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Vet Microbiol ; 144(3-4): 461-5, 2010 Aug 26.
Article in English | MEDLINE | ID: mdl-20197218

ABSTRACT

Severe outbreaks of diarrhoeic syndrome occurred in young foals at the same stud farm during two consecutive breeding periods namely spring 2006 and 2007. Rotavirus-like particles were detected by electron microscopy in the faeces of the affected foals and group A rotavirus infection was confirmed by Reverse-Transcription (RT)-PCR with selected sets of rotavirus-specific primers. Sequence analysis of the genes encoding the outer capsid rotavirus proteins VP7 and VP4 enabled classification of the viruses as G3AP[12] and revealed that the viruses were highly similar to recently reported equine rotavirus strains circulating in Europe. All Greek equine rotavirus isolates were genetically identical, suggesting persistence of the same viral strain in the stud farm, over the two consecutive foaling periods.


Subject(s)
Horse Diseases/virology , Rotavirus Infections/veterinary , Amino Acid Sequence , Animals , Antigens, Viral/chemistry , Antigens, Viral/genetics , Antigens, Viral/metabolism , Capsid Proteins/chemistry , Capsid Proteins/genetics , Capsid Proteins/metabolism , Disease Outbreaks/veterinary , Enzyme-Linked Immunosorbent Assay , Gene Expression Regulation, Viral , Greece/epidemiology , Horse Diseases/epidemiology , Horses , Microscopy, Electron, Transmission , Molecular Sequence Annotation , Molecular Sequence Data , Negative Staining , Rotavirus/classification , Rotavirus/genetics , Rotavirus Infections/epidemiology , Rotavirus Infections/virology
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