Variables associated with corneal confocal microscopy parameters in healthy volunteers: implications for diabetic neuropathy screening.

AIM: Corneal confocal microscopy is a promising screening method for diabetic neuropathy. Although much research in this field has been accomplished, we aimed to determine and confirm the known clinical and eyewear variables associated with the parameters of corneal confocal microscopy specifically in healthy volunteers, in particular associations with corneal nerve fibre length. METHODS: Clinical characteristics, electrophysiological examination and a general clinical eye history were collected from 64 healthy volunteers. Corneal confocal microscopy was performed to determine corneal nerve fibre length, corneal nerve branch density, corneal nerve fibre density and tortuosity coefficient. Univariate and multivariate linear regression analysis was used to determine clinical variables associated with corneal nerve fibre length parameters. RESULTS: We observed that corneal nerve fibre length has a broad distribution in healthy volunteers (18 ± 4 mm/mm(2), 95% confidence interval, 12.3-25.7 mm/mm(2)). Multivariate regression analysis demonstrated that HbA(1c) was the only independent clinical factor to account for variations in corneal nerve fibre length, independent of age and status of contact lens wear. CONCLUSIONS: This study does not provide convincing evidence that corneal nerve fibre length is independently associated with age or the wearing of contact lenses, and that these factors are therefore unlikely to hinder valid screening for polyneuropathies such as diabetic neuropathy. Furthermore, the strong inverse association of corneal nerve fibre length with glycaemic exposure may support the use of this parameter to detect subclinical pre-diabetic nerve injury.

Reproducibility of in vivo corneal confocal microscopy as a novel screening test for early diabetic sensorimotor polyneuropathy.

AIM: With the goal of identifying a valid biomarker of early diabetic sensorimotor polyneuropathy, we aimed to identify the most reliable in vivo corneal confocal microscopy (CCM) parameter for detection of abnormality of small nerve fibre morphology. METHODS: Cross-sectional examination of 46 subjects (26 with Type 1 diabetes and 20 healthy volunteers) examined by corneal confocal microscopy for intra- and interobserver reproducibility by the intraclass correlation coefficient method. Corneal nerve fibre density, nerve branch density, nerve fibre length and tortuosity were measured on the same day that subjects underwent clinical and electrophysiological examination. RESULTS: The 26 subjects with Type 1 diabetes had mean age and diabetes duration 42.8 ± 16.9 and 22.7 ± 16.4 years, respectively. Twelve of those subjects (46%) did not meet criteria for diabetic sensorimotor polyneuropathy, while five (19%) had mild, three (12%) had moderate and six (23%) had severe diabetic sensorimotor polyneuropathy. None of the healthy volunteers (mean age 41.4 ± 17.3 years) had polyneuropathy. Re-examination of selected corneal confocal microscopy images or sets of 40 images yielded very good to excellent intraclass correlation coefficients for all parameters. However, only one parameter (corneal nerve fibre length) emerged with consistently very good reproducibility using a clinically relevant 'study-level' protocol of subject re-examination (intra-observer intraclass correlation coefficient 0.72; interobserver intraclass correlation coefficient 0.73). Despite no differences in intraclass correlation coefficient between subgroups, corneal nerve fibre length was significantly lower (14.76 vs. 16.15 mm/mm(2), P = 0.04) in those with diabetes. CONCLUSIONS: Development of corneal confocal microscopy may need to focus on the measurement of corneal nerve fibre length, as it appears to have superior reliability in comparison with other parameters, and as evidence exists for its potential as a clinical biomarker of early diabetic sensorimotor polyneuropathy.

Time-resolved Raman spectroscopy with subpicosecond resolution: vibrational cooling and delocalization of strain energy in photodissociated (carbonmonoxy)hemoglobin.

A Raman spectrometer that provides both subpicosecond resolution and independent, tunable pump and probe pulses is described. The spectrometer is employed to obtain time-resolved spectra of (carbonmonoxy)hemoglobin (HbCO) at times from 0.2 to 95 ps subsequent to ligand photodissociation. The spectra are interpreted in terms of a vibrationally hot heme that cools substantially in 10 ps. Concomitant with the proposed vibrational cooling is a slower relaxation, which we suggest results from a protein response to heme doming induced by ligand detachment. Results and interpretations are discussed in the context of current models of the heme photophysics and of hemoglobin reactivity.

Femtosecond spectroscopy of excitation energy transfer and initial charge separation in the reaction center of the photosynthetic bacterium Rhodopseudomonas viridis.

Reaction centers from the photosynthetic bacterium Rhodopseudomonas viridis have been excited within the near-infrared absorption bands of the dimeric primary donor (P), of the "accessory" bacteriochlorophylls (B), and of the bacteriopheophytins (H) by using laser pulses of 150-fsec duration. The transfer of excitation energy between H, B, and P occurs in slightly less than 100 fsec and leads to the ultrafast formation of an excited state of P. This state is characterized by a broad absorption spectrum and exhibits stimulated emission. It decays in 2.8 +/- 0.2 psec with the simultaneous oxidation of the primary donor and reduction of the bacteriopheophytin acceptor, which have been monitored at 545, 675, 815, 830, and 1310 nm. Although a transient bleaching relaxing in 400 +/- 100 fsec is specifically observed upon excitation and observation in the 830-nm absorption band, we have found no indication that an accessory bacteriochlorophyll is involved as a resolvable intermediary acceptor in the primary electron transfer process.

Continuous laser amplification in a monomode fiber longitudinally pumped by evanescent field coupling.

Amplification of continuous laser radiation propagating in a monomode optical fiber was obtained by evanescent field coupling within a pumped dye solution deposited in the neighborhood of the fiber core. A factor-of-25 signal gain was recorded for 632.8-nm He-Ne radiation and DCM dye solution.

Polarization effect in optical-fiber ring resonators.

The effect of birefringence on the resonance frequency of a closed-loop fiber-optic resonator is calculated.

Absolute distance measurements by CO(2) laser multiwavelength interferometry.

This paper describes a CO(2) laser interferometer allowing absolute distance measurements using a set of CO(2) lines. The method relies on the so-called fractional fringe technique. First, we introduce some theoretical topics related to this application of CO(2) spectroscopy. The second part of the paper is a description of the instrument.

Raman-lidar humidity sounding of the atmospheric boundary-layer.

A Raman-lidar designed for the measurement of water-vapor profiles in the atmospheric boundary-layer is presented. The light source is a frequency-doubled ruby laser; Raman returns are digitized and stored in a multichannel analyzer prior to computer processing. Profiles up to 1800 m with a vertical resolution of 30 m are obtained; the accuracy is about 15% at a height of 1000 m. The validity of the measurement is discussed, and lidar soundings are compared with coincident meteorological radio-soundings. As an application, successive lidar profiles are used to determine the height-time structure of water-vapor, illustrating the possibilities of the Raman-lidar.

[Laser emission]. / Etude de la largeur spectrale et des variations de longueur d'onde de la lumière emise parun laser déclenché.

In solid-state laser emission, the spectral width mainly depends on two factors: frequency shift and broadening due to the short duration of the spikes. The latter being more important as the emitted wave train is shorter and the envelope slope steeper, it is of interest to consider an O-switched laser. The importance of the frequency shift compared to spectral width is determined for single pulses by measuring variations of the emitted wavelength as a function of time. Experiments are carried out by using a high-speed rotating mirror camera. The time resolution is about 3.2 nsec. Some results are reported, deduced from streak photographs of Fabry-Pérot rings. For a particular mode, a wavelength shift of the order of 3 x 10(-3) A in 50 nsec was detected; this shift is not a linear function of time.