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1.
Solid State Nucl Magn Reson ; 93: 1-6, 2018 09.
Article in English | MEDLINE | ID: mdl-29758460

ABSTRACT

Double and zero quantum filtered (ZQF/DQF) 2H NMR spectroscopy was used to study D2O in five different Nafion membranes, N117, N115, NR212, XL, and HP, in order to assess the effectiveness of the technique for monitoring differences in thickness, membrane reinforcement, and the addition of chemical stabilizers. Experiments were also carried out at 20 and 80 °C to understand if the ZQF/DQF technique could detect changes in the water environments and exchange dynamics as a function of temperature. For two of the membranes, significant decreases in the 1/T2 relaxation rates were observed at 80 °C. The two modified membranes showed changes in the quadrupolar couplings when heated, with the XL membranes showing a drop in the coupling and the HP membranes showing an increase in the coupling. No consistent variations could be associated with thickness, reinforcement or the addition of stabilizers. Overall the technique was able to detect some differences between the membranes but was limited by the variability in the observed NMR data.

2.
J Magn Reson ; 258: 6-11, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26150377

ABSTRACT

The analysis of double and zero quantum filtered (2)H NMR spectra obtained from D2O perfused in the nucleus pulposus of human intervertebral disc tissue samples is reported. Fitting the spectra with a three-site model allows for residual quadrupolar couplings and T2 relaxation times to be measured. The analysis reveals changes in both the couplings and relaxation times as the tissue begins to show signs of degradation. The full analysis demonstrates that information about tissue hydration, water collagen interactions, and sample heterogeneity can be obtained and used to better understand the biochemical differences between healthy and degraded tissue.


Subject(s)
Algorithms , Body Water/chemistry , Deuterium Oxide/analysis , Intervertebral Disc Displacement/metabolism , Intervertebral Disc/chemistry , Signal Processing, Computer-Assisted , Adolescent , Adult , Aged , Humans , Hydrogen/analysis , Intervertebral Disc Displacement/diagnosis , Male , Middle Aged , Reproducibility of Results , Sensitivity and Specificity , Young Adult
3.
J Phys Chem B ; 118(13): 3491-7, 2014 Apr 03.
Article in English | MEDLINE | ID: mdl-24628019

ABSTRACT

In an effort to provide insight into the molecular origins of the (2)H double quantum filtered (DQF) NMR signal observed in connective tissue, specifically spinal disc tissue, (2)H multiple quantum filtered (MQF) NMR spectroscopy is used to study the structure and dynamics of D2O in collagen as a function of hydration. Residual quadrupolar coupling constants are measured and decrease from 3500 to 20 Hz while T2 relaxation times increase from 0.65 to 20 ms as hydration increases. Analysis of the data indicates that the quadrupolar coupling and T2 relaxation arises when water molecules spend time in restricted environments. The residual quadrupolar coupling is influenced almost exclusively by the most restricted water sites, the clefts of the triple helices not exposed on the surface of the fibrils, while the T2 relaxation has secondary contributions from less restricted water environments. The magnitudes of the measured values are consistent with results from DQF NMR studies of spinal disc tissue, supporting the assertion that water binding to collagen is a major contributor to the DQF NMR signal observed in spinal disc tissue.


Subject(s)
Collagen Type I/chemistry , Water/chemistry , Animals , Cattle , Deuterium Oxide/chemistry , Magnetic Resonance Spectroscopy , Quantum Theory
4.
J Phys Chem B ; 116(50): 14724-30, 2012 Dec 20.
Article in English | MEDLINE | ID: mdl-23214923

ABSTRACT

Double and zero quantum filtered (2)H NMR spectroscopy is used to study the structure and dynamics of D(2)O in sulfonated poly(ether ether ketone) membranes as a function of membrane hydration. Both residual quadrupolar coupling constants and T(2) relaxation values are obtained as a function of hydration. The residual couplings vary from 160 Hz at low hydration to 30 Hz at high hydration. The T(2) relaxation times range from 3 to 14 ms, with the high hydration values having longer T(2). Results from this study are compared to results obtained for Nafion membranes, revealing similarities and differences in the water environments of the two membranes that result from the structure of the polymers and can be related to properties such as water diffusion.

5.
Phys Chem Chem Phys ; 11(15): 2690-9, 2009 Apr 21.
Article in English | MEDLINE | ID: mdl-19421527

ABSTRACT

Five octahedral Co(iii) cations, [trans-Co(en)(2)(X)((13)CH(3))](n+) where en = ethylenediamine, X = CN(-), N(3)(-), NH(3), NO(2)(-) or H(2)O and n = 1 or 2, as well as [Co(NH(3))(5)(13)CH(3)](2+), have been investigated by solid-state (13)C and (59)Co NMR spectroscopy. We show that the determination of the (59)Co nuclear quadrupolar parameters both directly via(59)Co NMR and indirectly via(13)C NMR provide complementary information that is unavailable if one investigates only one nucleus. Specifically, (1)J((59)Co,(13)C) and the orientation of the largest component of the EFG were determined via(13)C NMR spectroscopy, which also established the negative sign of C(Q)((59)Co). Cobalt-59 NMR spectroscopy was used to characterize the cobalt magnetic shielding tensor, to verify the magnitudes of C(Q)((59)Co) and to establish the value of eta(Q), which is difficult to determine indirectly. The measurements show that the EFG tensors are either axially symmetric or close to being so, but there is a wide range of C(Q) values, from -40 MHz for the complex with X = H(2)O to -105 MHz with X = CN(-). The Co chemical shift tensors are approximately axially symmetric with the spans, delta(11)-delta(33), ranging from 3700 to 5600 ppm for X = H(2)O and CN(-), respectively. The latter measurements also established the relative orientations of the Co EFG and chemical shift tensors. Density functional theory calculations of the (59)Co EFG and magnetic shielding tensors as well as of (1)J((59)Co,(13)C) for the NO(2)(-) and N(3)(-) complexes were undertaken. These calculations confirm the experimental observation that the sign of C(Q) is negative and that the largest component of the EFG is along the Co-methyl-carbon bond.

6.
Dalton Trans ; (17): 3262-9, 2009 May 07.
Article in English | MEDLINE | ID: mdl-19421628

ABSTRACT

Using (51)V magic angle spinning solid-state NMR spectroscopy and density functional theory calculations we have characterized the chemical shift and quadrupolar coupling parameters for two eight-coordinate vanadium complexes, [PPh(4)][V(v)(HIDPA)(2)] and [PPh(4)][V(v)(HIDA)(2)]; HIDPA = 2,2'-(hydroxyimino)dipropionate and HIDA = 2,2'-(hydroxyimino)diacetate. The coordination geometry under examination is the less common non-oxo eight coordinate distorted dodecahedral geometry that has not been previously investigated by solid-state NMR spectroscopy. Both complexes were isolated by oxidizing their reduced forms: [V(iv)(HIDPA)(2)](2-) and [V(iv)(HIDA)(2)](2-). V(iv)(HIDPA)(2)(2-) is also known as amavadin, a vanadium-containing natural product present in the Amanita muscaria mushroom and is responsible for vanadium accumulation in nature. The quadrupolar coupling constants, C(Q), are found to be moderate, 5.0-6.4 MHz while the chemical shift anisotropies are relatively small for vanadium complexes, -420 and -360 ppm. The isotropic chemical shifts in the solid state are -220 and -228 ppm for the two compounds, and near the chemical shifts observed in solution. Presumably this is a consequence of the combined effects of the increased coordination number and the absence of oxo groups. Density functional theory calculations of the electric field gradient parameters are in good agreement with the NMR results while the chemical shift parameters show some deviation from the experimental values. Future work on this unusual coordination geometry and a combined analysis by solid-state NMR and density functional theory should provide a better understanding of the correlations between experimental NMR parameters and the local structure of the vanadium centers.


Subject(s)
Alanine/analogs & derivatives , Hydroxamic Acids/chemistry , Magnetic Resonance Spectroscopy/methods , Vanadium/chemistry , Agaricales/chemistry , Alanine/chemistry , Models, Molecular , Models, Theoretical , Molecular Conformation , Oxidation-Reduction
7.
J Magn Reson ; 195(1): 112-5, 2008 Nov.
Article in English | MEDLINE | ID: mdl-18774321

ABSTRACT

A method for acquiring triple quantum filtered (TQF) (23)Na NMR images is proposed that takes advantage of the differences in transverse relaxation rates of sodium to achieve positive intensity, PI, NMR signal. This PITQF imaging sequence has been used to obtain spatially resolved one-dimensional images as a function of the TQF creation time, tau, for two human spinal disc samples. From the images the different parts of the tissue, nucleus pulposus and annulus fibrosus, can be clearly distinguished based on their signal intensity and creation time profiles. These results establish the feasibility of (23)Na TQF imaging and demonstrate that this method should be applicable for studying human disc tissues as well as spinal disc degeneration.


Subject(s)
Intervertebral Disc Displacement/metabolism , Intervertebral Disc/chemistry , Magnetic Resonance Imaging/methods , Magnetic Resonance Spectroscopy/methods , Sodium Isotopes/chemistry , Aged , Female , Humans , Male , Staining and Labeling/methods
8.
Magn Reson Med ; 60(2): 246-52, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18666105

ABSTRACT

Degenerative disc disease is an irreversible process that leads to a loss of mechanical integrity and back pain in millions of people. In this report, (23)Na double-quantum-filtered (DQF) NMR spectroscopy is used to study disc tissues in two stages of degeneration. Initial results indicate that the (23)Na DQF signal may be useful for determining the degree of degeneration. The spectral analysis reveals the presence of sodium environments with different residual quadrupolar couplings and T(2) relaxation times that we attribute to different regions, or compartments, corresponding to different biochemical regions in the tissue. In general it is found that there are compartments with no residual quadrupolar couplings, compartments with moderate couplings (200 to 1000 Hz), and compartments with couplings ranging from 1500 to 3000 Hz. The results indicate that (23)Na DQF NMR spectroscopy provides a probe of the degenerative state of the intervertebral disc tissues, and might hold potential as a novel diagnostic method for detection of disc degeneration.


Subject(s)
Body Water/chemistry , Diagnosis, Computer-Assisted/methods , Intervertebral Disc Displacement/diagnosis , Intervertebral Disc Displacement/metabolism , Magnetic Resonance Spectroscopy/methods , Water/analysis , Adolescent , Aged , Humans , Signal Processing, Computer-Assisted , Sodium Isotopes/analysis , Sodium Isotopes/chemistry
9.
J Chem Phys ; 128(5): 052317, 2008 Feb 07.
Article in English | MEDLINE | ID: mdl-18266434

ABSTRACT

(51)V solid-state NMR and density functional theory (DFT) investigations are reported for a series of pentacoordinate dioxovanadium(V)-dipicolinate [V(V)O(2)-dipicolinate] and heptacoordinate aquahydroxylamidooxovanadium(V)-dipicolinate [V(V)O-dipicolinate] complexes. These compounds are of interest because of their potency as phosphatase inhibitors as well as their insulin enhancing properties and potential for the treatment of diabetes. Experimental solid-state NMR results show that the electric field gradient tensors in the V(V)O(2)-dipicolinate derivatives are affected significantly by substitution on the dipicolinate ring and range from 5.8 to 8.3 MHz. The chemical shift anisotropies show less dramatic variations with respect to the ligand changes and range between -550 and -600 ppm. To gain insights on the origins of the NMR parameters, DFT calculations were conducted for an extensive series of the V(V)O(2)- and V(V)O-dipicolinate complexes. To assess the level of theory required for the accurate calculation of the (51)V NMR parameters, different functionals, basis sets, and structural models were explored in the DFT study. It is shown that the original x-ray crystallographic geometries, including all counterions and solvation water molecules within 5 A of the vanadium, lead to the most accurate results. The choice of the functional and the basis set at a high level of theory has a relatively minor impact on the outcome of the chemical shift anisotropy calculations; however, the use of large basis sets is necessary for accurate calculations of the quadrupole coupling constants for several compounds of the V(V)O(2) series. These studies demonstrate that even though the vanadium compounds under investigations exhibit distorted trigonal bipyramidal coordination geometry, they have a "perfect" trigonal bipyramidal electronic environment. This observation could potentially explain why vanadate and vanadium(V) adducts are often recognized as potent transition state analogs.


Subject(s)
Organometallic Compounds/chemistry , Picolinic Acids/chemistry , Vanadium , Isotopes , Magnetic Resonance Spectroscopy/methods
10.
Inorg Chem ; 46(22): 9285-93, 2007 Oct 29.
Article in English | MEDLINE | ID: mdl-17902653

ABSTRACT

Using (51)V magic angle spinning solid-state NMR, SSNMR, spectroscopy and quantum chemical DFT calculations we have characterized the chemical shift and quadrupolar coupling parameters of a series of eight hydroxylamido vanadium(V) dipicolinate complexes of the general formula VO(dipic)(ONR1R2)(H2O) where R1 and R2 can be H, CH3, or CH2CH3. This class of vanadium compounds was chosen for investigation because of their seven-coordinate vanadium atom, a geometry for which there is limited (51)V SSNMR data. Furthermore, a systematic series of compounds with different electronic properties are available and allows for the effects of ligand substitution on the NMR parameters to be studied. The quadrupolar coupling constants, C(Q), are small, 3.0-3.9 MHz, but exhibit variations as a function of the ligand substitution. The chemical shift tensors in the solid state are sensitive to changes in both the hydroxylamide substituent and the dipic ligand, a sensitivity which is not observed for isotropic chemical shifts in solution. The chemical shift tensors span approximately 1000 ppm and are nearly axially symmetric. On the basis of DFT calculations of the chemical shift tensors, one of the largest contributors to the magnetic shielding anisotropy is an occupied molecular orbital with significant vanadium d(z)2 character along the V=O bond.


Subject(s)
Magnetic Resonance Spectroscopy/methods , Picolinic Acids/chemistry , Vanadium/chemistry , Amides/chemistry , Quantum Theory
12.
Phys Chem Chem Phys ; 9(10): 1226-38, 2007 Mar 14.
Article in English | MEDLINE | ID: mdl-17325769

ABSTRACT

Central transition (55)Mn NMR spectra of several solid manganese pentacarbonyls acquired at magnetic field strengths of 11.75, 17.63, and 21.1 T are presented. The variety of distinct powder sample lineshapes obtained demonstrates the sensitivity of solid-state (55)Mn NMR to the local bonding environment, including the presence of crystallographically unique Mn sites, and facilitates the extraction of the Mn chemical shift anisotropies, CSAs, and the nuclear quadrupolar parameters. The compounds investigated include molecules with approximate C(4v) symmetry, LMn(CO)(5)(L = Cl, Br, I, HgMn(CO)(5), CH(3)) and several molecules of lower symmetry (L = PhCH(2), Ph(3-n)Cl(n)Sn (n= 1, 2, 3)). For these compounds, the Mn CSA values range from <100 ppm for Cl(3)SnMn(CO)(5) to 1260 ppm for ClMn(CO)(5). At 21.1 T the (55)Mn NMR lineshapes are appreciably influenced by the Mn CSA despite the presence of significant (55)Mn quadrupolar coupling constants that range from 8.0 MHz for Cl(3)SnMn(CO)(5) to 35.0 MHz for CH(3)Mn(CO)(5). The breadth of the solid-state (55)Mn NMR spectra of the pentacarbonyl halides is dominated by the CSA at all three applied magnetic fields. DFT calculations of the Mn magnetic shielding tensors reproduce the experimental trends and the magnitude of the CSA is qualitatively rationalized using a molecular orbital, MO, interpretation based on Ramsey's theory of magnetic shielding. In addition to the energy differences between symmetry-appropriate occupied and virtual MOs, the d-character of the Mn MOs is important for determining the paramagnetic shielding contribution to the principal components of the magnetic shielding tensor.

13.
Inorg Chem ; 45(21): 8492-9, 2006 Oct 16.
Article in English | MEDLINE | ID: mdl-17029359

ABSTRACT

55Mn NMR spectra acquired at 21.14 T (nu(L)(55Mn) = 223.1 MHz) are presented and demonstrate the advantages of using ultrahigh magnetic fields for characterizing the chemical shift tensors of several manganese carbonyls: eta5-CpMn(CO)3, Mn2(CO)10, and (CO)5MnMPh3 (M = Ge, Sn, Pb). For the compounds investigated, the anisotropies of the manganese chemical shift tensors are less than 250 ppm except for eta5-CpMn(CO)3, which has an anisotropy of 920 ppm. At 21.14 T, one can excite the entire m(I) = 1/2 <--> m(I) = -1/2 central transition of eta5-CpMn(CO)3, which has a breadth of approximately 700 kHz. The breadth arises from second-order quadrupolar broadening due to the 55Mn quadrupolar coupling constant of 64.3 MHz, as well as the anisotropic shielding. Subtle variations in the electric field gradient tensors at the manganese are observed for crystallographically unique sites in two of the solid pentacarbonyls, resulting in measurably different C(Q) values. MQMAS experiments are able to distinguish four magnetically unique Mn sites in (CO)(5)MnPbPh3, each with slightly different values of delta(iso), C(Q), and eta(Q).

14.
Chemistry ; 12(1): 159-68, 2005 Dec 16.
Article in English | MEDLINE | ID: mdl-16224769

ABSTRACT

Lanthanum-139 NMR spectra of stationary samples of several solid La(III) coordination compounds have been obtained at applied magnetic fields of 11.75 and 17.60 T. The breadth and shape of the 139La NMR spectra of the central transition are dominated by the interaction between the 139La nuclear quadrupole moment and the electric field gradient (EFG) at that nucleus; however, the influence of chemical-shift anisotropy on the NMR spectra is non-negligible for the majority of the compounds investigated. Analysis of the experimental NMR spectra reveals that the 139La quadrupolar coupling constants (C(Q)) range from 10.0 to 35.6 MHz, the spans of the chemical-shift tensor (Omega) range from 50 to 260 ppm, and the isotropic chemical shifts (delta(iso)) range from -80 to 178 ppm. In general, there is a correlation between the magnitudes of C(Q) and Omega, and delta(iso) is shown to depend on the La coordination number. Magnetic-shielding tensors, calculated by using relativistic zeroth-order regular approximation density functional theory (ZORA-DFT) and incorporating scalar only or scalar plus spin-orbit relativistic effects, qualitatively reproduce the experimental chemical-shift tensors. In general, the inclusion of spin-orbit coupling yields results that are in better agreement with those from the experiment. The magnetic-shielding calculations and experimentally determined Euler angles can be used to predict the orientation of the chemical-shift and EFG tensors in the molecular frame. This study demonstrates that solid-state 139La NMR spectroscopy is a useful characterization method and can provide insight into the molecular structure of lanthanum coordination compounds.

15.
Solid State Nucl Magn Reson ; 28(2-4): 125-34, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16125375

ABSTRACT

Results from a solid-state 139La NMR spectroscopic investigation of the anhydrous lanthanum(III) halides (LaX3; X=F, Cl, Br, I) at applied magnetic fields of 7.0, 9.4, 11.7, 14.1, and 17.6 T are presented and highlight the advantages of working at high applied magnetic field strengths. The 139La quadrupolar coupling constants are found to range from 15.55 to 24.0 MHz for LaCl3 and LaI3, respectively. The lanthanum isotropic chemical shifts exhibit an inverse halogen dependence with values ranging from -135 ppm for LaF3 to 700 ppm for LaI3, which represents nearly half of the total lanthanum chemical shift range. The spans of the magnetic shielding tensors also vary widely, from 35 to 650 ppm for the solid LaF3 through LaI3. DFT calculations of the 139La electric field gradient and magnetic shielding tensors have been performed and provide a qualitative interpretation of the trends observed experimentally.

16.
Org Lett ; 7(16): 3397-400, 2005 Aug 04.
Article in English | MEDLINE | ID: mdl-16048301

ABSTRACT

Hyperpolarized (129)Xe NMR spectroscopy is used to establish the solid-state porosity of shape-persistent macrocycles with either an organic or metal-organic framework. These studies show that even upon removal of cocrystallized solvent molecules, the macrocycles maintain a porous or channeled structure. The technique can provide valuable information about systems for which X-ray crystallographic analysis is not feasible. [structure: see text]

17.
J Am Chem Soc ; 126(35): 10972-80, 2004 Sep 08.
Article in English | MEDLINE | ID: mdl-15339183

ABSTRACT

The feasibility of (99)Ru NMR spectroscopy as a tool to characterize solid compounds is demonstrated. Results of the first solid-state (99)Ru NMR investigation of diamagnetic compounds are presented for Ru(NH(3))(6)Cl(2), K(4)Ru(CN)(6). xH(2)O (x = 0, 3), LaKRu(CN)(6), and Ru(3)(CO)(12). The sensitivity of the ruthenium magnetic shielding tensor to subtle changes in the local structure about the ruthenium nucleus is highlighted by comparing the (99)Ru isotropic chemical shift of Ru(NH(3))(6)Cl(2) in aqueous solutions and in the solid state. The narrow isotropic (99)Ru NMR peak observed for solid Ru(NH(3))(6)Cl(2) indicates that this compound is an ideal secondary reference sample for solid-state (99)Ru NMR studies. The isotropic (99)Ru chemical shift, (99)Ru nuclear quadrupolar coupling constant, C(Q), and quadrupolar asymmetry parameter of K(4)Ru(CN)(6). xH(2)O (x = 0, 3) are shown to be sensitive to x. For Ru(3)(CO)(12), the magnetic shielding tensors of each of the three nonequivalent Ru nuclei have spans of 1300-1400 ppm, and the (99)Ru C(Q) values are also similar, 1.36-1.85 MHz, and are surprisingly small given that (99)Ru has a moderate nuclear quadrupole moment. Information about the relative orientation of the Ru magnetic shielding and electric field gradient tensors has been determined for Ru(3)(CO)(12) from experimental (99)Ru NMR spectra as well as quantum chemical calculations.

18.
Chem Commun (Camb) ; (12): 1416-7, 2003 Jun 21.
Article in English | MEDLINE | ID: mdl-12841269

ABSTRACT

Depending on the details of the technique employed, desolvation of tBC inclusion compounds leads to either a dense, guest-free polymorph, or a guest-free low density polymorph, the latter also having distinct high and low temperature forms.

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