Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
Add more filters










Publication year range
1.
Int J Mol Sci ; 23(14)2022 Jul 14.
Article in English | MEDLINE | ID: mdl-35887127

ABSTRACT

The IgM and IgT classes were previously identified and characterized in the Antarctic teleost Trematomus bernacchii, a species belonging to the Perciform suborder Notothenoidei. Herein, we characterized the gene encoding the polymeric immunoglobulin receptor (pIgR) in the same species and compared it to the pIgR of multiple teleost species belonging to five perciform suborders, including 11 Antarctic and 1 non-Antarctic (Cottoperca gobio) notothenioid species, the latter living in the less-cold peri-Antarctic sea. Antarctic pIgR genes displayed particularly long introns marked by sites of transposable elements and transcription factors. Furthermore, analysis of T. bernacchii pIgR cDNA unveiled multiple amino acid substitutions unique to the Antarctic species, all introducing adaptive features, including N-glycosylation sequons. Interestingly, C. gobio shared most features with the other perciforms rather than with the cold-adapted relatives. T. bernacchii pIgR transcripts were predominantly expressed in mucosal tissues, as indicated by q-PCR and in situ hybridization analysis. These results suggest that in cold-adapted species, pIgR preserved its fundamental role in mucosal immune defense, although remarkable gene structure modifications occurred.


Subject(s)
Perciformes , Receptors, Polymeric Immunoglobulin , Animals , Antarctic Regions , DNA, Complementary/genetics , Perciformes/genetics , Phylogeny , Receptors, Polymeric Immunoglobulin/genetics
2.
Fish Shellfish Immunol ; 116: 124-139, 2021 Sep.
Article in English | MEDLINE | ID: mdl-34038801

ABSTRACT

Cryonotothenioidea is the main group of fishes that thrive in the extremely cold Antarctic environment, thanks to the acquisition of peculiar morphological, physiological and molecular adaptations. We have previously disclosed that IgM, the main immunoglobulin isotype in teleosts, display typical cold-adapted features. Recently, we have analyzed the gene encoding the heavy chain constant region (CH) of the IgT isotype from the Antarctic teleost Trematomus bernacchii (family Nototheniidae), characterized by the near-complete deletion of the CH2 domain. Here, we aimed to track the loss of the CH2 domain along notothenioid phylogeny and to identify its ancestral origins. To this end, we obtained the IgT gene sequences from several species belonging to the Antarctic families Nototheniidae, Bathydraconidae and Artedidraconidae. All species display a CH2 remnant of variable size, encoded by a short Cτ2 exon, which retains functional splicing sites and therefore is included in the mature transcript. We also considered representative species from the three non-Antarctic families: Eleginopsioidea (Eleginops maclovinus), Pseudaphritioidea (Pseudaphritis urvillii) and Bovichtidae (Bovichtus diacanthus and Cottoperca gobio). Even though only E. maclovinus, the sister taxa of Cryonotothenioidea, shared the partial loss of Cτ2, the other non-Antarctic notothenioid species displayed early molecular signatures of this event. These results shed light on the evolutionary path that underlies the origins of this remarkable gene structural modification.


Subject(s)
Fishes/genetics , Immunoglobulins/genetics , Animals , Antarctic Regions , DNA, Complementary/genetics , Evolution, Molecular , Exons , Fish Proteins , Head Kidney/immunology , Immunoglobulin Domains , Phylogeny , Spleen/immunology
3.
Biology (Basel) ; 10(2)2021 Feb 10.
Article in English | MEDLINE | ID: mdl-33578914

ABSTRACT

The vertebrate immune system provides a powerful defense because of the ability to potentially recognize an unlimited number of pathogens. The antibody molecule, also termed immunoglobulin (Ig) is one of the major mediators of the immune response. It is built up from two types of Ig domains: the variable domain, which provides the capability to recognize and bind a potentially infinite range of foreign substances, and the constant domains, which exert the effector functions. In the last 20 years, advances in our understanding of the molecular mechanisms and structural features of antibody in mammals and in a variety of other organisms have uncovered the underlying principles and complexity of this fundamental molecule. One notable evolutionary topic is the origin and evolution of antibody. Many aspects have been clearly stated, but some others remain limited or obscure. By considering a wide range of prokaryotic and eukaryotic organisms through a literature survey about the topic, we have provided an integrated view of the emergence of antibodies in evolution and underlined the very ancient origins.

4.
J Immunother ; 42(4): 97-109, 2019 05.
Article in English | MEDLINE | ID: mdl-30865026

ABSTRACT

Adoptive transfer of T lymphocytes (ACT) engineered with T-cell receptors (TCRs) of known antitumor specificity is an effective therapeutic strategy. However, a major constraint of ACT is the unpredictable interference of the endogenous TCR α and ß chains in pairing of the transduced TCR. This effect reduces the efficacy of the genetically modified primary T cells and carries the risk of generating novel TCR reactivities with unintended functional consequences. Here, we show a powerful approach to overcome these limitations. We engineered TCR α and ß chains with mutations encompassing a conserved motif (FXXXFXXS) required to stabilize the pairing of immunoglobulin heavy chain transmembrane domains. Molecular modeling supported the preferential pairing of mutated TCR and impaired pairing between mutated and wild-type TCRs. Expression of the mutated TCR was similar to wild type and conferred the expected specificity. Fluorescence resonance energy transfer analysis in mouse splenocytes transduced with mutated or wild-type TCRs showed a higher proximity of the former over the latter. Importantly, we show that mutated TCRs effectively outcompete endogenous TCRs and improve in vitro antitumor cytotoxicity when expressed in ex vivo isolated human T cells. This approach should contribute to improving current protocols of anticancer immunetherapy protocols.


Subject(s)
Protein Domains/genetics , Protein Interaction Domains and Motifs , Receptors, Antigen, T-Cell/genetics , Receptors, Antigen, T-Cell/metabolism , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Transduction, Genetic , Amino Acid Motifs , Amino Acid Sequence , Animals , Cell Line , Gene Expression , Genetic Therapy , Genetic Vectors , Humans , Immunotherapy, Adoptive , Lipid Bilayers/chemistry , Mice , Models, Molecular , Mutagenesis , Protein Conformation , Receptors, Antigen, T-Cell/chemistry , Receptors, Antigen, T-Cell, alpha-beta/chemistry , Receptors, Antigen, T-Cell, alpha-beta/genetics , Receptors, Antigen, T-Cell, alpha-beta/metabolism , Receptors, Chimeric Antigen/chemistry , Receptors, Chimeric Antigen/genetics , Receptors, Chimeric Antigen/metabolism , Structure-Activity Relationship , T-Cell Antigen Receptor Specificity/immunology
5.
Mar Genomics ; 24 Pt 1: 55-68, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26122835

ABSTRACT

Cloning and characterization of IgT heavy chain genes were performed in the Antarctic Notothenioid teleost Trematomus bernacchii and in a non-Antarctic Notothenioid species, Bovichtus diacanthus, belonging to a phyletically basal lineage of Notothenioids. Compared to IgT from other non-Antarctic teleost species, including B. diacanthus, T. bernacchii IgT lacked most of the second constant domain but maintained only a few amino acid residues, which could be aligned to B. diacanthus CH2 domain. By analyzing several cDNA clones from a single specimen, three differently sized IgT transcript variants, named Long, Short and Shortest, were identified. Genomic analysis of T. bernacchii and B. diacanthus IgH loci revealed that, in the case of T. bernacchii, within the intron between the exons coding for the entire first and second constant domains a reminiscence of the ancestral second exon was present. The Long and Short variants were found to be encoded by indel alleles, whereas the Shortest variant was generated by alternative splicing that led to the CH2 exonic remnant skipping. Through comparison between genomic and cDNA sequences we hypothesized the presence of three different copies of the IgT heavy chain gene, one of which being considered the functional gene since the corresponding transcripts were identified. Moreover, either Long or Short exonic variants were found to be used in IgT heavy chain membrane form in an unbiased manner, as seen for the secretory form. Phylogenetic analysis was performed on the constant region from all teleost IgT available to date, including IgT from another Antarctic Notothenioid species, Notothenia coriiceps, identified by searching the transcriptome. The loss of almost an entire domain together with the conservation of some amino acids such as proline, glycine and cysteine in the CH2 domain remnant, could be interpreted as another distinctive feature of the Antarctic fish genome evolution, providing also new insights into the structural variation of teleost immunoglobulin genes.


Subject(s)
Biological Evolution , Fishes/genetics , Fishes/metabolism , Gene Expression Regulation/immunology , Immunoglobulins/genetics , Immunoglobulins/metabolism , Amino Acid Sequence , Animals , Antarctic Regions , Base Sequence , DNA, Complementary/genetics , Fish Proteins , Genomics , Immunoglobulin Heavy Chains/genetics , Molecular Sequence Data , Phylogeny
6.
Mol Immunol ; 66(2): 299-309, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25909494

ABSTRACT

Notothenioidei are typical Antarctic teleosts evolved to adapt to the very low temperatures of the Antarctic seas. Aim of the present paper is to investigate sequence and structure of C3, the third component of the complement system of the notothenioid Trematomus bernacchii and Chionodraco hamatus. We determined the complete nucleotide sequence of two C3 isoforms of T. bernacchii and a single C3 isoform of C. hamatus. These sequences were aligned against other homologous teleost sequences to check for the presence of diversifying selection. Evidence for positive selection was observed in the evolutionary lineage of Antarctic teleost C3 sequences, especially in that of C. hamatus, the most recently diverged species. Adaptive selection affected numerous amino acid positions including three residues located in the anaphylatoxin domain. In an attempt to evaluate the link between sequence variants and specific structural features, we constructed molecular models of Antarctic teleost C3s, of their proteolytic fragments C3b and C3a, and of the corresponding molecules of the phylogenetically related temperate species Epinephelus coioides, using human crystallographic structures as templates. Subsequently, we compared dynamic features of these models by molecular dynamics simulations and found that the Antarctic C3s models show higher flexibility, which likely allows for more pronounced movements of both the TED domain in C3b and the carboxyl-terminal region of C3a. As such dynamic features are associated to positively selected sites, it appears that Antarctic teleost C3 molecules positively evolved toward an increased flexibility, to cope with low kinetic energy levels of the Antarctic marine environment.


Subject(s)
Anaphylatoxins/immunology , Complement C3/immunology , Evolution, Molecular , Fish Proteins/immunology , Perciformes/immunology , Phylogeny , Adaptation, Physiological/genetics , Adaptation, Physiological/immunology , Anaphylatoxins/chemistry , Anaphylatoxins/genetics , Animals , Antarctic Regions , Base Sequence , Cold Temperature , Complement C3/chemistry , Complement C3/genetics , Fish Proteins/chemistry , Fish Proteins/genetics , Gene Expression , Humans , Molecular Dynamics Simulation , Molecular Sequence Data , Perciformes/classification , Perciformes/genetics , Protein Isoforms/chemistry , Protein Isoforms/genetics , Protein Isoforms/immunology , Protein Structure, Tertiary , Proteolysis , Selection, Genetic , Sequence Alignment
7.
Fish Shellfish Immunol ; 39(2): 206-14, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24821425

ABSTRACT

The presence and production of IgM in the skin of the Antarctic teleost Trematomus bernacchii were investigated in this study. Immunoglobulins purified from cutaneous mucus and analysed by SDS-PAGE run under non-reducing and reducing conditions, were composed of heavy and light chains of 78 kDa and 25 kDa respectively, with a relative molecular mass of 830 kDa indicating that mucus IgM are tetramers as the serum IgM. Mature transcripts encoding the constant domains of both the secretory and membrane-bound Igµ chain were seen in T. bernacchii skin using a PCR strategy and the expression of the secretory Igµ chain in the skin was compared with that in other tissues by Real-time PCR. Cytological investigations revealed the presence of either immunoglobulins or their transcripts in occasional lymphocytes distributed close to the basal membrane. IgM once produced here, enters the filament-containing cells and is released into the mucus when these cells degenerate and detach from the epidermis. Our findings indicate that a cutaneous defence mechanism, functioning as anatomical and physiological barrier under subzero conditions, is present in this Antarctic species as an important component of the immune system.


Subject(s)
Acclimatization/immunology , Immunoglobulin M/immunology , Perciformes/immunology , Skin/immunology , Animals , Antarctic Regions , Cold Temperature , DNA Primers/genetics , Electrophoresis, Polyacrylamide Gel/veterinary , Immunohistochemistry/veterinary , In Situ Hybridization/veterinary , Lymphocytes/immunology , Real-Time Polymerase Chain Reaction/veterinary
8.
Biochim Biophys Acta ; 1828(11): 2637-45, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23896554

ABSTRACT

Plasma membrane lipids significantly affect assembly and activity of many signaling networks. The present work is aimed at analyzing, by molecular dynamics simulations, the structure and dynamics of the CD3 ζζ dimer in palmitoyl-oleoyl-phosphatidylcholine bilayer (POPC) and in POPC/cholesterol/sphingomyelin bilayer, which resembles the raft membrane microdomain supposed to be the site of the signal transducing machinery. Both POPC and raft-like environment produce significant alterations in structure and flexibility of the CD3 ζζ with respect to nuclear magnetic resonance (NMR) model: the dimer is more compact, its secondary structure is slightly less ordered, the arrangement of the Asp6 pair, which is important for binding to the Arg residue in the alpha chain of the T cell receptor (TCR), is stabilized by water molecules. Different interactions of charged residues with lipids at the lipid-cytoplasm boundary occur when the two environments are compared. Furthermore, in contrast to what is observed in POPC, in the raft-like environment correlated motions between transmembrane and cytoplasmic regions are observed. Altogether the data suggest that when the TCR complex resides in the raft domains, the CD3 ζζ dimer assumes a specific conformation probably necessary to the correct signal transduction.


Subject(s)
1,2-Dipalmitoylphosphatidylcholine/analogs & derivatives , CD3 Complex/chemistry , Lipid Bilayers/chemistry , Membrane Microdomains/chemistry , 1,2-Dipalmitoylphosphatidylcholine/chemistry , Amino Acid Sequence , Dimerization , Models, Molecular , Molecular Dynamics Simulation , Molecular Sequence Data , Sequence Homology, Amino Acid
9.
Mar Genomics ; 8: 43-8, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23199879

ABSTRACT

IgM represents the main immunoglobulin isotype shared by all teleost fish. However, Antarctic fish IgM possess a peculiar hinge region, which connects the CH2 and CH3 domains, not seen in any other teleost species. In the present study allelic polymorphism of IgM gene of the Antarctic teleost Trematomus benacchii was investigated. By nucleotide sequencing the entire Immunoglobulin heavy chain constant region from ten T. bernacchii individuals, 47 positions were found to be polymorphic. The largest number of polymorphic positions, accounting for 51% of the total, was found to fall within the hinge region. This region not only displayed extensive nucleotide variation, but also length diversity; in fact several sequences were one amino acid shorter as resulting from the usage of a different splice acceptor site of the CH3 exon, as demonstrated by genomic DNA analysis. The Ka/Ks ratios of the polymorphic positions showed typical values higher than 1, indicative of positive selection acting to polymorphic codons to favor amino acid replacements and maintain allelic variants.


Subject(s)
Alleles , Genes, Immunoglobulin Heavy Chain/genetics , Perciformes/genetics , Polymorphism, Genetic , Amino Acid Sequence , Amino Acid Substitution , Animals , Antarctic Regions , Base Sequence , Immunoglobulin M/genetics , Molecular Sequence Data , Sequence Alignment
10.
Fish Shellfish Immunol ; 33(5): 1076-85, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22960217

ABSTRACT

In the present study we address the investigation of TLR2 evolutionary selection in two Antarctic teleosts, Trematomus bernacchii (Nototheniidae) and Chionodraco hamatus (Channichthyidae). The nucleotide sequence of TLR2 has been determined in both species, encoding 20 leucine-rich repeats (LRRs) in the extracellular region and a classical Toll/IL-1R (TIR) domain in the intracellular region. High expression level of T. bernacchii TLR2 was found in spleen and skin. Using different methods we identified six codons that underwent Darwinian selection while 20 were found to be negatively selected. Molecular models of C. hamatus and T. bernacchii TLR2 ectodomain as well as of the TIR domain were built by Homology Modeling. Molecular Dynamics simulations were performed in water for 15 ns. The sites under positive selection were residing on the convex side of the solenoid, four out of six were in a 35-residue-long region including the central/N-terminal domain boundary: two in the external loop of LRR11 and the other two in the LRR12 loop. This region has been demonstrated to be the functional site of ligand interaction in human TLR2 structure. Antarctic TLR2 models showed more flexibility than TLR2 from the temperate species Gasterosteus aculeatus. These results suggest that the selective pressure has shaped TLR2 molecule in such a way that increased its activity under the peculiar Antarctic environmental conditions.


Subject(s)
Evolution, Molecular , Models, Molecular , Perciformes/genetics , Protein Conformation , Selection, Genetic , Toll-Like Receptor 2/genetics , Amino Acid Sequence , Animals , Antarctic Regions , Base Sequence , Computational Biology , Likelihood Functions , Models, Genetic , Molecular Dynamics Simulation , Molecular Sequence Data , Oligonucleotides/genetics , Phylogeny , Real-Time Polymerase Chain Reaction , Repetitive Sequences, Amino Acid/genetics , Sequence Analysis, DNA , Species Specificity , Toll-Like Receptor 2/chemistry
11.
Mar Genomics ; 5: 35-41, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22325720

ABSTRACT

Immunoglobulins (Ig) of Chondroichthyes have been extensively studied in sharks; in contrast, in skates investigations on Ig remain scarce and fragmentary despite the high occurrence of skates in all of the major oceans of the world. To focus on Rajidae Igµ, the most abundant heavy chain isotype, we have chosen the Antarctic species Bathyraja eatonii, Bathyraja albomaculata, Bathyraja brachyurops, and Amblyraja georgiana which live at high latitudes in the Southern Ocean, and at very low temperatures. We prepared mRNA from the spleen of individuals of each species and performed RT-PCR experiments using two oligonucleotides designed on the alignment of various elasmobranch Igµ heavy chain sequences available in GenBank. The PCR products, about 1400-nt long, were cloned and sequenced. Nucleotide sequence identities calculated for the constant region domains ranged from 88.5% to 97.5% between species, and from 91.1% to 99.7% within species. In a distance tree, including also Raja erinacea sequences, two major branches were obtained, one containing Arhynchobatinae sequences, the other one Rajinae sequences. Four presumptive D gene segments were identified in the region of the VH/D/JH recombination; two different D segments were often found in the same sequence. Moreover, 5-15 genomic fragments of different lengths, carrying the gene locus encoding Igµ chain were revealed by Southern blotting analysis. B. eatonii amino acid sequences were analyzed for the positional diversity by Shannon entropy analysis, showing CH4 as the most conserved domain, and CH3 as the most variable one. B. eatonii CDR3 region length varied between 11 and 15 amino acid residues; the mean length (13.4 aa) was greater than that of Leucoraja eglanteria sequences (7.7 aa). An alignment of representative sequences of Antarctic species and R. erinacea showed that more cysteine residues not involved in the intradomain disulfide bridges were present in Antarctic species.


Subject(s)
Fish Proteins/genetics , Immunoglobulin Heavy Chains/genetics , Skates, Fish/genetics , Amino Acid Sequence , Animals , Antarctic Regions , Base Sequence , Cloning, Molecular , Cysteine/genetics , Fish Proteins/immunology , Molecular Sequence Data , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNA , Sequence Analysis, Protein , Skates, Fish/immunology
12.
Fish Shellfish Immunol ; 31(5): 688-96, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21044686

ABSTRACT

We have investigated the immunoglobulin molecule and the genes encoding it in teleosts living in the Antarctic seas at the constant temperature of -1.86 °C. The majority of Antarctic teleosts belong to the suborder Notothenioidei (Perciformes), which includes only a few non-Antarctic species. Twenty-one Antarctic and two non-Antarctic Notothenioid species were included in our studies. We sequenced immunoglobulin light chains in two species and µ heavy chains, partially or totally, in twenty species. In the case of heavy chain, genomic DNA and the cDNA encoding the secreted and the membrane form were analyzed. From one species, Trematomus bernacchii, a spleen cDNA library was constructed to evaluate the diversity of VH gene segments. T. bernacchii IgM, purified from the serum and bile, was characterized. Homology Modelling and Molecular Dynamics were used to determine the molecular structure of T. bernacchii and Chionodraco hamatus immunoglobulin domains. This paper sums up the previous results and broadens them with the addition of unpublished data.


Subject(s)
Fish Diseases/immunology , Immunoglobulins/genetics , Immunoglobulins/immunology , Nematode Infections/veterinary , Perciformes/immunology , Amino Acid Sequence , Animals , Antarctic Regions , Antibodies, Helminth/blood , Base Sequence , Cell Membrane/metabolism , Cluster Analysis , Fish Diseases/parasitology , Genetic Variation , Immunoglobulin Heavy Chains/chemistry , Immunoglobulin Heavy Chains/genetics , Immunoglobulin Isotypes , Immunoglobulin Light Chains/chemistry , Immunoglobulin Light Chains/genetics , Immunoglobulins/chemistry , Models, Molecular , Molecular Sequence Data , Nematode Infections/immunology , Perciformes/classification , Perciformes/genetics , Phylogeny , Protein Structure, Tertiary , Sequence Alignment
13.
Mol Phylogenet Evol ; 57(3): 1238-44, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20937398

ABSTRACT

All species of vertebrates synthesize immunoglobulin molecules, which differ in an number of aspects but also share a few common features responsible for their function, such as the presence of a transmembrane domain in the membrane bound form of the immunoglobulin heavy chain (IgTMD) that ensures communication with the signal transducing Igα-Igß peptides. We have analyzed the gene sequence encoding the IgTMD of different heavy chain isotypes of very distant species, from shark to mammals. The IgTMD sequences show a high degree of sequence identity and their encoding nucleotide sequences were shown to be subject to purifying selection at most sites. We have built molecular models of seven IgTMDs from different vertebrate species and have investigated the formation of homodimer in a palmitoyl oleoyl phosphatidylcholine (POPC) lipid bilayer by molecular dynamics simulations. We found that the conserved FXXXFXXS/TXXXS motif, never observed to date in protein transmembrane chains, is responsible for the two heavy chains association through two pairs of Phe-Phe hydrophobic interactions and two pairs of Ser/Thr-Ser/Ser hydrogen bonds. This interaction pattern, which stabilizes the dimer conformation in the lipid bilayer, was unique, being different from any other pattern identified in transmembrane helices to date.


Subject(s)
Immunoglobulin Heavy Chains/genetics , Selection, Genetic , Amino Acid Sequence , Animals , B-Lymphocytes , Computer Simulation , Conserved Sequence , Models, Molecular , Molecular Sequence Data , Phylogeny , Protein Structure, Tertiary/genetics , Sequence Analysis, Protein , Vertebrates/genetics
14.
Mol Phylogenet Evol ; 55(1): 226-233, 2010 Apr.
Article in English | MEDLINE | ID: mdl-19800977

ABSTRACT

Notothenioid teleosts underwent major modifications of their genome to adapt to the cooling of the Antarctic environment. In order to identify specific features of the Antarctic teleost immunoglobulin, transcripts encoding the constant region of the IgM heavy chain from 13 Antarctic and non-Antarctic notothenioid species were sequenced. The primary mRNA splicing for the membrane form was found to be atypical in the majority of Antarctic species, because it led to exclusion of two entire constant exons, and to inclusion of 39-nucleotide exons encoding an unusually long Extracellular Membrane-Proximal Domain (EMPD). Genomic DNA analysis revealed that each 39-nucleotide exon fell within a long sequence that was the reverse complement of an upstream region. Deduced amino acid sequence analysis lead to the identification of cysteine encoding codons in the 39-nucleotide exons, but not in the respective sequence counterpart, suggesting that these residues might play an important role in the folding of the EMPD.


Subject(s)
Evolution, Molecular , Fishes/genetics , Immunoglobulin Heavy Chains/genetics , Animals , Base Sequence , Cold Temperature , Computational Biology , Exons , Fishes/classification , Genes, Immunoglobulin Heavy Chain , Immunoglobulin M/genetics , Models, Genetic , Models, Molecular , Molecular Sequence Data , RNA Splicing , Sequence Alignment , Sequence Analysis, DNA
15.
Mar Genomics ; 2(1): 19-25, 2009 Mar.
Article in English | MEDLINE | ID: mdl-21798168

ABSTRACT

We have chosen immunoglobulin, the key molecule of the immune system, to look for specific features accounting for adaptive evolution of Notothenioidei, the most abundant teleost species living in the Antarctic Ocean. Trematomus bernacchii immunoglobulin light chain was investigated and three isotypes were identified. Sequencing genomic DNA and transcripts encoding both the secreted and membrane-bound forms of the immunoglobulin heavy chain from 18 notothenioid species disclosed several unusual features. A limited diversity of the immunoglobulin heavy chain variable domain of the notothenioid teleost T. bernacchii was found, with only two VH gene families being defined. Analysis of deduced amino acid sequences of the secreted heavy chain showed an unexpected long hinge peptide, located at the CH2-CH3 boundary. To search for polymorphism, ten T. bernacchii individuals were analyzed: the largest number of polymorphic positions was found to fall within the hinge peptide suggesting that this feature may have some biological significance. In several species, alternative mRNA splicing that is responsible for the membrane-bound form synthesis generates heavy chains consisting of only two constant region domains. This domain-truncated form has a long extracellular spacer that has arisen from the insertion of multiple 39-nt repeats. Interestingly, each repeat was found to be the reverse complement of a sequence in the CH3 exon.

16.
J Mol Graph Model ; 27(3): 401-7, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18760646

ABSTRACT

The immune system cells express activating receptors, which consist of a dimeric ligand-binding molecule associated with a signal transducing dimer. The communication between the receptor partners depends primarily on the interactions between their membrane-embedded segments. In the B cell receptor (BCR) the sequence traversing the lipid bilayer of the immunoglobulin (IgTM) is highly conserved among species. We have investigated the association of the IgTM regions of the BCR of the Antarctic teleost Chionodraco hamatus. The nucleotide sequence of the entire immunoglobulin chain has been determined and the length, polarity, and structure of the IgTM region have been thoroughly analyzed. Structural models of the IgTM homodimer were also obtained by performing several MD simulations in a lipid bilayer using, as a starting model, two copies of the IgTM helix placed at various relative orientations and distances. Despite a certain degree of conformational heterogeneity, the predicted models of the IgTM homodimer display similar packing interfaces, characterized by a high degree of surface complementarity. The residues presumably responsible for the interaction and, consequently for the receptor stability have been identified in this manner.


Subject(s)
Cell Membrane/chemistry , Dimerization , Fishes , Immunoglobulins/chemistry , Amino Acid Sequence , Animals , Computer Simulation , Conserved Sequence , Models, Molecular , Molecular Sequence Data , Protein Structure, Secondary , Protein Structure, Tertiary , Sequence Alignment , Structural Homology, Protein , Thermodynamics
17.
Mol Immunol ; 45(11): 3096-106, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18455799

ABSTRACT

Three immunoglobulin light chain (IgL) isotypes TrbeL1, TrbeL2, and TrbeL3 were identified in the Antarctic teleost Trematomus bernacchii by immunoscreening a cDNA expression library, and using RT-PCR, and 5' RACE. One of them was distinguished in two subisotypes TrbeL1A and TrbeL1B. Real-time PCR experiments showed that the different isotypes were expressed in similar ratios in the various tissues analyzed. Interestingly, the expression level of TrbeL1A isotype was very high in all tissues. Molecular models of the CH1-CL domain pairings were built and minimized for the different isotypes. Several differences were identified in the superimposable structures mainly in the loops. In addition, the isotype-specific residues determined a different distribution of the charges on the external CL domain surface. Phylogenetic trees of 43 isotype representative sequences of CL domain from teleost species, built by different methods, indicated that all teleost light chain isotypes are distributed into three groups. Furthermore, the split of the group IgL1 into two subgroups, one of them carrying a micro-satellite DNA insertion, may have occurred in the Acanthopterygean ancestor.


Subject(s)
Immunoglobulin Isotypes/chemistry , Immunoglobulin Light Chains/chemistry , Perciformes/immunology , Amino Acid Sequence , Animals , Clone Cells , Gene Expression Profiling , Gene Library , Immunoglobulin Isotypes/genetics , Immunoglobulin Isotypes/immunology , Immunoglobulin Light Chains/genetics , Immunoglobulin Light Chains/immunology , Models, Molecular , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Protein Structure, Secondary , Protein Structure, Tertiary , Sequence Alignment , Sequence Analysis, Protein , Sequence Homology, Amino Acid , Static Electricity
18.
Genetica ; 130(1): 9-17, 2007 May.
Article in English | MEDLINE | ID: mdl-16909334

ABSTRACT

The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.


Subject(s)
Chromosome Mapping , Genes, Immunoglobulin Heavy Chain , In Situ Hybridization, Fluorescence , Perciformes/genetics , Animals , Antarctic Regions , DNA, Complementary/analysis , Perciformes/immunology , Phylogeny
19.
Dev Comp Immunol ; 29(5): 431-42, 2005.
Article in English | MEDLINE | ID: mdl-15707664

ABSTRACT

Purified Trematomus bernacchii bile IgM analysed by SDS-PAGE under reducing and non-reducing conditions consisted essentially of tetramers of the basic structure H2L2. The relative molecular mass of the glycosilated H chain was 76 kDa, while that of L chain was 25 kDa. In addition, the presence in the liver of IgM and mu chain-specific mRNA was demonstrated. Immunohistochemistry detected IgH- and IgL-reactivity in perisinusoidal cells, bile canaliculi and pre-ductules. In the anterior intestine, the intraluminal mucus retained a significant Ig-immunoreactivity, while the mucosa housed a limited density of Ig-producing cells. These findings strongly indicate that Ig could be transported across the hepatocytes to be secreted into the bile and protect the intestinal epithelium. In addition, extravasated plasma cells accumulated within liver portal tracts and close to the capsule that, in turn, was evenly coated by Ig molecules at the peritoneal surface.


Subject(s)
Biliary Tract/metabolism , Fishes/immunology , Immunoglobulin M/metabolism , Liver/metabolism , Animals , Bile/immunology , Bile/metabolism , Biliary Tract/immunology , Fishes/metabolism , Immune Sera/immunology , Immunoglobulin M/immunology , Immunohistochemistry , Intestine, Small/immunology , Intestine, Small/metabolism , Liver/immunology , Protein Transport/immunology , Protein Transport/physiology , Rabbits
20.
Comp Biochem Physiol B Biochem Mol Biol ; 135(2): 349-57, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12798944

ABSTRACT

Trematomus bernacchii immunoglobulin M concentration was determined in the serum by ELISA; the mean concentration value was 2.7 mg/ml corresponding to 9.6% of the total serum proteins. Purified IgM was analyzed by SDS-polyacrylamide gel electrophoresis, isoelectrofocusing and 2D electrophoresis. The relative molecular mass of the polymeric form was 830 kDa; that of separated H and L chains was, respectively, 78 and 25 kDa. The isoelectric points of the entire molecule ranged from 4.4 to 6.5, that of isolated H chains was between 4.0 and 6.0. Separated H chains were shown to reaggregate in tetrameric form. The cleavage site of trypsin was at the end of the CH1 domain, as confirmed by the N-terminal amino acid sequence of one of the resultant peptides. Immunoblotting was used to detect carbohydrates in the H and L chains labeled with digoxigenin. Glycosyl residues were detected only in the H chain. The carbohydrate content was evaluated to be 12.8% of the entire chain. Purified Igs were hydrolyzed by N-glycosidase F at different conditions and at least four different hydrolytic sites were revealed by limited deglycosylation. T. bernacchii IgM was also compared to those of five other polar fish species.


Subject(s)
Immunoglobulin M/blood , Immunoglobulin M/chemistry , Perciformes/immunology , Animals , Antarctic Regions , Carbohydrates/analysis , Electrophoresis, Polyacrylamide Gel , Immunoglobulin M/isolation & purification , Trypsin/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...