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Oncotarget ; 7(50): 83319-83329, 2016 Dec 13.
Article in English | MEDLINE | ID: mdl-27825111

ABSTRACT

As a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to validate the previously reported 6-gene (LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2) model as predictor of prognosis in DLBCL. To avoid variation, all samples were tested in a single centre and single platform. This study comprised 8 countries (Brazil, Chile, Hungary, India, Philippines, S. Korea, Thailand and Turkey). Using the Kaplan-Meier and log rank test on patients (n=162) and two mortality risk groups (with those above and below the mean representing high and low risk groups) confirmed that the 6-gene predictor score correlates significantly with overall survival (OS, p<0.01) but not with event free survival (EFS, p=0.18). Adding the International Prognostic Index (IPI) shows that the 6-gene predictor score correlates significantly with high IPI scores for OS (p<0.05), whereas those with low IPI scores show a trend not reaching significance (p=0.08). This study defined an effective and economical qRT-PCR strategy and validated the 6-gene score as a predictor of OS in an international setting.


Subject(s)
Biomarkers, Tumor/genetics , Fixatives/chemistry , Formaldehyde/chemistry , Gene Expression Profiling/methods , Lymphoma, Large B-Cell, Diffuse/genetics , Paraffin Embedding , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , Tissue Fixation/methods , Transcriptome , Aged , Asia , Biopsy , Disease-Free Survival , Europe , Female , Gene Expression Profiling/standards , Genetic Predisposition to Disease , Humans , Kaplan-Meier Estimate , Lymphoma, Large B-Cell, Diffuse/drug therapy , Lymphoma, Large B-Cell, Diffuse/mortality , Lymphoma, Large B-Cell, Diffuse/pathology , Male , Middle Aged , Phenotype , Predictive Value of Tests , Proportional Hazards Models , Real-Time Polymerase Chain Reaction/standards , Reproducibility of Results , Reverse Transcriptase Polymerase Chain Reaction/standards , South America , Time Factors
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