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ABSTRACT

We investigated the effects of treatment with anti-parathyroid hormone-related protein (1-34) monoclonal murine antibody (anti-PTHrP MoAb) on apoptosis and the differentiation of chondrosarcoma HTB-94 cells. Treatment with anti-PTHrP MoAb accelerated apoptosis of HTB-94 cells in a dose-dependent manner, and anti-PTHrP MoAb also promoted the chondrogenic differentiation of HTB-94 cells. The induction of apoptosis by anti-PTHrP MoAb via imbalance of Bcl-2/Bax ratio and activation of caspase-3 may provide a mechanistic explanation for its potential antitumor effects. Our results suggest the possibility that anti-PTHrP MoAb may be beneficial as a new treatment for chondrosarcoma.

Subject(s)

Antibodies, Monoclonal/pharmacology , Apoptosis/drug effects , Bone Neoplasms/pathology , Cell Differentiation/drug effects , Chondrosarcoma/pathology , Parathyroid Hormone-Related Protein/immunology , Bone Neoplasms/immunology , Bone Neoplasms/metabolism , Caspase 3 , Caspases/metabolism , Cell Division/drug effects , Chondrosarcoma/immunology , Chondrosarcoma/metabolism , Collagen Type II/genetics , Collagen Type II/metabolism , Collagen Type X/genetics , Collagen Type X/metabolism , DNA Primers/chemistry , Humans , Immunoenzyme Techniques , Peptide Fragments/immunology , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured , bcl-2-Associated X Protein

ABSTRACT

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