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1.
RNA ; 17(12): 2094-107, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22028363

ABSTRACT

Aberrant mRNAs with premature translation termination codons (PTCs) are recognized and eliminated by the nonsense-mediated mRNA decay (NMD) pathway in eukaryotes. We employed a novel live-cell imaging approach to investigate the kinetics of mRNA synthesis and release at the transcription site of PTC-containing (PTC+) and PTC-free (PTC-) immunoglobulin-µ reporter genes. Fluorescence recovery after photobleaching (FRAP) and photoconversion analyses revealed that PTC+ transcripts are specifically retained at the transcription site. Remarkably, the retained PTC+ transcripts are mainly unspliced, and this RNA retention is dependent upon two important NMD factors, UPF1 and SMG6, since their depletion led to the release of the PTC+ transcripts. Finally, ChIP analysis showed a physical association of UPF1 and SMG6 with both the PTC+ and the PTC- reporter genes in vivo. Collectively, our data support a mechanism for regulation of PTC+ transcripts at the transcription site.


Subject(s)
Codon, Nonsense , RNA, Messenger/metabolism , Transcription, Genetic , Cell Line , Cytoplasm/genetics , Cytoplasm/metabolism , Gene Expression Regulation , Gene Order , Gene Silencing , Humans , Immunoglobulin mu-Chains/genetics , In Situ Hybridization, Fluorescence , Nonsense Mediated mRNA Decay , RNA Helicases , RNA Splicing , Telomerase/genetics , Telomerase/metabolism , Trans-Activators/genetics , Trans-Activators/metabolism
2.
PLoS Biol ; 6(4): e92, 2008 Apr 29.
Article in English | MEDLINE | ID: mdl-18447580

ABSTRACT

Translation termination at premature termination codons (PTCs) triggers degradation of the aberrant mRNA, but the mechanism by which a termination event is defined as premature is still unclear. Here we show that the physical distance between the termination codon and the poly(A)-binding protein PABPC1 is a crucial determinant for PTC recognition in human cells. "Normal" termination codons can trigger nonsense-mediated mRNA decay (NMD) when this distance is extended; and vice versa, NMD can be suppressed by folding the poly(A) tail into proximity of a PTC or by tethering of PABPC1 nearby a PTC, indicating an evolutionarily conserved function of PABPC1 in promoting correct translation termination and antagonizing activation of NMD. Most importantly, our results demonstrate that spatial rearrangements of the 3' untranslated region can modulate the NMD pathway and thereby provide a novel mechanism for posttranscriptional gene regulation.


Subject(s)
3' Untranslated Regions/metabolism , RNA Processing, Post-Transcriptional , Codon, Nonsense/metabolism , Humans , Poly A/metabolism , Poly(A)-Binding Protein I/metabolism , RNA Stability , RNA, Messenger/metabolism
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