ABSTRACT
A novel member of the genus Amycolatopsis was isolated from soil collected from the banks of the Umgeni River, KwaZulu Natal province, South Africa. The strain, designated UM16(T), grouped with the type strains of Amycolatopsis alba, Amycolatopsis coloradensis and Amycolatopsis thailandensis by 16S rRNA gene based phylogeny. Genetic distance values, based on the gyrB and recN genes, between strain UM16(T) and its closest relatives were all above the threshold values of 0.02 and 0.04, respectively, that have been proposed to distinguish Amycolatopsis type strains. DNA-DNA hybridisation experiments confirmed that strain UM16(T) represents a unique genomic species, sharing 18.4 ± 5.1, 16.2 ± 1.8 and 45.8 ± 8.9 % DNA relatedness to the type strains of A. alba, A. coloradensis and A. thailandensis, respectively. The physiological, phylogenetic and DNA-relatedness data support the description of strain UM16(T) as the type strain of a novel species, for which the name Amycolatopsis umgeniensis sp. nov. is proposed (= DSM 45272(T) = NRRL B-24724(T)).
Subject(s)
Actinomycetales/classification , Actinomycetales/isolation & purification , Soil Microbiology , Actinomycetales/genetics , Actinomycetales/physiology , Bacterial Proteins/genetics , Bacterial Typing Techniques , Cluster Analysis , DNA Gyrase/genetics , DNA Restriction Enzymes/genetics , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Molecular Sequence Data , Nucleic Acid Hybridization , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , South AfricaABSTRACT
An actinomycete with blue substrate mycelium was isolated from a soil sample in Cape Town, South Africa, and designated strain PK-Blue(T). The colour of the substrate mycelium was not sensitive to changes in pH. The organism produced hairy spores in Spirales-type spore chains. Chemical taxonomy indicated that it belonged to the genus Streptomyces. Strain PK-Blue(T) produced no diffusible pigments other than melanin, grew at 45 degrees C, did not degrade adenine and exhibited no antibacterial activity against Enterococcus faecium, Escherichia coli or Pseudomonas aeruginosa. Analysis of its 16S rRNA gene sequence and the results of physiological tests showed that strain PK-Blue(T) (= DSM 41797(T) = ATCC BAA-411(T)) represents the type strain of a novel species of Streptomyces, for which the name Streptomyces speibonae sp. nov. is proposed.
