Perfluorobutanoic Acid (PFBA) Induces a Non-Enzymatic Oxidative Stress Response in Soybean (Glycine max L. Merr.).

Short-chain perfluoroalkyl substances (PFAS) are generally considered to be of less environmental concern than long-chain analogues due to their comparatively shorter half-lives in biological systems. Perfluorobutanoic acid (PFBA) is a short-chain PFAS with the most root-shoot transfer factor of all PFAS. We investigated the impact of extended exposure of soybean plants to irrigation water containing environmentally relevant (100 pg-100 ng/L) to high (100 µg-1 mg/L) concentrations of PFBA using phenotypical observation, biochemical characterization, and transcriptomic analysis. The results showed a non-monotonous developmental response from the plants, with maximum stimulation and inhibition at 100 ng/L and 1 mg/L, respectively. Higher reactive oxygen species and low levels of superoxide dismutase (SOD) and catalase (CAT) activity were observed in all treatment groups. However transcriptomic analysis did not demonstrate differential expression of SOD and CAT coding genes, whereas non-enzymatic response genes and pathways were enriched in both groups (100 ng/L and 1 mg/L) with glycine betaine dehydrogenase showing the highest expression. About 18% of similarly downregulated genes in both groups are involved in the ethylene signaling pathway. The circadian rhythm pathway was the only differentially regulated pathway between both groups. We conclude that, similar to long chain PFAS, PFBA induced stress in soybean plants and that the observed hormetic stimulation at 100 ng/L represents an overcompensation response, via the circadian rhythm pathway, to the induced stress.

Influence of Perfluorobutanoic Acid (PFBA) on the Developmental Cycle and Damage Potential of the Beet Armyworm Spodoptera exigua (Hübner) (Insecta: Lepidoptera: Noctuidae).

Perfluorobutanoic acid (PFBA), one of the short-chain replacement perfluoroalkyl substances, has been shown to accumulate in plants. The potential of PFBA to modulate the developmental cycle of the beet armyworm, Spodoptera exigua, a polyphagous pest, was investigated. Second-instar larvae were fed with PFBA-spiked artificial diets and leaves from soybean plants grown with PFBA-spiked irrigation water. Spiked PFBA concentrations were 200 µg/kg for the artificial diet, whereas 405 to 15,190 ng/kg accumulated in the soybean leaves. The larvae fed with the PFBA-spiked diet showed a significant increase in weight gain compared with the controls over a 7-day exposure period. A similar weight gain trend was observed with larvae fed with the PFBA-containing soybean leaves, with the dose-response data fitting into a Brain-Cousens hormesis model with a 57% stimulation over controls. The artificial diet treatments showed 66.7% metamorphosed larva to pupa at 9 days after exposure (dpe) compared with 33.3% of the controls. The adult emergence at 16-dpe followed a similar trend with 57.7% and 33.3%, respectively, for the exposed and control groups. The duration of transition from larvae to adults was more symmetrical and 0.5 day faster for the exposed groups over controls. The beet armyworm caused more damage on leaves from the PFBA exposed plants in a nonmonotonic dose-response manner. The results suggest PFBA may have a stimulatory impact on some hormonal signaling pathways at low doses.