Morphological variation and phylogeny of Karenia selliformis (Gymnodiniales, Dinophyceae) in an intensive cold-water algal bloom in eastern Hokkaido, Japan.

Harmful algal blooms responsible for mass mortalities of marine organisms have been rare in Hokkaido, northern Japan, although fish-killing blooms have been frequently reported from western Japanese coasts. In September-November 2021, a huge and prolonged cold-water bloom occurred along the Pacific coast of eastern Hokkaido, and was associated with intensive mortalities of sea urchin, fish, octopus, shellfish, etc. In this study, morphology and phylogeny of the dominant and co-occurring unarmored dinoflagellates of the Kareniaceae in the bloom were examined by using light microscopy, scanning electron microscopy and molecular phylogeny inferred from ITS and LSU rDNA (D1-D3) sequences. Morphological observation and molecular phylogeny showed that the dominant species was Karenia selliformis, with co-occurrences of other kareniacean dinoflagellates, Kr. longicanalis, Kr. mikimotoi, Karlodinium sp., Takayama cf. acrotrocha, Takayama tuberculata and Takayama sp. The typical cell forms of Kr. selliformis in the bloom were discoid, dorsoventrally flattened, and 35.3-43.6 (39.4  ±  2.1) µm in length, which was larger than the cell sizes in previous reports. Transparent cells of Kr. selliformis, lacking chloroplasts or having a few shrunken chloroplasts and oil droplets, were also found. Cells of Kr. selliformis showed morphological variation, but the species could be distinguished from other co-occurring Karenia species by the nucleus positioned in the hypocone and chloroplasts numerous (46-105) in number and small (2.9-4.6 µm) in diameter. Cell density of Kr. selliformis exceeding 100 cells mL-1 was recorded in the temperature range of 9.8-17.6 °C. The rDNA sequences determined from Kr. selliformis in the blooms of Hokkaido, Japan in 2021 were identical to those from the bloom in Kamchatka, Russia in 2020.

The harmful raphidophyte Chattonella (Raphidophyceae) in Western Pacific: Its red tides and associated fisheries damage over the past 50 years (1969-2019).

Red tides and associated fisheries damage caused by the harmful raphidophyte Chattonella were reassessed based on the documented local records for 50 years to understand the distribution and economic impacts of the harmful species in the Western Pacific. Blooms of Chattonella with fisheries damage have been recorded in East Asia since 1969, whereas they have been only recorded in Southeast Asia since the 1980s. Occurrences of Chattonella have been documented from six Southeast Asian countries, Indonesia, Malaysia, Philippines, Singapore, Thailand and Viet Nam, with mass mortalities mainly of farmed shrimp in 1980-1990s, and farmed fish in 2000-2010s. These occurrences have been reported with the names of C. antiqua, C. marina, C. ovata, C. subsalsa and Chattonella sp., owing to the difficulty of microscopic species identification, and many were not supported with molecular data. To determine the distribution of C. marina complex and C. subsalsa in Southeast Asia, molecular phylogeny and microscopic observation were also carried out for cultures obtained from Indonesia, Malaysia, Japan, Philippines, Russia, Singapore and Thailand. The results revealed that only the genotype of C. marina complex has been detected from East Asia (China, Japan, Korea and Russia), whereas both C. marina complex (Indonesia and Malaysia) and C. subsalsa (Philippines, Singapore and Thailand) were found in Southeast Asia. Ejection of mucocysts has been recognized as a diagnostic character of C. subsalsa, but it was also observed in our cultures of C. marina isolated from Indonesia, Malaysia, Japan, and Russia. Meanwhile, the co-occurrences of the two harmful Chattonella species in Southeast Asia, which are difficult to distinguish solely based on their morphology, suggest the importance of molecular identification of Chattonella genotypes for further understanding of their distribution and negative impacts.

Morphological and phylogenetic data do not support the split of Alexandrium into four genera.

A recently published study analyzed the phylogenetic relationship between the genera Centrodinium and Alexandrium, confirming an earlier publication showing the genus Alexandrium as paraphyletic. This most recent manuscript retained the genus Alexandrium, introduced a new genus Episemicolon, resurrected two genera, Gessnerium and Protogonyaulax, and stated that: "The polyphyly [sic] of Alexandrium is solved with the split into four genera". However, these reintroduced taxa were not based on monophyletic groups. Therefore this work, if accepted, would result in replacing a single paraphyletic taxon with several non-monophyletic ones. The morphological data presented for genus characterization also do not convincingly support taxa delimitations. The combination of weak molecular phylogenetics and the lack of diagnostic traits (i.e., autapomorphies) render the applicability of the concept of limited use. The proposal to split the genus Alexandrium on the basis of our current knowledge is rejected herein. The aim here is not to present an alternative analysis and revision, but to maintain Alexandrium. A better constructed and more phylogenetically accurate revision can and should wait until more complete evidence becomes available and there is a strong reason to revise the genus Alexandrium. The reasons are explained in detail by a review of the available molecular and morphological data for species of the genera Alexandrium and Centrodinium. In addition, cyst morphology and chemotaxonomy are discussed, and the need for integrative taxonomy is highlighted.

The tellurite-reducing bacterium Alteromonas macleodii from a culture of the toxic dinoflagellate Prorocentrum foraminosum.

The Alteromonas macleodii strain 2328 was isolated from a clonal culture of the toxic dinoflagellate Prorocentrum foraminosum. The strain exhibits a resistance to high K2TeO3 concentrations (2500 µg/mL). A study of the growth dynamics of the strain exposed to K2TeO3 has shown a longer lag phase and a reduced stationary phase compared to those during cultivation with no toxicant. The fatty acids profile is dominated by 16:1 (n-7), 16:0, 17:1, 15:0, 18:1 (n-7), and 17:0. The 2328 strain belongs to the Gammaproteobacteria and is related to the genus Alteromonas with 99-100% sequence similarity to some intra-genome allele variants (paralogues) of 16S rRNA from A. macleodii. A phylogenetic reconstruction (ML and NJ), based on HyHK amino acid sequences, has revealed that the analyzed 2328 strain forms a common cluster with A. macleodii strains. In the presented work, the ability of A. macleodii to reduce potassium tellurite to elemental tellurium has been recorded for the first time. Bacteria reduce potassium tellurite to Te (0), nanoparticles of which become distributed diffusely and in the form of electron-dense globules in cytoplasm. Large polymorphous metalloid crystals are formed in the extracellular space. Such feature of the A. macleodii strain 2328 makes it quite attractive for biotechnological application as an organism concentrating the rare metalloid.

Accumulation and Tissue Distribution of Dinophysitoxin-1 and Dinophysitoxin-3 in the Mussel Crenomytilus grayanus Feeding on the Benthic Dinoflagellate Prorocentrum foraminosum.

A DTX-1-producing microalga, Prorocentrum foraminosum, from Peter the Great Bay, Sea of Japan, was fed to Gray's mussels, Crenomytilus grayanus, for 12 days. An increase in DTX-1 and 7-O-acyl-DTX-1 (DTX-3) was observed in the digestive gland, kidneys, and gills. The digestive gland accumulated 91-100% of DTX-1 + DTX-3; and kidneys and gills accumulated, up to 8.5% and 4.3%, respectively. The kidneys had a distinctive pattern of toxin accumulation where the concentration of DTX-1 did not grow significantly after the eighth day of feeding, indicating the potential of DTX-1 elimination. The digestive gland and gills predominantly accumulated DTX-1, with a dramatic increase between Days 8 and 12. The DTX-3 content was highest in the digestive gland. The composition of DTX-3 in the acyl groups was similar for the digestive gland and kidneys, and did not change during feeding. The total toxin uptake of mussels exceeded the total toxin content from ingested cells by 2.4 times, showing that toxins may have accumulated from the seawater. This assumption needs to be further proved. The muscle, gonads, and mantle remained free of toxins. No genotoxic effect was observed in the gills and digestive gland.