ABSTRACT
The antimicrobial effect of cold water, hot water and ethanolic extracts of Spondias mombin and Baphia nittida on cariogenic streptococci isolated from dental caries patients attending the Ebonyi State University Teaching hospital dental clinic Abakaliki was investigated using the agar well diffusion technique. The cold water and ethanolic extracts of Baphia nittida showed inhibition zone diameter (IZD) of 10 and 12 mm respectively at 400 mg mL(-1), while the hot water showed no inhibitory effect. All extracts of Spondias mombin did not inhibit the test organism. The cold water and ethanolic extracts of Baphia nittida showed Minimum Inhibitory Concentration (MIC) of 100 and 50 mg mL(-1) respectively. The combination of the cold water extracts of the two herbs showed enhanced activity of 13 mm. Phytochemical analysis of Baphia nittida revealed the presence of flavonoids, glycosides, proteins saponins, tannins, carbohydrate and steroidal aglycone. Acute toxicity testing of Baphia nittida at a range of 250-5000 mg kg(-1) bw using mice showed no clinical signs of acute toxicity. No chemical toxicity was observed amongst rats given Baphia nittida extracts 500 and 1000 mg kg(-1) bw after 30 days. Baphia nittida may be a potential source of an antimicrobial agent for the treatment and management of dental caries.
Subject(s)
Anacardiaceae/chemistry , Anti-Bacterial Agents/pharmacology , Dental Caries/microbiology , Dental Caries/therapy , Fabaceae/chemistry , Animals , Body Weight/drug effects , Male , Mice , Plant Extracts/chemistry , Plant Extracts/pharmacology , Rats , Streptococcus/drug effectsABSTRACT
Fungi invasion of our agricultural products and food lead to mouldiness and production of mycotoxin . These mycotoxin are toxic secondary metabolites elaborated by the fungi. They can cause diseases generally referred to as mycotoxicoses; the fungi per se are not involved in the disease process. One of such secondary metabolites of mouldiness is aflatoxin (which are exotoxin). The aflatoxin - producing species of fungi belong to the genus aspergillus Micheli 1729; which forms a large proportion of all the moulds encountered in our agricultural and industrial works. Their contamination of the foods and feeds (maize; sorghum; millet; rice; wheat soyabean etc) constitute an important toxicological hazard
