ABSTRACT
This is a comparative study on the adequacy of cervical smears obtained using the Papcone® sampling device or wooden Ayre's spatula conducted from two tertiary health facilities -- Nnamdi Azikiwe University Teaching Hospital Nnewi and Chukwuemeka Odumegwu Ojukwu University Teaching Hospital Awka, in Anambra State, Nigeria. Slides from smears obtained using both devices were read by a cytopathologist blinded for the study. The primary outcome was the proportion of smears with an adequate endocervical component. Significantly higher adequate cervical smears were obtained in 177/192 (92.2%) women using the Papcone® sampling device, compared to 152/192 (79.2%) using wooden Ayre's spatula (p < 0.001). Kappa analysis showed moderate inter-rater agreement between the two devices. We recommend the use of the Papcone device when it is available, as the adequacy of cervical smears obtained with the Papcone® was better than that obtained using wooden Ayre's spatula.
ABSTRACT
BACKGROUND: Chemical contaminants have been found to affect reproductive functions in mammals. This study investigated the effect of Chevron Escravos crude oil and Emulsol L.W. dispersant on the testicular functions of guinea pig. METHODS: Eight groups of seven sexually mature male guinea pigs each were given 1250, 2500, or 5000 mg/kg of crude oil and dispersant for 7 days. The fluid and food intake and body weight of the animals were measured daily throughout the study. After the exposure period, sperm quality analysis was carried out, and fructose and lactate dehydrogenase were analyzed in tissue homogenate, while testosterone and estradiol were assayed in blood. The right testis was also processed for histological analysis. RESULTS: The epididymal sperm number and fructose level of treated animals showed a significant dose-dependent decrease (p<0.05) compared with controls. Also, sperm motility and morphology were altered in the treated groups, while testosterone and estradiol levels were increased significantly (p<0.05) in the treated groups. Histological examination showed signs of toxicity in the treated animals. CONCLUSIONS: From the findings, it was evident that Chevron Escravos crude oil and Emulsol L.W. oil dispersant are able to cause acute testicular toxicity in guinea pigs. The possible mechanism of toxicity is suggested to be by stimulation of hormone production from the adrenal cortex, causing a negative feedback on gonadotropin-releasing hormone in the pituitary gland to suppress spermatogenesis.
