ; 37(3): 558-64, 1999 Mar.
Article in English | MEDLINE | ID: mdl-9986812

ABSTRACT

An indirect immunofluorescence assay (IFA) was used to identify patients with antibodies reactive to the human granulocytic ehrlichiosis (HGE) agent. Serum samples collected from clinically ill individuals were submitted to the Centers for Disease Control and Prevention by physicians via state health departments from throughout the United States and tested against a panel of ehrlichial and rickettsial pathogens. Antibodies reactive to the HGE agent were detected in 142 (8.9%) of 1,602 individuals tested. There were 19 confirmed and 59 probable (n = 78) cases of HGE as defined by seroconversion or a fourfold or higher titer to the HGE agent than to the Ehrlichia chaffeensis antigens. The average age of patients with HGE was 57 years, and males accounted for 53 (68%) of the patients. Cases of HGE occurred in 21 states; 47 (60%) of the cases occurred in Connecticut (n = 14), New York (n = 18), and Wisconsin (n = 15). Onset of HGE was identified from April through December, with cases peaking in June and July. The earliest confirmed cases of HGE occurred in 1987 in Wisconsin and 1988 in Florida. No fatalities were reported among the 78 patients with confirmed or probable HGE. Reactivity to the HGE agent and to either Coxiella burnetii, Rickettsia rickettsii, or Rickettsia typhi was infrequent; however, 74 (52%) of the 142 individuals who were positive for HGE had at least one serum sample that also reacted to the E. chaffeensis antigen. Thirty-four persons with confirmed or probable human monocytic ehrlichiosis due to E. chaffeensis also had antibodies to the HGE agent in at least one serum sample. The specific etiologic agent for 30 patients was not ascribed because of similarity of titers to both ehrlichial antigens. The use of both antigens may be required to correctly diagnose most cases of human ehrlichiosis, especially in geographic regions where both the HGE agent and E. chaffeensis occur.


Subject(s)
Antibodies, Bacterial/blood , Ehrlichia chaffeensis , Ehrlichiosis/diagnosis , Adolescent , Adult , Aged , Aged, 80 and over , Antigens, Bacterial/immunology , Centers for Disease Control and Prevention, U.S. , Child , Child, Preschool , Demography , Ehrlichia chaffeensis/immunology , Ehrlichiosis/epidemiology , Ehrlichiosis/immunology , Female , Granulocytes/immunology , Humans , Male , Middle Aged , Prevalence , Seasons , Serologic Tests , United States/epidemiology
15.
Clin Diagn Lab Immunol ; 6(1): 41-4, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9874661

ABSTRACT

We studied evidence of Bartonella henselae and Bartonella clarridgeiae infection in 54 cats living in Jakarta, Indonesia. By using an indirect immunofluorescence assay, we found immunoglobulin G antibody to B. henselae in 40 of 74 cats (54%). The blood of 14 feral cats was cultured on rabbit blood agar plates for 28 days. Bartonella-like colonies were identified as B. henselae or B. clarridgeiae by using restriction fragment length polymorphism analysis and direct sequencing of the PCR amplicons. Of the cats sampled in the study, 6 of 14 (43%; all feral) were culture positive for B. henselae; 3 of 14 (21%; 2 feral and 1 pet) culture positive for B. clarridgeiae. This is the first report that documents B. henselae and B. clarridgeiae infections in Indonesian cats.


Subject(s)
Bartonella Infections/veterinary , Bartonella henselae , Cat Diseases/epidemiology , Animals , Antibodies, Bacterial/blood , Bartonella/genetics , Bartonella/immunology , Bartonella/isolation & purification , Bartonella Infections/epidemiology , Bartonella Infections/immunology , Bartonella henselae/genetics , Bartonella henselae/immunology , Bartonella henselae/isolation & purification , Base Sequence , Cat Diseases/immunology , Cat Diseases/microbiology , Cat-Scratch Disease/transmission , Cats , DNA Primers/genetics , Disease Reservoirs/veterinary , Female , Fluorescent Antibody Technique, Indirect , Immunoglobulin G/blood , Indonesia/epidemiology , Male , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Rabbits
16.
J Clin Microbiol ; 37(1): 31-4, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9854059

ABSTRACT

A PCR assay of 43 acute-phase serum samples was evaluated as a method for early detection of human granulocytic ehrlichiosis (HGE) and determination of etiology when serologic testing is inconclusive. Sequence-confirmed products of the HGE agent were amplified from three individuals residing or having exposure history in Minnesota or Wisconsin, and similarly confirmed products from Ehrlichia chaffeensis were amplified from three individuals from Florida or Maryland. Etiology, as determined by PCR and serology, was the same whenever there was a fourfold difference between the maximum titers of antibodies to both antigens, indicating that presumptive determination of etiology may be based on fourfold differences in titers. PCR testing determined that E. chaffeensis was the etiologic agent for one individual who had similar titers of antibodies to both agents. PCR assay of acute-phase serum in the absence of whole blood specimens may be a useful method for early detection of human ehrlichiosis and determination of etiology when serologic testing is inconclusive.


Subject(s)
Ehrlichia chaffeensis/isolation & purification , Ehrlichiosis/diagnosis , Polymerase Chain Reaction/methods , Acute-Phase Reaction/blood , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Ehrlichia chaffeensis/genetics , Ehrlichiosis/blood , Humans , RNA, Ribosomal, 16S/analysis , RNA, Ribosomal, 16S/genetics
17.
Ann Intern Med ; 128(11): 922-30, 1998 Jun 01.
Article in English | MEDLINE | ID: mdl-9634432

ABSTRACT

PURPOSE: To summarize the epidemiologic, diagnostic, and clinical features of the 32 laboratory-confirmed cases of human rabies diagnosed in the United States from 1980 to 1996. DATA SOURCES: Data were obtained from case reports of human rabies submitted to the Centers for Disease Control and Prevention by state or local health authorities. STUDY SELECTION: All cases of human rabies reported in the United States from 1980 to 1996 in which infection with rabies virus was confirmed by laboratory studies. DATA EXTRACTION: Patients were reviewed for demographic characteristics, exposure history, rabies prophylaxis, clinical presentation, treatment, clinical course, diagnostic laboratory tests, identification of rabies virus variants, and the number of medical personnel or family members who required postexposure prophylaxis after coming in contact with an exposed person. DATA SYNTHESIS: 32 cases of human rabies were reported from 20 states. Patients ranged in age from 4 to 82 years and were predominantly male (63%). Most patients (25 of 32) had no definite history of an animal bite or other event associated with rabies virus transmission. Of the 32 cases, 17 (53%) were associated with rabies virus variants found in insectivorous bats, 12 (38%) with variants found in domestic dogs outside the United States, 2 (6%) with variants found in indigenous domestic dogs, and 1 (3%) with a variant found in indigenous skunks. Among the 7 patients with a definite exposure history, 6 cases were attributable to dog bites received in foreign countries and 1 was attributable to a bat bite received in the United States. In 12 of the 32 patients (38%), rabies was not clinically suspected and was diagnosed after death. In the remaining 20 cases (63%), the diagnosis of rabies was considered before death and samples were obtained specifically for laboratory confirmation a median of 7 days (range, 3 to 17 days) after the onset of clinical signs. Of the clinical differences between patients in whom rabies was diagnosed before death and those in whom it was diagnosed after death, the presence of hydrophobia or aerophobia was significantly associated with antemortem diagnosis (odds ratio, 11.0 [95% CI, 1.05 to 273.34]). The median number of medical personnel or familial contacts of the patients who received postexposure prophylaxis was 54 per patient (range, 4 to 179). None of the 32 patients with rabies received postexposure prophylaxis before the onset of clinical disease. CONCLUSIONS: In the United States, human rabies is rare but probably underdiagnosed. Rabies should be included in the differential diagnosis of any case of acute, rapidly progressing encephalitis, even if the patient does not recall being bitten by an animal. In addition to situations involving an animal bite, a scratch from an animal, or contact of mucous membranes with infectious saliva, postexposure prophylaxis should be considered if the history indicates that a bat was physically present, even if the person is unable to reliably report contact that could have resulted in a bite. Such a situation may arise when a bat bite causes an insignificant wound or the circumstances do not allow recognition of contact, such as when a bat is found in the room of a sleeping person or near a previously unattended child.


Subject(s)
Rabies/epidemiology , Age Distribution , Animals , Diagnosis, Differential , Dog Diseases/epidemiology , Dogs , Female , Humans , Male , Rabies/diagnosis , Rabies/prevention & control , Rabies/transmission , Rabies virus/isolation & purification , United States/epidemiology , Zoonoses/epidemiology , Zoonoses/transmission
18.
J Clin Microbiol ; 36(4): 1090-5, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9542943

ABSTRACT

A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis. Five of the seven suspected cases were positive by the PCR assay using DNA extracted from whole blood as the template, compared with a serologic assay that identified only one positive sample. The PCR assay using DNA extracted from the corresponding serum samples as the template identified three positive samples. The sensitivity of the assay on human samples was examined, and the limit of detection was shown to be fewer than 2 copies of the 16S rRNA gene. The application of the assay to nonhuman samples demonstrated products amplified from template DNA extracted from Ixodes scapularis ticks collected in Rhode Island and from EDTA-blood specimens obtained from white-tailed deer in Maryland. All PCR products were sequenced and identified as specific to granulocytic ehrlichiae. A putative variant granulocytic ehrlichia 16S rRNA gene sequence was detected among products amplified from both the ticks and the deer blood specimens.


Subject(s)
Ehrlichia/isolation & purification , Granulocytes/microbiology , Polymerase Chain Reaction , Animals , DNA, Bacterial/analysis , DNA, Bacterial/chemistry , DNA, Ribosomal/chemistry , Humans , RNA, Ribosomal, 16S/genetics , Sensitivity and Specificity , Ticks/microbiology
19.
Am J Trop Med Hyg ; 57(5): 569-70, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9392597

ABSTRACT

Indonesian peacekeepers in Cambodia provided a unique study population to estimate the threat of rickettsial exposure to Rickettsia typhi (murine typhus), Orientia tsutsugamushi, (scrub typhus), and R. conorii (spotted fever) for the region. Prescreening prevalence measure showed a large proportion (36%) of soldiers with antibodies to R. typhi. Predeployment prevalence for antibodies to O. tsutsugamushi was 8%, with no evidence of background R. conorii infections. Actual seroconversions of R. typhi (3) and O. tsutsugamushi (1), attributed to exposure(s) in Cambodia, translated into annualized incidence rates of 24 and 8 per 1,000 per year, respectively. Surveillance of rickettsial infections and/or disease is particularly warranted in Cambodia with recent recognition of drug-resistant scrub typhus in neighboring Thailand.


Subject(s)
Military Personnel , Rickettsia Infections/epidemiology , Antibodies, Bacterial/blood , Cambodia/epidemiology , Indonesia , Prevalence
20.
Am J Trop Med Hyg ; 57(5): 578-88, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9392599

ABSTRACT

A number of Bartonella isolates were obtained from seven species of rodents sampled from 12 geographic sites representing the major biotic communities of the southeastern United States. Bartonella were isolated from the blood of 42.2% of 279 tested rodents. The highest prevalence of infection typically occurred among the most commonly captured species in the rodent community. Four phylogenetic groups, uniting 14 genotypic variants of Bartonella, were identified by sequence analysis of the citrate synthase gene. The level of sequence homology between genotypic groups varied from 88.8% to 96.4%, and the degree of homology among variants within groups was > or = 97%. Cotton rats (Sigmodon hispidus) harbored up to three phylogenetic groups of Bartonella at a single site, and Bartonella of two phylogenetic groups were isolated from a single rodent. All the Bartonella isolated from three species of Peromyscus clustered in a single distinct phylogenetic group, suggesting some host specificity may occur. Mouse ascitic fluids produced in BALB/c mice inoculated with Bartonella of three phylogenetic groups demonstrated high indirect fluorescent antibody (IFA) titers to homologous antigens. However, use of eight Bartonella antigens in an IFA test with sera from 394 wild-caught rodents resulted in either little or extremely low titers of antibody.


Subject(s)
Bartonella/isolation & purification , Rodentia/microbiology , Animals , Antibodies, Bacterial/blood , Bacteremia/veterinary , Bartonella/classification , Genotype , Mice , Phylogeny , Rats , United States
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