ABSTRACT
The bromine content of human gliomas and white matter was determined by neutron activation analysis (NAA) following p.o. administration of a single dose of 400-500 mg/m2 dibromodulcitol (DBD). In another group of patients with brain gliomas, the bromine content was measured subsequent to application of a single dose of 334 mg/m2 of sodium bromide (equivalent dose regarding the bromine content of DBD). The bromine content of these two groups was compared to the values found in a third control group of untreated patients. The amount of bromine after DBD application was three to four times higher than in the untreated samples and the average accumulation ratio of 1.8 +/- 0.4 proved to be nearly identical both in tumour and white matter. The bromine values after NaBr treatment showed a different pattern of distribution. The accumulation was higher in the tumour tissue than in the normal white matter. These findings demonstrate that the pharmacokinetic properties of DBD- and NaBr-derived bromine are different, suggesting that the increase of bromine after DBD administration could be due to covalently bound bromine in DBD.
Subject(s)
Brain Chemistry , Brain Neoplasms/analysis , Bromine/analysis , Glioblastoma/analysis , Mitolactol/metabolism , Humans , Neutron Activation AnalysisABSTRACT
The effect of l-noradrenaline and vanadate on the activity of Na+K+-activated ATPase was studied on synaptosomal brain cortex preparation. Using neutron activation analysis it was found that the rat cerebral cortex synaptosomal preparation contains 0.16 microM vanadium. The concentration of vanadium needed to reduce enzyme activity by 50% proved to be 2 x 10(-6) M. Evidence has been provided that the increase by noradrenaline of enzyme activity in synaptosomal preparation depends on the presence of an inhibitory contaminant in commercial ATP preparations. In homogenate, however, noradrenaline was able to enhance enzyme activity even when vanadium-free ATP was used. This fact indicates that noradrenaline removes the inhibitory effect of cytoplasmic factor thereby stimulating enzyme activity.
Subject(s)
Cerebral Cortex/enzymology , Norepinephrine/pharmacology , Sodium-Potassium-Exchanging ATPase/metabolism , Synaptosomes/enzymology , Vanadium/pharmacology , Animals , Diagnostic Errors , Rats , Sodium-Potassium-Exchanging ATPase/antagonists & inhibitorsABSTRACT
A total of 125 human lenses (97 senile cataractous and 28 clear) were analyzed by means of neutron activation analysis for trace element concentrations of copper, zinc, manganese, cobalt, rubidium, scandium, and nickel. Tt was found that the rubidium concentration remarkably decreased and the copper concentration moderately increased with the progression of cataract. The concentrations of manganese, zinc, and cobalt showed some fluctuations without a definite increase or decrease, and the scandium and nickel content was found to be very low.
Subject(s)
Cataract/metabolism , Lens, Crystalline/metabolism , Trace Elements/metabolism , Age Factors , Aged , Cobalt/metabolism , Copper/metabolism , Humans , Manganese/metabolism , Neutron Activation Analysis/methods , Nickel/metabolism , Rubidium/metabolism , Scandium/metabolism , Zinc/metabolismABSTRACT
Quantitative determination of the elements potassium, sodium, manganese, magnesium, iron, cobalt and zinc was performed in mycobacteria by neutron activation analysis. Mycobacterium phlei ATCC 19 249 at different phase of growth (4, 8, 13, 23 and 37 days old cultures), and 14 days old Mycobacterium bovis BCG cultures and uninoculated semi-synthetic Sauton culture media were examined. The elements studied could be divided into three groups; sodium, potassium and magnesium could be regarded as major, iron as minor, and zinc, manganese and cobalt as trace elements. M. phlei contained, with the exception of zinc, higher amounts of elements than M. bovis. Other metals (aluminium, antimony, rubidium) could also be detected.
