ABSTRACT
Effects of a number of chemicals and of the time elapsed since the preparation on dentine permeability for microorganisms were under study. Dentine permeability was found the highest immediately after the preparation and after acid treatment. Potassium oxalate and calcium hydroxide reduced dentine permeability.
Subject(s)
Dental Caries/microbiology , Dentin/microbiology , Lactococcus lactis/pathogenicity , Animals , Dental Caries/therapy , Dental Pulp/microbiology , Dental Restoration, Permanent/methods , Dentin/drug effects , Dentin Permeability , Dentin-Bonding Agents/therapeutic use , Rabbits , Time FactorsSubject(s)
Clinical Clerkship/trends , Family Practice/education , Curriculum , Family Practice/trends , Humans , USSRABSTRACT
Some regularities of the enzyme immunoassay (EIA) of whole bacterial cells have been studied on one of the bacillary species of contaminant microflora. Early detection of this microorganism is highly important for the microbiological production of alpha-amylase and alkaline protease (produced by Bacillus subtilis). The effective kinetic and equilibrant parameters of the interaction of peroxidase-labeled antibodies with the cells of the contaminant microflora in the solution and on the surface of the polystyrene plates have been defined. Two methods for the separation of cells after their interaction with peroxidase-labeled antibodies have been optimized: filtration involving the use of special filter plates and centrifugation in plates. The method for the immobilization of cells in the wells of standard assay plates by centrifugation has been proposed. Four EIA methods for measurement of contaminant microflora have been developed and optimized. These methods permit the determination of the microflora at concentration of 5 X 10(5)-5 X 10(4) cells/ml, depending on the scheme of the assay, within 1-3.5 minutes.