Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 39
Filter
Add more filters










Publication year range
1.
J Clin Invest ; 108(2): 289-301, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11457882

ABSTRACT

Podocalyxin (PC), the major sialoprotein of glomerular epithelial cells (GECs), helps maintain the characteristic architecture of the foot processes and the patency of the filtration slits. PC associates with actin via ezrin, a member of the ERM family of cytoskeletal linker proteins. Here we show that PC is linked to ezrin and the actin cytoskeleton via Na(+)/H(+)-exchanger regulatory factor 2 (NHERF2), a scaffold protein containing two PDZ (PSD-95/Dlg/ZO-1) domains and an ERM-binding region. The cytoplasmic tail of PC contains a C-terminal PDZ-binding motif (DTHL) that binds to the second PDZ domain of NHERF2 in yeast two-hybrid and in vitro pull-down assays. By immunocytochemistry NHERF2 colocalizes with PC and ezrin along the apical domain of the GEC plasma membrane. NHERF2 and ezrin form a multimeric complex with PC, as they coimmunoprecipitate with PC. The PC/NHERF2/ezrin complex interacts with the actin cytoskeleton, and this interaction is disrupted in GECs from puromycin aminonucleoside-, protamine sulfate-, or sialidase-treated rats, which show a dramatic loss of foot processes, comparable to that seen in the nephrotic syndrome. Thus NHERF2 appears to function as a scaffold protein linking PC to ezrin and the actin cytoskeleton. PC/NHERF2/ezrin/actin interactions are disrupted in pathologic conditions associated with changes in GEC foot processes, indicating their importance for maintaining the unique organization of this epithelium.


Subject(s)
Actins/metabolism , Kidney Glomerulus/metabolism , Sialoglycoproteins/metabolism , Actins/analysis , Actins/chemistry , Animals , Binding Sites , Cell Membrane/metabolism , Cells, Cultured , Cytoskeletal Proteins , Cytoskeleton/chemistry , Cytoskeleton/metabolism , DNA, Complementary/analysis , Epithelial Cells/metabolism , Immunohistochemistry , Kidney Glomerulus/pathology , Kidney Glomerulus/ultrastructure , Male , Molecular Sequence Data , Nephrotic Syndrome/metabolism , Nephrotic Syndrome/pathology , Phosphoproteins/analysis , Phosphoproteins/chemistry , Phosphoproteins/genetics , Rats , Rats, Sprague-Dawley , Sialoglycoproteins/analysis , Sialoglycoproteins/chemistry , Sodium-Hydrogen Exchangers/chemistry , Sodium-Hydrogen Exchangers/metabolism
2.
Mol Biol Cell ; 12(5): 1467-79, 2001 May.
Article in English | MEDLINE | ID: mdl-11359936

ABSTRACT

Low-density lipoprotein receptor-related protein (LRP) mediates internalization of urokinase:plasminogen activator inhibitor complexes (uPA:PAI-1) and the urokinase receptor (uPAR). Here we investigated whether direct interaction between uPAR, a glycosyl-phosphatidylinositol-anchored protein, and LRP, a transmembrane receptor, is required for clearance of uPA:PAI-1, regeneration of unoccupied uPAR, activation of plasminogen, and the ability of HT1080 cells to invade extracellular matrix. We found that in the absence of uPA:PAI-1, uPAR is randomly distributed along the plasma membrane, whereas uPA:PAI-1 promotes formation of uPAR-LRP complexes and initiates redistribution of occupied uPAR to clathrin-coated pits. uPAR-LRP complexes are endocytosed via clathrin-coated vesicles and traffic together to early endosomes (EE) because they can be coimmunoprecipitated from immunoisolated EE, and internalization is blocked by depletion of intracellular K(+). Direct binding of domain 3 (D3) of uPAR to LRP is required for clearance of uPA-PAI-1-occupied uPAR because internalization is blocked by incubation with recombinant D3. Moreover, uPA-dependent plasmin generation and the ability of HT1080 cells to migrate through Matrigel-coated invasion chambers are also inhibited in the presence of D3. These results demonstrate that GPI-anchored uPAR is endocytosed by piggybacking on LRP and that direct binding of occupied uPAR to LRP is essential for internalization of occupied uPAR, regeneration of unoccupied uPAR, plasmin generation, and invasion and migration through extracellular matrix.


Subject(s)
Cell Membrane/metabolism , Endocytosis , Plasminogen Activators/metabolism , Receptors, Cell Surface/metabolism , Receptors, Immunologic/metabolism , Urokinase-Type Plasminogen Activator/metabolism , Cell Line , Cell Movement/drug effects , Coated Pits, Cell-Membrane/metabolism , Endocytosis/drug effects , Fibrosarcoma , Humans , Low Density Lipoprotein Receptor-Related Protein-1 , Microscopy, Fluorescence , Models, Biological , Precipitin Tests , Protein Binding , Protein Structure, Tertiary , Protein Transport , Receptors, Urokinase Plasminogen Activator , Recombinant Proteins/metabolism , Tumor Cells, Cultured
3.
Exp Cell Res ; 260(2): 199-207, 2000 Nov 01.
Article in English | MEDLINE | ID: mdl-11035914

ABSTRACT

The receptor-associated protein (RAP) is a chaperone found primarily in the endoplasmic reticulum (ER) that plays a necessary role in the folding and exocytic trafficking of members of the LDL receptor gene family including megalin and the LDL receptor-related protein (LRP). Recently, RAP has been shown to interact with a growing number of proteins including several that are unrelated to the LDL receptor family as well as new members of this rapidly expanding family. Based on these observations, we have applied chemical crosslinking procedures to identify additional novel RAP-interacting proteins, and thereby better characterize the scope of RAP's ER-related function. In this study, we have identified eight proteins with molecular weights of 32, 35, 46, 55, 70, 95, 170, and 200 kDa that interact with endogenous RAP. These proteins were found to associate with RAP in multiple cell types from different species, suggesting that their expression and interactions with RAP are ubiquitous. Results of pulse-chase experiments show that most of the proteins remain sensitive to endoglycosidase-H digestion, and also remain stably associated with RAP over an extended period, suggesting that they are ER resident proteins. All of the RAP-associated proteins appear to be largely soluble as they partition into the aqueous phase following TX-114 detergent extraction. Sequence analysis and immunoblotting of the 46-kDa RAP-associated glycoprotein (gp46) shows that it is structurally and immunologically related to actin. If gp46 is also functionally related to actin as an intracellular structural protein, it may represent a novel component of the putative ER matrix.


Subject(s)
Actins/metabolism , Endoplasmic Reticulum/metabolism , Glycoproteins/metabolism , Membrane Glycoproteins/metabolism , Receptors, LDL/metabolism , Animals , Heymann Nephritis Antigenic Complex , Humans , Membrane Proteins/metabolism , Mice , Rats , Time Factors , Tumor Cells, Cultured
4.
Mol Biol Cell ; 11(9): 3219-32, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10982412

ABSTRACT

Podocalyxin is a major membrane protein of the glomerular epithelium and is thought to be involved in maintenance of the architecture of the foot processes and filtration slits characteristic of this unique epithelium by virtue of its high negative charge. However, until now there has been no direct evidence for podocalyxin's function. Podocalyxin is a type 1 transmembrane sialoprotein with an N-terminal mucin-like domain. To assess its function, we cloned rat podocalyxin and examined the effects of its expression on the cell adhesion properties of stably transfected Chinese hamster ovary (CHO)-K1 and Madin-Darby canine kidney (MDCK) cells and inducible ecdysone receptor-expressing (EcR)-CHO cells. In a cell aggregation assay, CHO-K1 cells expressing high levels of podocalyxin showed complete inhibition of cell aggregation, and MDCK transfectants showed greatly reduced aggregation ( approximately 60-80%) compared with parental cells. In EcR-CHO cells, the expression level of podocalyxin induced by increasing levels of ecdysone analogue correlated closely with the antiadhesion effect. The inhibitory effect of podocalyxin was reversed by treatment of the cells with Arthrobacter ureafaciens sialidase, indicating that sialic acid is required for inhibition of cell adhesion. Overexpression of podocalyxin also affected transepithelial resistance and the distribution of junctional proteins in MDCK cells by an unknown mechanism that may involve interaction with the actin cytoskeleton. These results provide direct evidence that podocalyxin functions as an antiadhesin that maintains an open filtration pathway between neighboring foot processes in the glomerular epithelium by charge repulsion.


Subject(s)
Cell Adhesion/physiology , Intercellular Junctions/physiology , Sialoglycoproteins/physiology , Amino Acid Sequence , Animals , Arthrobacter/enzymology , CHO Cells , Cell Aggregation/physiology , Cell Line , Chickens , Cloning, Molecular , Cricetinae , Dogs , Glycosylation , Humans , Kidney , Molecular Sequence Data , Neuraminidase/metabolism , Phosphorylation , Rabbits , Rats , Receptors, Steroid/genetics , Receptors, Steroid/physiology , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Sialoglycoproteins/chemistry , Sialoglycoproteins/genetics , Transfection
5.
J Am Soc Nephrol ; 9(10): 1759-66, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9773776

ABSTRACT

Renal clearance is a major pathway for regulating the levels of insulin and other low molecular weight polypeptide hormones in the systemic circulation. Previous studies have shown that the reabsorption of insulin from the glomerular filtrate occurs by binding to as yet unidentified sites on the luminal surface of proximal tubule cells followed by endocytosis and degradation in lysosomes. In this study, an insulin binding site was identified in renal microvillar membranes by chemical cross-linking procedures. By immunoprecipitation it was demonstrated that this binding site is megalin, the large multiligand binding endocytic receptor that is abundantly expressed in clathrin-coated pits on the apical surface of proximal tubule cells. Moreover, using cytochemical procedures, it was also shown that megalin is able to internalize insulin into endocytic vesicles. In ligand blotting assays, megalin also bound several other low molecular weight polypeptides, including beta2-microglobulin, epidermal growth factor, prolactin, lysozyme, and cytochrome c. These data suggest that megalin may play a significant role as a renal reabsorption receptor for the uptake of insulin and other low molecular weight polypeptides from the glomerular filtrate.


Subject(s)
Insulin/metabolism , Kidney Glomerulus/metabolism , Membrane Glycoproteins/metabolism , Animals , Binding Sites/physiology , Cell Membrane/physiology , Cells, Cultured , Endothelium, Vascular/metabolism , Heymann Nephritis Antigenic Complex , Immunoblotting , Immunoglobulin G/metabolism , Kidney Glomerulus/immunology , Microvilli/metabolism , Molecular Weight , Precipitin Tests , Rats , Sensitivity and Specificity
6.
J Am Soc Nephrol ; 9(9): 1638-44, 1998 Sep.
Article in English | MEDLINE | ID: mdl-9727372

ABSTRACT

Megalin (gp330) is the main target antigen involved in the induction of Heymann nephritis (HN), a rat model of human membranous nephropathy. Its large extracellular region contains four putative ligand-binding domains separated by spacer regions. Previously, it was reported that the second ligand-binding domain (LBD II) of megalin is involved in the pathogenesis of passive HN because it is capable of binding antibodies in vivo and initiating formation of immune deposits (ID). This study explores the possibility that pathogenic epitopes might also be present in the other putative ligand-binding domains. Recombinant fragments of ligand-binding domains (LBD) I through IV expressed in a baculovirus system were used to generate polyclonal domain-specific antibodies. Antibodies raised against each of the recombinant megalin fragments reacted preferentially with its respective antigen and with whole megalin by immunoblotting. Each of the antibodies also gave a characteristic brush-border staining for megalin by indirect immunofluorescence on rat kidney. When rats were injected with the domain-specific antibodies to test their ability to produce passive HN, glomerular ID were present in kidneys of all injected animals. The staining pattern in glomeruli of rats injected with LBD I, III, or IV was similar to that obtained with antibodies to LBD II. It is concluded that passive HN can be induced with antibodies against LBD I, III, and IV, as well as LBD II, and that each of the ligand-binding domains contains a pathogenic epitope. These findings provide further evidence for the multiple epitope model of HN.


Subject(s)
DNA, Complementary/analysis , Epitopes/analysis , Glomerulonephritis/immunology , Kidney Glomerulus/immunology , Membrane Glycoproteins/immunology , Animals , Binding Sites, Antibody , Disease Models, Animal , Epitopes/genetics , Female , Fluorescent Antibody Technique, Indirect , Gene Expression , Glomerulonephritis/genetics , Heymann Nephritis Antigenic Complex , Humans , Immunoblotting , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/genetics , Molecular Sequence Data , Rats , Rats, Sprague-Dawley , Recombinant Proteins , Sequence Homology, Amino Acid
7.
J Clin Invest ; 100(9): 2303-9, 1997 Nov 01.
Article in English | MEDLINE | ID: mdl-9410908

ABSTRACT

Megalin/gp330 is an endocytic receptor that internalizes multiple ligands including apolipoproteins E (apo E) and B100 (apo B). Megalin is the main antigenic target in passive Heymann nephritis (pHN), where it binds circulating autoantibodies leading to the formation of subepithelial immune deposits (ID)-the hallmark of pHN. Apo E and apo B were found recently to accumulate within these IDs, and evidence was provided that their lipids may undergo peroxidation, causing glomerular basement membrane damage and proteinuria. Here we investigated if ID-forming antimegalin IgG can inhibit the binding and internalization of apo E-betaVLDL (very low density lipoprotein) by megalin, and lead to their accumulation within IDs. By immunoelectron microscopy, apo E and apo B were detected in clathrin-coated pits and multivesicular bodies of podocytes in control rats, suggesting that the uptake of lipoproteins is a constitutive function of the glomerular epithelium. When pHN was induced by intravenous injection of antimegalin IgG, apo E and apo B were found within IDs by immunofluorescence and immunoelectron microscopy. Bound antibodies eluted from glomeruli of rats with pHN were found to inhibit the binding and internalization of apo E-enriched betaVLDL by megalin. These results indicate that pHN-inducing antimegalin IgG is capable of interfering with the uptake of lipoproteins by megalin in vivo during the formation of IDs.


Subject(s)
Apolipoproteins/metabolism , Glomerulonephritis/metabolism , Membrane Glycoproteins/metabolism , Animals , Antigen-Antibody Complex/metabolism , Apolipoproteins B/metabolism , Apolipoproteins E/metabolism , Autoantibodies/immunology , Autoantigens/metabolism , Endocytosis , Fluorescent Antibody Technique, Indirect , Glomerulonephritis/immunology , Heymann Nephritis Antigenic Complex , Immunization, Passive , Immunohistochemistry , Kidney/metabolism , Lipoproteins, VLDL/metabolism , Male , Membrane Glycoproteins/immunology , Rats , Rats, Sprague-Dawley , Receptors, LDL/metabolism
8.
Mol Biol Cell ; 8(3): 517-32, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9188102

ABSTRACT

Megalin (gp330) is a member of the low-density lipoprotein receptor gene family. Like other members of the family, it is an endocytic receptor that binds a number of specific ligands. Megalin also binds the receptor-associated protein (RAP) that serves as an exocytic traffic chaperone and inhibits ligand binding to the receptor. To investigate the fate of megalin/RAP complexes, we bound RAP glutathione-S-transferase fusion protein (RAP-GST) to megalin at the surface of L2 yolk sac carcinoma cells and followed the trafficking of the complexes by immunofluorescence and immunogold labeling and by their distribution on Percoll gradients. We show that megalin/RAP-GST complexes, which are internalized via clathrin-coated pits, are delivered to early endosomes where they accumulate during an 18 degrees C temperature block and colocalize with transferrin and transferrin receptor. Upon release from the temperature block, the complexes travel to late endosomes where they colocalize with rab7 and can be coprecipitated with anti-RAP-GST antibodies. Dissociation of the complex occurs in late endosomes and is most likely triggered by the low pH (approximately 5.5) of this compartment. RAP is then rapidly delivered to lysosomes and degraded whereas megalin is recycled to the cell surface. When the ligand, lipoprotein lipase, was bound to megalin, the receptor was found to recycle through early endosomes. We conclude that in contrast to receptor/ligand complexes, megalin/RAP complexes traffic through late endosomes, which is a novelty for members of the low-density lipoprotein receptor gene family.


Subject(s)
Carrier Proteins/metabolism , Endocytosis , Endosomes/metabolism , Glycoproteins/metabolism , Membrane Glycoproteins/metabolism , Animals , Autoantigens/immunology , Autoantigens/metabolism , Carrier Proteins/immunology , Cell Compartmentation , Endosomes/physiology , Endosomes/ultrastructure , Glycoproteins/immunology , Heymann Nephritis Antigenic Complex , Immunoglobulin Fab Fragments/metabolism , Immunoglobulin Fab Fragments/physiology , Kidney Glomerulus/immunology , LDL-Receptor Related Protein-Associated Protein , Lysosomes/metabolism , Lysosomes/ultrastructure , Membrane Glycoproteins/immunology , Membrane Proteins/metabolism , Molecular Chaperones , Protein Binding , Rats , Receptors, LDL/metabolism
9.
Proc Natl Acad Sci U S A ; 94(6): 2368-73, 1997 Mar 18.
Article in English | MEDLINE | ID: mdl-9122201

ABSTRACT

Megalin is a large cell surface receptor that mediates the binding and internalization of a number of structurally and functionally distinct ligands from the lipoprotein and protease:protease inhibitor families. To begin to address how megalin is able to bind ligands with unique structurally properties, we have mapped a binding site for apolipoprotein E (apoE)-beta very low density lipoprotein (beta VLDL), lipoprotein lipase, aprotinin, lactoferrin, and the receptor-associated protein (RAP) within the primary sequence of the receptor. RAP is known to inhibit the binding of all ligands to megalin. We identified a ligand-binding site on megalin by raising mAb against purified megalin, selected for a mAb whose binding to megalin is inhibited by RAP, and mapped the epitope for this mAb. mAb AC10 inhibited the binding of apoE-beta VLDL, lipoprotein lipase, aprotinin, and lactoferrin to megalin in a concentration-dependent manner. When cDNA fragments encoding the four cysteine-rich ligand-binding repeats in megalin were expressed in a baculovirus system and immunoblotted with AC10, it recognized only the second cluster of ligand-binding repeats. The location of the epitope recognized by mAb AC10 within this domain was pinpointed to amino acids 1111-1210. From these studies we conclude that the binding of apoE-beta VLDL, lactoferrin, aprotinin, lipoprotein lipase, and RAP to megalin is either competitively or sterically inhibited by mAb AC10 suggesting that these ligands bind to the same or closely overlapping sites within the second cluster of ligand-binding repeats.


Subject(s)
Kidney/metabolism , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/metabolism , Microvilli/metabolism , Receptors, LDL/metabolism , Animals , Antibodies, Monoclonal , Binding Sites , Binding, Competitive , Carrier Proteins/metabolism , Cysteine , Glycoproteins/metabolism , Heymann Nephritis Antigenic Complex , Immunoblotting , Kidney/immunology , Kinetics , LDL-Receptor Related Protein-Associated Protein , Ligands , Membrane Glycoproteins/isolation & purification , Models, Structural , Protein Conformation , Rats , Receptors, LDL/chemistry
10.
Brain Res ; 740(1-2): 6-14, 1996 Nov 18.
Article in English | MEDLINE | ID: mdl-8973792

ABSTRACT

The secreted isoforms of the amyloid precursor protein (APP) that contain the Kunitz domain are also known as protease nexin 2 (PN2). Normal proteolytic processing of transmembrane APP, which results in the majority of soluble PN2, cleaves within the Alzheimer's A beta peptide, precluding A beta formation. Recent data indicate that soluble PN2 is internalized by cells via the low density lipoprotein receptor-related protein (LRP), which binds multiple ligands including apolipoprotein E (apoE) [23]. However, soluble PN2 cannot contribute to amyloid accumulation, so we examined whether the unprocessed, transmembrane form of APP751 containing the intact A beta sequence would form complexes with a PN2 ligand, EGF binding protein (EGFBP), and be internalized by LRP. We found that the addition of EGFBP to cells overexpressing APP751 induced the internalization of this amyloidogenic form of APP. The 39 kDa LRP receptor associated protein (RAP), an antagonist for LRP, blocked the internalization of APP751/PN2, suggesting a common LRP-mediated internalization pathway for both soluble and transmembrane APP751/PN2 after protease complex formation. Previous work has shown that internalization of transmembrane APP can lead to the formation of amyloidogenic carboxyl-terminal fragments and increased secretion of the Alzheimer's A beta peptide. Our data suggest the protease ligands for PN2 may play an important role in altering APP processing pathways to favor amyloid formation, and that LRP may be a point at which the apoE and amyloid processing pathways intersect.


Subject(s)
Amyloid beta-Protein Precursor/metabolism , Carrier Proteins/metabolism , Fibroblasts/metabolism , Receptors, LDL/metabolism , Binding, Competitive , Humans , Protease Nexins , Receptors, Cell Surface
11.
Proc Natl Acad Sci U S A ; 93(16): 8601-5, 1996 Aug 06.
Article in English | MEDLINE | ID: mdl-8710917

ABSTRACT

Megalin (gp330), an epithelial endocytic receptor, is a major target antigen of Heymann nephritis (HN), an autoimmune disease in rats. To elucidate the mechanisms of HN, we have mapped a pathogenic epitope in megalin that binds anti-megalin antibodies. We focused our attention on four clusters of cysteine-rich, low density lipoprotein receptor (LDLR) ligand binding repeats in the extracellular domain of megalin because they represent putative ligand binding regions and therefore would be expected to be exposed in vivo and to be able to bind circulating antibodies. Rat megalin cDNA fragments I through IV encoding the first through fourth clusters of ligand-binding repeats, respectively, were expressed in a baculovirus system. All four expression products were detected by immunoblotting with two antisera capable of inducing passive HN (pHN). When antibodies eluted from glomeruli of rats with pHN were used for immunoblotting, only the expression product encoded by fragment II was detected. This indicates that the second cluster of LDLR ligand binding repeats is directly involved in binding anti-megalin antibodies and in the induction of pHN. To narrow the major epitope in this domain, fragment II was used to prepare proteins sequentially truncated from the C- and N-terminal ends by in vitro translation. Analysis of the truncated translation products by immunoprecipitation with anti-megalin IgG revealed that the fifth ligand-binding repeat (amino acids 1160-1205) contains the major epitope recognized. This suggests that a 46-amino acid sequence in the second cluster of LDLR ligand binding repeats contains a major pathogenic epitope that plays a key role in pHN. Identification of this epitope will facilitate studies on the pathogenesis of HN.


Subject(s)
Autoantigens/chemistry , Glomerulonephritis/immunology , Membrane Glycoproteins/immunology , Amino Acid Sequence , Animals , Base Sequence , Binding Sites , DNA Primers/chemistry , Epitope Mapping , Epitopes/chemistry , Heymann Nephritis Antigenic Complex , Membrane Glycoproteins/chemistry , Molecular Sequence Data , Nucleopolyhedroviruses , Rats , Receptors, LDL/chemistry , Receptors, LDL/immunology , Repetitive Sequences, Nucleic Acid , Spodoptera
12.
J Exp Med ; 183(5): 2007-15, 1996 May 01.
Article in English | MEDLINE | ID: mdl-8642311

ABSTRACT

Passive Heymann nephritis (pHN) is an experimental rat model for human membranous glomerulopathy. In pHN, the formation of subepithelial immune deposits (ID) involves as antigenic targets the membrane glycoprotein gp330/megalin and the 44-kD receptor-associated protein (RAP). A single binding site for ID- inducing antibodies (Abs) was previously mapped to the 86 NH2-terminal amino acids of RAP (RAP1-86). To further narrow this epitope, Abs eluted from the glomeruli were immunoblotted on membranes that were loaded with overlapping synthetic peptides representing the amino acid sequence of RAP (SPOTs system). Two adjacent Ab-binding domains with the sequences PVRLAF, (amino acids 39-44) and HSD-LKIQE (amino acids 46-53), which were separated by a single L residue at amino acid 45, were detected. Rabbit Abs raised against synthetic peptides containing these domains individually (P31-44 and P46-53) failed to procedure glomerular IDs. By contrast, Abs raised against a larger composite peptide (P31-53) induced IDs within 3d that were firmly cross linked to the glomerular basement membrane. These data suggest that Ab binding in vivo depends on the conformation of the antigenic target sequence that is preserved in the synthetic peptide P31-53, which covers the entire Ab-binding domain of RAP but not in its subdomains, P31-44 and P46-53. Collectively, these results locate the sole ID-inducing epitope of RAP to amino acids 39-53.


Subject(s)
Carrier Proteins/immunology , Glomerulonephritis/immunology , Glycoproteins/immunology , Kidney Glomerulus/immunology , Membrane Glycoproteins/immunology , Amino Acid Sequence , Animals , Antibody Formation , Antibody Specificity , Autoantigens/immunology , Carrier Proteins/chemistry , Coated Pits, Cell-Membrane/immunology , Coated Pits, Cell-Membrane/pathology , Epitopes/immunology , Glomerulonephritis/pathology , Glycoproteins/chemistry , Heymann Nephritis Antigenic Complex , Histocompatibility Antigens Class II/immunology , Humans , Immunoglobulin G , Kidney Glomerulus/pathology , LDL-Receptor Related Protein-Associated Protein , Male , Microscopy, Immunoelectron , Models, Structural , Molecular Chaperones/immunology , Molecular Sequence Data , Protein Structure, Secondary , Rabbits , Rats , Rats, Inbred Lew , Sequence Homology, Amino Acid
13.
J Am Soc Nephrol ; 6(1): 35-47, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7579068

ABSTRACT

Heymann nephritis (HN) has been extensively studied as a model of human membranous nephropathy since it was first described by Heymann in 1959. HN was induced in active form by the immunization of rats with antigens derived from the proximal tubule brush border, resulting in subepithelial glomerular immune deposits. HN was also induced passively by the injection of antibrush border antibodies into normal rats. A breakthrough in the understanding of the pathogenesis of HN was made in the 1970s, when it was established that the disease was due to the binding of circulating antibodies to glomerular components. This in turn led to a search to identify the endogenous antigen(s). In 1982, gp330 (now called megalin), a glycoprotein located in clathrin-coated pits of glomerular and proximal tubular epithelia, was identified as a target antigen. In 1990, a second protein (44 kd), now known as RAP (for receptor associated protein), that binds to megalin was also shown to be a target antigen. Both molecules have been cloned and sequenced, and their role in normal epithelial cells has been explored. It has come to light that megalin (gp330) is a member of the low-density lipoprotein receptor gene family and functions as a multiligand receptor for the uptake of a variety of macromolecules (plasminogen, protease: protease inhibitor complexes, apolipoprotein E-enriched very low-density lipoproteins, lactoferrin, among others). RAP associates with megalin and appears to function as a chaperone assisting in the folding of megalin in the endoplasmic reticulum and its transport to the cell surface. This review considers what is now known about the structure, function, and trafficking of megalin and RAP and the role of these two molecules in the pathogenesis of HN.


Subject(s)
Autoantigens/isolation & purification , Glomerulonephritis/immunology , Membrane Glycoproteins/immunology , Amino Acid Sequence , Animals , Antigen-Antibody Complex/biosynthesis , Autoantigens/genetics , Coated Pits, Cell-Membrane/immunology , Complement Activation , Disease Models, Animal , Endoplasmic Reticulum/immunology , Glomerulonephritis/etiology , Glomerulonephritis/genetics , Heymann Nephritis Antigenic Complex , Humans , Kidney Tubules, Proximal/immunology , Membrane Glycoproteins/genetics , Membrane Glycoproteins/physiology , Mice , Molecular Sequence Data , Rats , Receptors, LDL/genetics , Receptors, LDL/immunology , Receptors, LDL/physiology , Sequence Homology, Amino Acid
14.
J Am Soc Nephrol ; 6(1): 61-7, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7579071

ABSTRACT

The Heymann nephritis antigenic complex (HNAC) consists of two glycoproteins, megalin (gp330), and the receptor-associated protein (RAP). HNAC is expressed on the surface of the glomerular epithelium where it plays a primary role in the pathogenesis of Heymann nephritis (HN). Several models were previously proposed describing how antibody binding epitopes in HNAC may contribute to the initiation and progression of HN. Although these models suggest that nephritogenic epitopes capable of initiating HN are present in both megalin and RAP, the structural relationship between these epitopes has not been established. Previously a nephritogenic epitope was identified and characterized in RAP that initiates immune complex formation in HN. In this report, the immunologic relationship between nephritogenic epitopes in megalin and RAP were examined to determine whether these epitopes are immunologically distinct or antigenically related. To this end, a polyclonal antibody to megalin was generated that does not recognize RAP by immunoblotting or immunoprecipitation and whether this antibody is capable of inducing passive HN was determined. It was found that antimegalin antibodies devoid of RAP cross-reactivity induced the formation of subepithelial immune deposits (passive HN) when injected into rats. Antibodies eluted from glomeruli of the injected rats recognized only megalin by immunoblotting a cortical extract and did not recognize a RAP fusion protein or any other renal protein. In addition, the eluted antibodies immunoprecipitated two proteolytic fragments of megalin (140 and 75 kd) identifying a pathogenic epitope within a smaller fragment of megalin.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Autoantigens , Glomerulonephritis/etiology , Membrane Glycoproteins/immunology , Animals , Antibody Specificity , Cross Reactions , Disease Models, Animal , Epitopes , Glomerulonephritis/immunology , Glomerulonephritis/pathology , Heymann Nephritis Antigenic Complex , Male , Microscopy, Immunoelectron , Peptide Fragments/immunology , Rats , Rats, Inbred Lew
15.
J Cell Sci ; 108 ( Pt 4): 1433-41, 1995 Apr.
Article in English | MEDLINE | ID: mdl-7615664

ABSTRACT

The receptor-associated protein, RAP, is a chaperonin-like molecule that binds to two members of the low density lipoprotein receptor (LDLR) superfamily-megalin (gp330) and the LDL receptor-related protein (LRP). In F9 embryonal carcinoma cells, expression of RAP mRNA increases when differentiation is induced with retinoic acid and dibutyryl-cyclic AMP. We have investigated the expression of megalin and LRP and their interaction with RAP in F9 cells using biochemical and immunocytochemical methods. Both receptors are expressed in uninduced F9 cells, but only megalin co-precipitates with RAP. When F9 cells were induced to differentiate into parietal endoderm, the expression of megalin was dramatically increased. The expression of megalin exceeded that of LRP and RAP by an order of magnitude and both receptors co-precipitated with RAP. By immunoelectron microscopy, megalin and LRP were localized to clathrin-coated pits at the cell surface in both undifferentiated and differentiated F9 cells, whereas RAP was found mainly in the ER. A sizeable pool of LRP was also detected in the ER. When F9 cells were grown in suspension in the presence of RA and induced to develop into embryoid bodies, the expression of megalin and LRP segregated into different cell types: megalin was found in the outer epithelial layer and LRP in the stem cells of the inner core. Our results demonstrate that F9 cells induced to differentiate in monolayer culture express megalin, LRP and RAP, and RAP is capable of interacting simultaneously with both receptors. In embryoid bodies the expression of megalin and LRP diverges, and only megalin is expressed in the outer epithelial layer.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Cell Differentiation , Gene Expression , Membrane Glycoproteins/biosynthesis , Receptors, Immunologic/biosynthesis , Amino Acid Sequence , Animals , Antibodies , Bucladesine/pharmacology , Carcinoma, Embryonal , Cell Line , Gene Expression/drug effects , Heymann Nephritis Antigenic Complex , Immunoenzyme Techniques , Immunohistochemistry , Low Density Lipoprotein Receptor-Related Protein-1 , Membrane Glycoproteins/analysis , Membrane Glycoproteins/isolation & purification , Mice , Microscopy, Immunoelectron , Molecular Sequence Data , Peptides/chemistry , Peptides/immunology , RNA, Messenger/analysis , RNA, Messenger/biosynthesis , Receptors, Immunologic/analysis , Receptors, Immunologic/isolation & purification , Receptors, LDL/biosynthesis , Tumor Cells, Cultured
16.
Ann N Y Acad Sci ; 737: 96-113, 1994 Sep 10.
Article in English | MEDLINE | ID: mdl-7944157

ABSTRACT

It is apparent that significant progress has been made in the characterization of gp330 in the years that have elapsed since its initial identification as the nephritogenic antigen of Heymann nephritis. However, there are still many gaps in our knowledge and we do not yet have a full picture of the molecular events leading to the formation of immune deposits in glomerular capillaries. Moreover, we still do not have direct information on the normal function(s) of gp330 and RAP and their trafficking in renal and other epithelia. The availability of the yolk sac and other cell lines that express gp330 and RAP together with the identification of the functional domains of RAP should greatly facilitate experimental studies designed to elucidate these problems. Progress will also be greatly facilitated in the future when the complete amino-acid sequence of gp330 becomes available, making possible further structural studies. It is our hope that new knowledge obtained on the molecular mechanisms of HN will provide insights into the molecular pathogenesis of human membranous nephropathy and will provide a strategy for the design of appropriate treatments to interrupt the process.


Subject(s)
Autoantigens/immunology , Carrier Proteins/immunology , Glomerulonephritis/immunology , Glycoproteins/immunology , Kidney Glomerulus/immunology , Membrane Glycoproteins/immunology , Animals , Autoantigens/metabolism , Carrier Proteins/metabolism , Glycoproteins/metabolism , Heymann Nephritis Antigenic Complex , Humans , Kidney Glomerulus/pathology , LDL-Receptor Related Protein-Associated Protein , Membrane Glycoproteins/metabolism , Rats
17.
Proc Natl Acad Sci U S A ; 91(8): 3161-5, 1994 Apr 12.
Article in English | MEDLINE | ID: mdl-7512726

ABSTRACT

The receptor-associated protein (RAP) specifically associates with gp330 and the low density lipoprotein (LDL) receptor-related protein (LRP), the two newest members of the LDL receptor gene family. Results obtained by ligand blotting, affinity chromatography, and density-gradient sedimentation demonstrate that RAP binds to both receptors with high affinity and that the binding is Ca2+ dependent. RAP also binds heparin and is identical to a mouse heparin binding protein (HBP-44) identified in a teratocarcinoma cell line (F9). While biochemical studies have shown that RAP is present on the cell surface and is an effective inhibitor of ligand binding to gp330 and LRP, immunocytochemical findings indicate that RAP is most abundant in the endoplasmic reticulum lumen and may function in receptor folding and/or trafficking. To facilitate the characterization of RAP's function(s) we have mapped its gp330 and heparin binding sites by performing direct binding studies on fusion proteins representing overlapping domains of RAP. gp330 was found to bind to two separate sites on RAP--i.e., between amino acids 85-148 and 178-248. Binding studies with radiolabeled heparin indicate that the heparin binding site is between amino acids 261 and 323, which is consistent with our previously proposed site (residues 287-306) based on the amphipathic nature of the C terminus of RAP. These data demonstrate that the gp330 and heparin binding sites and the Heymann nephritis pathogenic epitope (amino acids 1-86) demonstrated earlier are represented by distinct domains of the RAP polypeptide.


Subject(s)
Carrier Proteins/chemistry , Glycoproteins/chemistry , Binding Sites , Carrier Proteins/immunology , Epitopes , Glomerulonephritis/immunology , Glycoproteins/immunology , Heparin/metabolism , Heymann Nephritis Antigenic Complex , In Vitro Techniques , LDL-Receptor Related Protein-Associated Protein , Membrane Glycoproteins/metabolism , Protein Binding , Recombinant Fusion Proteins , Structure-Activity Relationship
18.
Am J Pathol ; 143(5): 1423-35, 1993 Nov.
Article in English | MEDLINE | ID: mdl-8238258

ABSTRACT

Heymann nephritis in the rat is the most widely used model of human membranous glomerulonephritis. Glycoprotein (gp)330, a large (M(r) > 550,000) membrane-associated glycoprotein, has been identified as the main antigen in this autoimmune disease. Studies of gp330 and receptor-associated protein (RAP), its 44-kd subunit, have been restricted largely to rat kidney, as no stable cultured cell line has been available that expresses gp330. We have recently identified a rat yolk sac carcinoma cell line (L2) that expresses both gp330 and RAP. In this report, we have carried out detailed morphological, immunocytochemical, and biochemical studies characterizing the biosynthesis and localization of gp330 and RAP in the L2 rat yolk sac cell line. At the electron microscope level, the L2 cells are seen to be attached by cell junctions, and their predominant morphological features include extensive networks of rough endoplasmic reticulum (ER) and numerous clathrin-coated pits found on the cell membrane. By immunocytochemistry, gp330 was localized primarily to clathrin-coated pits at the cell surface, whereas RAP was localized predominantly to the lumen of the rough ER. Pulse-chase experiments indicated that gp330 spends a prolonged time maturing in the ER of L2 cells, as transport of gp330 to the Golgi complex (based on acquisition of endoglycosidase H resistance) is slow (t1/2 = 90 to 120 minutes). Gp330 reached the L2 cell surface beginning at 2 hours after synthesis, where it could be detected by cell surface immunoprecipitation. RAP was found to be an N-linked glycoprotein, and it remained endoglycosidase H-sensitive up to 4 hours after synthesis. Co-precipitation and co-sedimentation experiments demonstrated that gp330 and RAP form a large heterodimer (M(r) approximately 669,000) immediately after biosynthesis and are further assembled into a large hetero-oligomer in the ER. These findings demonstrate that the localization and the kinetics of assembly of gp330 and RAP into the Heymann nephritis antigenic complex are similar in both L2 cells and rat kidney. They also provide new information on the intracellular processing of these two molecules and their delivery to the cell surface. Thus, the L2 cell system should facilitate further characterization of the functions and interactions of gp330 and RAP, which may shed light on the cellular and molecular mechanisms of Heymann nephritis.


Subject(s)
Endodermal Sinus Tumor/chemistry , Endoplasmic Reticulum/chemistry , Membrane Glycoproteins/analysis , Ovarian Neoplasms/chemistry , Peptide Fragments/analysis , Animals , Biological Transport , Endodermal Sinus Tumor/ultrastructure , Female , Heymann Nephritis Antigenic Complex , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/metabolism , Microscopy, Electron , Ovarian Neoplasms/ultrastructure , Rats , Tumor Cells, Cultured
19.
Proc Natl Acad Sci U S A ; 90(9): 4082-6, 1993 May 01.
Article in English | MEDLINE | ID: mdl-8483924

ABSTRACT

gp330 is a large glycoprotein located in clathrin-coated pits at the surface of the glomerular and proximal tubule epithelia in the rat kidney. It was originally identified as the target of autoimmune antibodies in Heymann nephritis (HN) and has since been shown to be a member of the low density lipoprotein receptor gene family and to form a stable association with receptor-associated protein (RAP), which together constitute the HN antigen complex (HNAC). Progress in defining the normal functions of gp330 as well as the molecular mechanisms of HN has been hampered by the lack of an available kidney cell line that expresses this protein. We here report the identification of a rat yolk sac carcinoma cell line (L2) that synthesizes HNAC and expresses it in coated pits at the cell surface. gp330 and RAP from L2 cells are immunologically identical to their kidney counterparts, and peptide maps of gp330 yielded identical peptide fragments. Characterization of the cell line revealed that there are 3.3 x 10(4) gp330 molecules per L2 cell and that the cells produce a soluble form of gp330 that is released into the medium. Heparin ligand blot analysis demonstrated that RAP but not gp330 binds heparin. By heparin affinity chromatography, gp330 and RAP copurify, indicating that the glycosaminoglycan binding site within RAP is accessible when the subunit is complexed with gp330. These results indicate that the L2 cell line provides a valid and useful model for studies on the function of HNAC and the pathogenesis of HN.


Subject(s)
Autoantigens/metabolism , Coated Pits, Cell-Membrane/metabolism , Membrane Glycoproteins/metabolism , Receptors, LDL/metabolism , Animals , Autoantigens/isolation & purification , Cell Line , Cell Membrane/metabolism , Chromatography, Affinity , Cloning, Molecular , Endopeptidases , Glutathione Transferase/metabolism , Heparin/metabolism , Heymann Nephritis Antigenic Complex , Kidney/immunology , Membrane Glycoproteins/genetics , Membrane Glycoproteins/isolation & purification , Microvilli/immunology , Molecular Weight , Peptide Fragments/isolation & purification , Rats , Receptors, LDL/isolation & purification , Recombinant Fusion Proteins/metabolism
20.
Proc Natl Acad Sci U S A ; 89(23): 11179-83, 1992 Dec 01.
Article in English | MEDLINE | ID: mdl-1280820

ABSTRACT

Heymann nephritis is an experimental autoimmune disease model for human membranous nephropathy. We have recently identified a pathogenic epitope, clone 14 (C14), responsible for formation and deposition of glomerular immune complexes that is contained within the small subunit of the Heymann nephritis antigenic complex (HNAC). HNAC is a heterodimer composed of a large subunit designated gp330 and a smaller (44 kDa) subunit, which is immunologically identical to the receptor-associated protein. In this study, we prepared antibodies to fusion proteins with C-terminal deletions in the C14 sequence and assessed their ability to promote formation of immune deposits (IDs). When IgG specific for the shortest truncated fusion protein (C14/delta 3; 86 amino acids) was injected into rats, small IDs developed. In contrast, when IgG raised against the full-length C14 sequence was depleted of its reactivity toward the C14/delta 3 fusion protein (C14/delta 3-fp), no IDs could be detected. These data indicate that at least one pathogenic epitope is contained within the N-terminal 86 amino acids of C14. Since the IDs induced with the C14/delta 3-fp-specific IgG are smaller than those induced with the poly-epitope-specific anti-gp330 antibodies, it is likely that other epitopes in addition to those expressed by the C14/delta 3-fp are required for formation and growth of immune complexes.


Subject(s)
Glomerulonephritis/immunology , Membrane Glycoproteins/immunology , Animals , Autoantibodies/immunology , Blotting, Western , DNA Mutational Analysis , Epitopes , Heymann Nephritis Antigenic Complex , Immunization, Passive , Kidney Glomerulus/immunology , Membrane Glycoproteins/chemistry , Rats , Recombinant Proteins/immunology , Sequence Deletion
SELECTION OF CITATIONS
SEARCH DETAIL
...