Antioxidant effect of MCI-186, a new Free-Radical scavenger, on ischemia-reperfusion injury in a rat hindlimb amputation model.

BACKGROUND: A newly synthesized free-radical scavenger, MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-1), was recently approved in Japan for treating acute brain infarction. The purpose of this study was to investigate whether or not MCI-186 is effective in reducing ischemia-reperfusion injury in the extremities. MATERIALS AND METHODS: Warm ischemia was sustained for 4 hours. The animals were divided into 4 groups: (1) sham group, (2) control group (saline injection), (3) MCI group (MCI-186 injection), and (4) EPC group (EPC-K1 [(l-ascorbic acid 2-[3,4-dihydro-2, 5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl hydrogen phosphate] potassium salt], a hydroxyl-radical scavenger, injection). Wet and dry (W/D) weights of the gastrocnemius and tibialis anterior muscles, muscle contractile function, and serum levels of creatine phosphokinase (CPK), lactate dehydrogenase (LDH), glutamic oxaloacetic transminase (GOT), and mitochondrial glutamic oxaloacetic transminase (GOT-m) were measured after 24 h of reperfusion. The cytotoxic aldehydes malondialdehyde and 4-hydroxy-2-nonenal as indices of lipid peroxidation (LPO), and neutrophil-specific enzyme myeloperoxidase (MPO) as an index of neutrophil infiltration, were measured in the gastrocnemius muscle. RESULTS: Contractile functions in the MCI and EPC groups were significantly better than in the control group. In the tibialis anterior muscle, the contractile function was better in the MCI group than in the EPC group. W/D ratios and serum levels of CPK, LDH, GOT, and GOT-m were lower in the sham and MCI groups than in the control group. Levels of LPO and MPO activity were significantly lower in the MCI and EPC groups than in the control group. Histological findings demonstrated that inflammatory tissue reactions rarely occurred in the MCI group. CONCLUSION: MCI-186 is effective in preventing ischemia-reperfusion injury in extremities. MCI-186 seems to have promise as a therapeutic agent, because it prevents ischemia-reperfusion injury even in the tibialis anterior muscle, which contains fast-twitch glycolytic fibers, known to be very susceptible to ischemic insult.

Birds of a central São Paulo woodlot: 1. Censuses 1982-2000.

Some 263 birds were recorded near and in a 230-ha patch of semideciduous forest in cane fields of central São Paulo, Brazil. Subtracting 67 open-area species, 22 of marshes or creeks, 10 vagrants and 12 recorded later, 152 forest and border species were recorded in 1982-86, much like what was observed in a similar woodlot near Campinas. Both woodlots lost species gradually over the years. Some birds avoided hard cane-field edges, preferring soft bushy edges. Of open-area species, 22 seemed to have disappeared by 1997 due to earlier high El Niño rains or rare permanently open habitats in the sugar cane; 17 new species were mostly nocturnal ones not noted earlier, or occasional visitors. Forest and borders lost 31 species, gaining five of dry regions and one winter visitor. Several migrants from the south appeared only in wet years before recent greenhouse effects, some resident birds were hunted, and canopy hummingbirds were perhaps still present. Dry-forest travel-prone or "metapopulational" species moved their centers of distribution, partly concealing loss of moist-forest diversity.

Birds of a central São Paulo woodlot: 1. Censuses 1982-2000

Some 263 birds were recorded near and in a 230-ha patch of semideciduous forest in cane fields of central São Paulo, Brazil. Subtracting 67 open-area species, 22 of marshes or creeks, 10 vagrants and 12 recorded later, 152 forest and border species were recorded in 1982-86, much like what was observed in a similar woodlot near Campinas. Both woodlots lost species gradually over the years. Some birds avoided hard cane-field edges, preferring soft bushy edges. Of open-area species, 22 seemed to have disappeared by 1997 due to earlier high El Niño rains or rare permanently open habitats in the sugar cane; 17 new species were mostly nocturnal ones not noted earlier, or occasional visitors. Forest and borders lost 31 species, gaining five of dry regions and one winter visitor. Several migrants from the south appeared only in wet years before recent greenhouse effects, some resident birds were hunted, and canopy hummingbirds were perhaps still present. Dry-forest travel-prone or "metapopulational" species moved their centers of distribution, partly concealing loss of moist-forest diversity.

Foram listadas 263 aves próximo e dentro de uma mata semidecídua de 230 ha em meio a plantações de cana-de-açúcar, na região central do Estado de São Paulo, Brasil. Subtraindo-se 67 espécies de áreas abertas, 22 de riachos, 10 vagantes e 12 observadas mais tarde, cerca de 152 espécies de mata e de borda foram observadas entre 1982-86, semelhantemente às observadas em uma mata próxima a Campinas. Ambas as matas perderam espécies gradualmente. Algumas aves evitaram as margens "duras" dos canaviais, preferindo as margens "macias" arbustivas. Das espécies de área aberta, parece que 22 desapareceram até 1997, em razão das abundantes chuvas anteriores causadas por El Niño, ou pela raridade de habitats permanentemente abertos nos canaviais; 17 espécies "novas" eram principalmente noturnas que não haviam sido checadas anteriormente, ou visitantes ocasionais. A mata e as bordas perderam 37 espécies, ganhando 5 de regiões secas e 1 visitante de inverno. Vários migrantes do sul apareceram somente em anos chuvosos antes do recente efeito estufa; algumas aves residentes foram caçadas, e os beija-flores das copas talvez estivessem presentes ainda. As espécies de zonas secas, prones à movimentação ou "metapopulacionais", movem os centros de distribuição, camuflando parcialmente a perda de diversidade da mata úmida.

Birds of a central Säo Paulo woodlot: 1. Censuses 1982-2000

Some 263 birds were recorded near and in a 230-ha patch of semideciduous forest in cane fields of central Säo Paulo, Brazil. Subtracting 67 open-area species, 22 of marshes or creeks, 10 vagrants and 12 recorded later, 152 forest and border species were recorded in 1982-86, much like what was observed in a similar woodlot near Campinas. Both woodlots lost species gradually over the years. Some birds avoided hard cane-field edges, preferring soft bushy edges. Of open-area species, 22 seemed to have disappeared by 1997 due to earlier high El Ni¤o rains or rare permanently open habitats in the sugar cane; 17 new species were mostly nocturnal ones not noted earlier, or occasional visitors. Forest and borders lost 31 species, gaining five of dry regions and one winter visitor. Several migrants from the south appeared only in wet years before recent greenhouse effects, some resident birds were hunted, and canopy hummingbirds were perhaps still present. Dry-forest travel-prone or "metapopulational" species moved their centers of distribution, partly concealing loss of moist-forest diversity

Effect of perfusion during ischemia on skeletal muscle.

BACKGROUND: Despite the established preservation method for major organs of perfusion followed by immersion at hypothermia, a standard preservation technique for skeletal muscle is still a matter of controversy. The purpose of this study is to examine the effect of perfusion on the preservation of skeletal muscle in amputated limbs. MATERIALS AND METHOD: The rat hindlimb was amputated for perfusion with Euro-Collins (EC) or University of Wisconsin (UW) solution at different perfusion pressures (40 or 100 cm-gravity). After certain ischemic periods (4 or 5 h), the skeletal muscle viability was determined by measuring the tissue content of adenosine triphosphate (ATP). RESULT: The UW solution perfusion group maintained better ATP levels than the EC solution group when the ischemic period was extended to 5 h. The perfusion pressure of 100 cm-gravity was more effective for preserving muscle viability than 40 cm-gravity with both EC and UW solutions. CONCLUSION: UW solution might be adequate to preserve muscle viability and perfusion pressure is recommended at 100 cm-gravity rather than at minimal pressure (40 cm-gravity), which washes out stagnant blood.

Nesting behavior of the swallow-tailed hummingbird, Eupetomena macroura (Trochilidae, aves)

An August or winter nestling of Eupetomena macroura was fed only every 40-50 min for at least 24 days in the nest, with fewer feedings at midday. As in other hummingbirds, it was brooded only the first week or two, and left alone even on cool nights after 12 days, probably due to the small nest size. The female attacked birds of many non-nectarivore species near the nest, in part probably to avoid predation. Botfly parasitism was extremely high, as in some other forest-edge birds

Nesting behavior of the Picazuro Pigeon, Columba Picazuro (Columbidae, aves)

The Picazuro Pigeon nests in all months of the year in southeastern Brazil. Nest material is plucked from trees or ground and carried to build a frail and transparent nest of sticks where one egg is laid. Female and male alternate in incubation and brooding and do not soil the nest with feces

Nesting behavior of the swallow-tailed hummingbird, Eupetomena macroura (Trochilidae, Aves).

An August or winter nestling of Eupetomena macroura was fed only every 40-50 min for at least 24 days in the nest, with fewer feedings at midday. As in other hummingbirds, it was brooded only the first week or two, and left alone even on cool nights after 12 days, probably due to the small nest size. The female attacked birds of many non-nectarivore species near the nest, in part probably to avoid predation. Botfly parasitism was extremely high, as in some other forest-edge birds.

Nesting behavior of the Picazuro pigeon, Columba Picazuro (Columbidae, Aves).

The Picazuro Pigeon nests in all months of the year in southeastern Brazil. Nest material is plucked from trees or ground and carried to build a frail and transparent nest of sticks where one egg is laid. Female and male alternate in incubation and brooding and do not soil the nest with feces.

Effect of contamination and etching on enamel bond strength of new light-cured glass ionomer cements.

The effect of water and saliva contamination on the bond strength of metal orthodontic brackets cemented to etched (10% polyacrylic acid) and unetched human premolar enamel was investigated. Two bonding agents were used: one commercially available product (LC) and one experimental (EX) light-cured glass ionomer. Shear bond strength was measured after aging for 5 minutes, 15 minutes, and 24 hours. The results were compared by ANOVA and Scheffe's tests at p = 0.05. For LC, the bond strength of brackets bonded to etched enamel, with and without contamination, was statistically higher than that of brackets bonded to unetched enamel for all aging times. An exception was the bond strength to unetched enamel with saliva contamination after 24 hours; for EX, this value was statistically higher than that measured on unetched enamel with water contamination. Contamination by saliva did not reduce bond strength to unetched enamel. For both etched and unetched enamel, there was no significant difference between LC and EX after 24 hours for all contamination conditions.

Opportunistic adaptations to man-induced habitat changes by some South American caprimulgidae.

We describe the opportunistic adaptation to man-induced changes in the habitats of six neotropical Caprimulgidae. Habitats created by those changes, and similar to their original and usual ones, are readily occupied by these nightjars. The occasional invasion of urban environments ("urbanization") is the most recent and most remarkable behavioural adaptation.