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1.
J Craniomaxillofac Surg ; 34 Suppl 2: 110-5, 2006 Sep.
Article in English | MEDLINE | ID: mdl-17071403

ABSTRACT

INTRODUCTION: Surgical correction of malocclusion changes the force to moment ratio of masticatory muscles inserting at the mandible caused by shortening, lengthening and rotation of the bone following osteotomy. During muscle adaptation the expression of mRNA for the myosin heavy chain (MyHC) of type I and type II fibres may be changed. MATERIAL AND METHODS: The adaptation of the masseter muscle was investigated at the mRNA level in 10 patients 6 months after orthognathic surgery in the mandible. The competitive polymerase chain reaction (cPCR) is a suitable method for quantification of MyHC mRNA. For application of this minimal invasive method an amount of 35 mg muscle tissue was sufficient. RESULTS: 6 month postoperatively there was a deficiency of about 87% of MyHC mRNA for fibre type I and II in both groups of patients. The deficiency in patients with mesial position of the mandible was higher but not significant different to patients with distal malocclusion. CONCLUSION: Patients should use the postoperative interval for training their masticatory muscles. This improves the stability of treatment result and prevents relapse.


Subject(s)
Mandible/surgery , Masseter Muscle/chemistry , Myosin Heavy Chains/analysis , Osteotomy/adverse effects , RNA, Messenger/analysis , Adult , Cephalometry , Female , Humans , Male , Malocclusion/surgery , Mandible/diagnostic imaging , Maxilla/diagnostic imaging , Myosin Heavy Chains/genetics , Radiography
2.
J Appl Genet ; 46(2): 227-36, 2005.
Article in English | MEDLINE | ID: mdl-15876691

ABSTRACT

The aim of this study was to determine the amount of myosin heavy chain (MyHC) proteins and MyHC mRNA in muscles of patients with different positions of the mandible. Ten adult patients for orthognathic surgery were divided into two groups: distal and mesial malocclusion. The mRNA expression of two MyHC isoforms of the anterior and posterior part of the right and left side of the human masseter muscle was analysed with a competitive RT-PCR assay. An exogenous template that includes oligonucleotide sequences specific for sarcomeric MyHC isoforms (1 and 2x) was constructed and utilized as competitor. Different isoforms of the MyHC protein were identified by Western blot analysis. In the total mRNA pool of the masseter muscle, the MyHC 1 mRNA level was 25.5 +/- 7.6% and the MyHC 2x mRNA was 2.5 +/- 1.2%. The anterior part of the masseter muscle from patients with distal occlusion contained more type 1 and 2x MyHC mRNA, as compared to patients with mesial occlusion (P < 0.05). No difference in the protein distribution was observed. The differences in mRNA expression may be caused by the enforced stress of the masticatory muscle in distal occlusion because of the disadvantageous pivot.


Subject(s)
Gene Expression Regulation , Malocclusion/genetics , Masseter Muscle/pathology , Myosin Heavy Chains/biosynthesis , Myosin Heavy Chains/genetics , Adult , Biomechanical Phenomena , Blotting, Western , Female , Humans , Male , RNA, Messenger/biosynthesis , Reverse Transcriptase Polymerase Chain Reaction , Stress, Mechanical
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