ABSTRACT
INTRODUCTION: Hemogram parameters have been recently proposed as markers of inflammation in various studies from different parts of the world. Two of these hemogram parameters are red cell distribution width (RDW) and mean platelet volume (MPV). AIM: To evaluate the relation between RDW and MPV with disease activity of rheumatoid arthritis. To assess whether RDW and MPV can be used to follow disease activity in RA patients. METHODS: This is an observational cross-sectional study that was carried out on 60 rheumatoid arthritis patients who fulfilled the ACR/EULAR2010 classification criteria of RA attending to Rheumatology and Rehabilitation inpatient and outpatient clinics at Zagazig University Hospitals. All cases were subjected to full history taking, clinical examination, and laboratory investigations; differential complete blood picture (CBC), acute phase reactants (CRP and ESR), rheumatoid factor (RF) and anti-cyclic-citrullinated peptide (anti-CCP) antibodies. Disease activity was measured by disease activity score 28 (DAS28). RESULTS: The cut-off levels of RDW and MPV were 14.85 and 11.25. Patients with RDW>14.85 had higher Disease Activity Score 28 (DAS28; p=0.0003), ESR (p=0.0001) and CRP (p=0.0001). RDW was positively correlated with disease activity markers (ESR, CRP and DAS28) in rheumatoid arthritis patients. But, DAS28 was not different between patients with MPV>11.25 and <11.25. CONCLUSION: RDW was strongly correlated with disease activity. Also, RDW was better than ESR and CRP in detecting RA disease activity. According to these findings we suggest that RDW can be used in clinics to follow disease activity. In addition, RDW is widely available; as it's usually included in routine complete blood picture and there will be no need for further cost.
Subject(s)
Arthritis, Rheumatoid , Mean Platelet Volume , Arthritis, Rheumatoid/diagnosis , C-Reactive Protein/analysis , Cross-Sectional Studies , Erythrocyte Indices , HumansABSTRACT
Introduction: Hemogram parameters have been recently proposed as markers of inflammation in various studies from different parts of the world. Two of these hemogram parameters are red cell distribution width (RDW) and mean platelet volume (MPV). Aim: To evaluate the relation between RDW and MPV with disease activity of rheumatoid arthritis. To assess whether RDW and MPV can be used to follow disease activity in RA patients. Methods: This is an observational cross-sectional study that was carried out on 60 rheumatoid arthritis patients who fulfilled the ACR/EULAR2010 classification criteria of RA attending to Rheumatology and Rehabilitation inpatient and outpatient clinics at Zagazig University Hospitals. All cases were subjected to full history taking, clinical examination, and laboratory investigations; differential complete blood picture (CBC), acute phase reactants (CRP and ESR), rheumatoid factor (RF) and anti-cyclic-citrullinated peptide (anti-CCP) antibodies. Disease activity was measured by disease activity score 28 (DAS28). Results: The cut-off levels of RDW and MPV were 14.85 and 11.25. Patients with RDW>14.85 had higher Disease Activity Score 28 (DAS28; p=0.0003), ESR (p=0.0001) and CRP (p=0.0001). RDW was positively correlated with disease activity markers (ESR, CRP and DAS28) in rheumatoid arthritis patients. But, DAS28 was not different between patients with MPV>11.25 and <11.25. Conclusion: RDW was strongly correlated with disease activity. Also, RDW was better than ESR and CRP in detecting RA disease activity. According to these findings we suggest that RDW can be used in clinics to follow disease activity. In addition, RDW is widely available; as it's usually included in routine complete blood picture and there will be no need for further cost.(AU)
Introducción: Los parámetros del hemograma se han propuesto recientemente como marcadores de inflamación en varios estudios de diferentes partes del mundo. Dos de estos parámetros del hemograma son el ancho de distribución de glóbulos rojos (RDW) y el volumen plaquetario medio (MPV). Objetivo: Evaluar la relación entre RDW y MPV con la actividad de la enfermedad de la artritis reumatoide (AR). Evaluar si RDW y MPV se pueden utilizar para seguir la actividad de la enfermedad en pacientes con AR. Métodos: Se trata de un estudio transversal observacional que se llevó a cabo en 60 pacientes con AR que cumplieron con los criterios de clasificación ACR/EULAR2010 de AR que acudieron a consultas hospitalarias y ambulatorias de reumatología y rehabilitación en los Hospitales Universitarios de Zagazig. Todos los casos fueron sometidos a una anamnesis completa, un examen clínico e investigaciones de laboratorio; imagen diferencial arterial completa, reactivos de fase aguda (CRP y ESR), factor reumatoide y anticuerpos antipéptido cíclico-citrulinado. La actividad de la enfermedad se midió mediante la puntuación de actividad de la enfermedad 28 (DAS28). Resultados: Los niveles de corte de RDW y MPV fueron 14,85 y 11,25. Los pacientes con RDW>14,85 tenían mayor puntuación de DAS28 (p=0,0003), ESR (p=0,0001) y CRP (p=0,0001). RDW se correlacionó positivamente con marcadores de actividad de la enfermedad (ESR, CRP y DAS28) en pacientes con AR. Sin embargo, DAS28 no era diferente entre los pacientes con MPV>11,25 y <11,25. Conclusión: RDW estaba fuertemente correlacionado con la actividad de la enfermedad. Además, RDW fue mejor que ESR y CRP en la detección de la actividad de la enfermedad de AR. De acuerdo con estos hallazgos, sugerimos que el RDW se puede utilizar en clínicas para seguir la actividad de la enfermedad. Además, RDW está ampliamente disponible, ya que por lo general se incluye en la imagen de sangre completa de rutina y no habrá necesidad de costos adicionales.(AU)
Subject(s)
Humans , Erythrocytes , Mean Platelet Volume , Arthritis, Rheumatoid/diagnosis , Arthritis, Rheumatoid/drug therapy , Correlation of Data , Cross-Sectional Studies , RheumatologyABSTRACT
Purpose: This study aimed to assess PIM-2 gene expression level as a prognostic marker in AML patients and to correlate the results with their clinical outcome. Patients and Methods: This study was conducted on 50 de novo younger AML patients (median age 44). Quantitative real-time polymerase chain reaction (QRT-PCR) was used to assess the expression level of the PIM-2 gene. The transcription level of the target gene (PIM-2) was normalized to that of the reference gene (GAPDH). Twenty control samples were withdrawn from 20 age- and sex-matched individuals for the analysis of the results using the 2-ΔΔCT method. On day 28 following induction chemotherapy, patients' bone marrow (BM) was examined for evaluation of their remission status. Results: PIM-2 gene expression was higher among AML patients who did not achieve complete remission (CR); also, it was higher in patients in the intermediate and poor cytogenetic risk groups. A significant positive correlation was found between PIM-2 level and BM blasts on day 28. In AML patients, PIM-2 has been discovered to be an independent predictive factor for achieving CR following standard induction treatment. Receiver operating characteristic curve (ROC) and area under the curve (AUC) were performed for PIM-2 level at diagnosis to evaluate its role in achieving remission after induction. It was found that PIM-2 at cutoff ≤1.6 had an AUC (0.903) with a sensitivity (90.48%) and specificity (86.21%), P <0.001. Conclusion: Overexpression of the PIM-2 gene is associated with induction failure and low CR.
ABSTRACT
INTRODUCTION: Hemogram parameters have been recently proposed as markers of inflammation in various studies from different parts of the world. Two of these hemogram parameters are red cell distribution width (RDW) and mean platelet volume (MPV). AIM: To evaluate the relation between RDW and MPV with disease activity of rheumatoid arthritis. To assess whether RDW and MPV can be used to follow disease activity in RA patients. METHODS: This is an observational cross-sectional study that was carried out on 60 rheumatoid arthritis patients who fulfilled the ACR/EULAR2010 classification criteria of RA attending to Rheumatology and Rehabilitation inpatient and outpatient clinics at Zagazig University Hospitals. All cases were subjected to full history taking, clinical examination, and laboratory investigations; differential complete blood picture (CBC), acute phase reactants (CRP and ESR), rheumatoid factor (RF) and anti-cyclic-citrullinated peptide (anti-CCP) antibodies. Disease activity was measured by disease activity score 28 (DAS28). RESULTS: The cut-off levels of RDW and MPV were 14.85 and 11.25. Patients with RDW>14.85 had higher Disease Activity Score 28 (DAS28; p=0.0003), ESR (p=0.0001) and CRP (p=0.0001). RDW was positively correlated with disease activity markers (ESR, CRP and DAS28) in rheumatoid arthritis patients. But, DAS28 was not different between patients with MPV>11.25 and <11.25. CONCLUSION: RDW was strongly correlated with disease activity. Also, RDW was better than ESR and CRP in detecting RA disease activity. According to these findings we suggest that RDW can be used in clinics to follow disease activity. In addition, RDW is widely available; as it's usually included in routine complete blood picture and there will be no need for further cost.
