Aryl hydrocarbon receptor interacting protein (AIP) significantly influences prognosis of pancreatic carcinoma.

INTRODUCTION: Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor. Aryl hydrocarbon receptor interacting protein (AIP) in one of AHR ligands. The aim of this study is to analyze the prognostic influence of AIP in pancreatic carcinoma. MATERIAL AND METHODS: Retrospective case series with immunohistochemical analysis of AIP. We have estimated a multivariate Cox's model for the outcome (progression free and overall survival). RESULTS: 204 patients were included in the study. As expected prognosis was poor and 67.8% died of disease. As for AIP 9.8% of the cases showed nuclear staining of the epithelial tumor cells and 59.4% a cytoplasmic one. Stroma was stained in 53.1% of the cases. Univariate survival analysis revealed a significantly worse prognosis of patients with cytoplasmic AIP expression (stroma and epithelium), but nuclear expression was associated to a better prognosis. In the multivariate analysis stromal AIP expression was an independent prognosticator of progression free survival, together with pT stage, histological grade and history of diabetes. DISCUSSION: AIP Is a conserved cochaperone protein binding to many proteins. AIP has been proposed as a potential tumor suppressor gene. To date, no study has analyzed the immunohistochemical expression of AIP in pancreatic carcinoma. Our results indicate that both epithelial and stromal cytoplasmic expression of AIP is associated to bad prognosis, while nuclear translocation seems to improve prognosis. CONCLUSION: Although we must deepen into the complex signaling pathways underlying this potential association, our results open a way to inhibiting AHR as a potential target against pancreatic carcinoma.

Splenic volume as a biomarker of hepatic damage after chemotherapy in patients with resected colorectal liver metastases (CRLM).

BACKGROUND: Chemotherapy-associated liver injury (CALI) is a matter of concern for hepatobiliary surgeons as it can entail postoperative liver failure after an extensive hepatectomy. Recent studies have taken special interest in liver function parameters which can correlate with CALI to decrease this adverse event. Therefore, the current study investigates the usefulness of splenic volume as a biomarker of CALI through a portal hypertension mechanism, in patients with colorectal liver metastases (CRLM). STUDY DESIGN: We carried out a study in patients with CRLM operated on between 2009 and 2014 in our center. All samples of healthy liver were graded for non-alcoholic fatty liver disease (NAFLD) and sinusoidal obstructive syndrome. Computarized tomography scans for spleen volumetry were analyzed for each patient at CRLM diagnosis, after neoadjuvant chemotherapy, 1 and 6 months after resection. RESULTS: A group of 65 consecutive patients with CRLM of large bowel adenocarcinoma submitted to liver resection were included. Patients receiving neoadjuvant chemotherapy had a greater spleen volume increase than those who did not receive treatment (p = 0.053), finding a statistically significant spleen growth in patients with NAFLD (p = 0.036). There was no correlation between spleen enlargement and postoperative complications or average stay. However, survival was decreased in patients with spleen growth and CALI. CONCLUSIONS: Patients who receive neoadjuvant chemotherapy for liver metastasis surgery have a greater splenic volume increase, which correlates with NAFLD and a lower survival.

Still a place for conventional histopathological analysis in the era of molecular medicine: predicting prognosis of resectable ductal pancreatic adenocarcinoma.

INTRODUCTION: Our aim is to find features that define prognosis in surgically resected ductal pancreatic adenocarcinoma readily accessible in everyday practice. MATERIALS AND METHODS: Longitudinal retrospective case series of pancreatic adenocarcinoma operated with a curative intent in a large tertiary hospital in Madrid between 2009 and 2015. RESULTS: 162 were enrolled. 40.8% survived less than 1 year. Multivariate Cox's regression model revealed that gender, presence of symptoms, T and N stage independently influenced progression-free survival, while overall survival was determined by gender, smoking, presence of symptoms and N stage. Logistic regression analysis revealed that only symptoms at diagnosis could predict death, while gender, symptoms, histopathological type, vessel invasion, T stage and necrosis could independently predict recurrence. DISCUSSION: Our series show that patients with symptomatic disease at the time of diagnosis and females showed a shorter progression-free and overall survival. We herein propose a regression model to predict outcome.

Efecto del fresado y enclavado endomedular sobre la producción de factores de crecimiento en el callo de fractura de fémur en ratas / Effect of intramedullary reaming and nailing on the production of growth factors in the femur fracture callus in rats

Introducción. Existen muchos estudios referentes a los diferentes efectos producidos por el fresado intramedular en el callo de fractura, pero no existe evidencia en la literatura del efecto de dicho fresado en la osteogénesis. Realizamos un estudio prospectivo para analizar el efecto del fresado endomedular y enclavado en la producción de factores de crecimiento durante el proceso de consolidación de la fractura en el fémur producida en ratas. Material y métodos. Producimos una fractura diafisaria, transversa, no conminuta de fémur en 64 ratas: 34 ratas no recibieron ningún tratamiento y las otras 30 se trataron mediante un procedimiento quirúrgico estandarizado, consistente en fresado del canal medular de distal a proximal y fijación de la fractura con una aguja de Kirschner. Las ratas fueron sacrificadas a las 24 h, 4.°, 7.° y 15.° días después de la fractura. Medimos la cantidad de factores de crecimiento (PDGFA, TGF2 y TGFBeta-R2) en el callo de fractura mediante estudio anatomopatológico en los diferentes momentos del sacrificio. Resultados. Los resultados de las variables primarias del estudio, estratificadas por tiempo hasta el sacrificio, no mostraron diferencias estadísticas significativas. Discusión. Aunque la presencia de una aguja intramedular facilita la estabilización de la fractura y la formación del callo de fractura, no hemos encontrado ninguna evidencia significativa de que el fresado endomedular produzca cambios en la expresión de los factores de crecimiento estudiados (TGFBEta-R2, PDGFA y TGFBeta2) durante la formación del callo de fractura de fémur en ratas (AU)

Introduction: Many studies have been conducted to determine the different effects that reaming or intramedullary nailing have on fracture healing, but there is no evidence in the literature of the effect of intramedullary reaming on osteogenesis. We performed a prospective study to analyse the effect of intramedullary reaming and nailing on the production of growth factors during the process of fracture healing in the femur of rats. Material and methods: A transverse mid-shaft non-comminuted femur fracture was produced in 64 rats; 34 rats did not receive any treatment, and a standardized surgical procedure was performed on 30 rats, by exposing the left knee, reaming the medullary canal from distal to proximal, and then fixing the fracture with a steel pin. The rats were sacrificed at the 24th hour, 4th, 7th and 15th days after the fracture. The amount of growth factors that appeared in the callus fracture was measured using histopathology studies. The primary categorical variables analysed were PDGFA, TGF2 and TGFBeta-R2. These variables were analysed in each group at the different sacrifice times. Results: The results of the primary variables of the study, stratified by the time until sacrifice, showed no statistically significant differences. Discussion: Even if the presence of an intramedullary wire facilitates the fracture repair and the stabilising the bridge of bone between both edges of the fracture site, no evidence was found that reaming changes the expression of the growth factors studied (PDGFA, TGFBeta-R2 and TGFBeta) during the callus formation in rats (AU)

[Effect of intramedullary reaming and nailing on the production of growth factors in the femur fracture callus in rats]. / Efecto del fresado y enclavado endomedular sobre la producción de factores de crecimiento en el callo de fractura de fémur en ratas.

INTRODUCTION: Many studies have been conducted to determine the different effects that reaming or intramedullary nailing have on fracture healing, but there is no evidence in the literature of the effect of intramedullary reaming on osteogenesis. We performed a prospective study to analyse the effect of intramedullary reaming and nailing on the production of growth factors during the process of fracture healing in the femur of rats. MATERIAL AND METHODS: A transverse mid-shaft non-comminuted femur fracture was produced in 64 rats; 34 rats did not receive any treatment, and a standardized surgical procedure was performed on 30 rats, by exposing the left knee, reaming the medullary canal from distal to proximal, and then fixing the fracture with a steel pin. The rats were sacrificed at the 24th hour, 4th, 7th and 15th days after the fracture. The amount of growth factors that appeared in the callus fracture was measured using histopathology studies. The primary categorical variables analysed were PDGFA, TGF2 and TGFß-R2. These variables were analysed in each group at the different sacrifice times. RESULTS: The results of the primary variables of the study, stratified by the time until sacrifice, showed no statistically significant differences. DISCUSSION: Even if the presence of an intramedullary wire facilitates the fracture repair and the stabilising the bridge of bone between both edges of the fracture site, no evidence was found that reaming changes the expression of the growth factors studied (PDGFA, TGFß-R2 and TGFß2) during the callus formation in rats.

[Neurilemmoma of the larynx: a case report]. / Neurinoma laríngeo: a propósito de un caso.

Neuromas or Schwannomas are extremely rare among tumors of the larynx. They are Schwann cell tumors that can be difficult to distinguish from neurofibromas. They present usually as supraglottic masses, since they may arise from the internal branch of the superior laryngeal nerve. Nuclear magnetic resonance imaging is the best diagnostic technique, conferring a high degree of suspicion. We present an exceptional case of a laryngeal neuroma, with a very long evolution, a large tumor volume, dyspnea and vocal cord fixation, with complete resolution through an external approach following surgical removal. The difficulties encountered with its pathological and clinical diagnosis are discussed as well as a review of the literature.

Neurinoma laríngeo: a propósito de un caso / Neurilemmoma of the larynx: a case report

El neurinoma laríngeo es un tumor benigno extremadamente raro que deriva de las células de Schwann y se puede confundir con un neurofibroma. La forma de presentación más frecuente es una masa supraglótica que deriva de la rama interna del nervio laríngeo superior y es la RMN el método que mejor establece el diagnóstico de sospecha. Presentamos un caso tratado en nuestro servicio, cuya excepcionalidad radica en el largo tiempo de evolución, volumen elevado que conlleva disnea e inmovilidad de la hemilaringe afecta, que se recupera tras cirugía con abordaje externo. Se discuten las dificultades planteadas por su diagnóstico clínico y anátomo-patológico, al tiempo que se hace una revisión de la bibliografía pertinente (AU)

Neuromas or Schwannomas are extremely rare among tumors of the larynx. They are Schwann cell tumors that can be difficult to distinguish from neurofibromas. They present usually as supraglottic masses, since they may arise from the internal branch of the superior laryngeal nerve. Nuclear magnetic resonance imaging is the best diagnostic technique, conferring a high degree of suspicion. We present an exceptional case of a laryngeal neuroma, with a very long evolution, a large tumor volume, dyspnea and vocal cord fixation, with complete resolution through an external approach following surgical removal. The difficulties encountered with its pathological and clinical diagnosis are discussed as well as a review of the literature (AU)

Cambio conceptual de los tumores del estroma gastrointestinal (GISTs) y criterios de malignidad / No disponible

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[Spread channels of anterior commissure cancer: clinico-pathological study and surgical implications]. / Vías de diseminación del cáncer de comisura anterior: estudio clínico-patológico e implicaciones quirúrgicas.

UNLABELLED: The aim of the present study is to describe the spread channels of the anterior commisure cancer, its clinical significance and its surgical implications. PATIENTS AND METHODS: A prospective study was performed at the ENT Department of the Complutense University in about 31 patients with anterior commisure carcinoma wo underwent surgery between 1994/97. Specimens were processes with Whole-Organ Serial Sections. RESULTS: Patients were differenciated into two groups: 1) Patients with glottic tumors, with a good cord mobility and no invasive tumor in commisure region (18 patients); In these patients, conservative surgery was possible; 2) Another group (13 patients) with aggressive lesions (80% with cartilage involvement), vocal cord fixation and transglottic lesions. In these group, conservative surgery was contraindicated. CONCLUSIONS: This report emphasizes the importance of laryngoscopy in surgical technique of anterior commisure cancer.

Vías de diseminación del cáncer de comisura anterior: estudio clínico-patológico e implicaciones quirúrgicas / Spread channels of the anterior commisure cancer: a clinical and pathological study with surgical implications

El objetivo del estudio será la descripción de las vías de diseminación del cáncer de la comisura anterior y su implicación quirúrgica. Material y métodos: se realizó estudio histopatológico de 31 piezas de laringuectomía intervenidas entre 1994/97 mediante técnica de secciones seriadas de órgano completo. Resulta dos: los 31 pacientes con afectación de la región comisural anterior fueron divididos en dos grupos: 1) Un primer grupo de 18 pacientes en los que el tumor no invadía en profundidad la región comisural anterior que presentaban por laringoscopia tumores estrictamente del plano glótico con movilidad cordal conservada, en los que era posible realizar cirugía funcional; 2) Un segundo grupo de peor pronóstico, formado por 13 pacientes con invasión en profundidad de la región comisural anterior (83 por ciento con invasión del esqueleto cartilaginoso), todos ellos con invasión de más de un piso laríngeo y fijación cordal, en los que estaba contraindicada cualquier tipo de cirugía conservadora. Conclusiones: la imagen laringoscópica en el cáncer de la comisura anterior resulta fundamental para plantear cualquier intento de cirugía conservadora. (AU)

The aim of the present study is to describe the spread channels of the anterior commisure cancer, its clinical significance and its surgical implications. PATIENTS AND METHODS: A prospective study was performed at the ENT Department of the Complutense University in about 31 patients with anterior commisure carcinoma wo underwent surgery between 1994/97. Specimens were processes with Whole-Organ Serial Sections. RESULTS: Patients were differenciated into two groups: 1) Patients with glottic tumors, with a good cord mobility and no invasive tumor in commisure region (18 patients); In these patients, conservative surgery was possible; 2) Another group (13 patients) with aggressive lesions (80% with cartilage involvement), vocal cord fixation and transglottic lesions. In these group, conservative surgery was contraindicated. CONCLUSIONS: This report emphasizes the importance of laryngoscopy in surgical technique of anterior commisure cancer (AU)

Rotura capsular en el cáncer avanzado de laringe / Extracapsular spread in advanced laryngeal cancer

La rotura capsular del ganglio metastásico es un signo de muy mal pronóstico en los pacientes con cáncer de laringe. La identificación de esta rotura de la cápsula del ganglio por el tumor es difícil en el estudio prequirúrgico del paciente, incluso con las modernas técnicas de imagen. El objetivo de este trabajo será establecer parámetros histopatológicos predictivos de diseminación extracapsular en los ganglios metastásicos. Para ello hemos estudiado 11variables patológicas (tumorales -estadio T, localización tumoral, diámetro tumoral, invasión laríngea en profundidad, grado de diferenciación, invasión perineural, invasión cartilaginosa- y de las adenopatías cervicales- número de ganglios afectados, diámetro de los ganglios, bilateralidad de las metástasis cervicales, estadio N-) que podrían ser predictivas de rotura capsular en 47 pacientes con metástasis cervicales intervenidos quirúrgicamente en la Cátedra de ORL de la Universidad Complutense de Madrid entre los años 94/97. Todas las piezas quirúrgicas fueron estudiadas mediante Secciones Seriadas de Órgano Completo. Las variables que aumentaron el riesgo de rotura capsular en el presente estudio fueron la localización del tumor, el diámetro tumoral mayor de 2 cm, las presencia de 2-3 ganglios metastásicos y el estadio N (AU)

The aim of the present study is to evaluate histopathological features of extracapsular spread detected in patients with laryngeal cancer. PATIENTS AND METHODS: Specimens of 47 patients with laryngeal cancer were processed as Whole-Organ Serial Sections at the ENT Department of the Complutense University of Madrid between 1994/97. RESULTS: Extracapsular metastases were found in 21 patients. The risk for extracapsular spread was higher in those patients with: supraglottic or piriform sinus cancer, more than 2-3 neck metastatic nodes, tumor diameter of more than 2 cm and N2 stage (AU)

Cáncer glótico precoz: estudio clínicopatológico / Early glottic carcinoma: a clinical and pathological study

La displasia laríngea, el carcinoma in situ (Tis) y el carcinoma microinfiltrante (T1) de la cuerda vocal son parte del espectro histopatológico del cáncer glótico precoz. El objetivo del presente estudio es identificar la incidencia de malignización de las displasias de la cuerda vocal y las causas de recidiva tumoral del cáncer glótico precoz tratado con cirugía. Material y métodos: se realizó un estudio prospectivo de seguimiento desde el año 1992 sobre una cohorte de 134 pacientes con displasia-carcinoma in situ-cáncer glótico precoz de cuerda vocal. El grupo de estudio estaba formado por 68 displasias, 11 carcinomas in situ y 55 carcinomas microinfiltrantes. Resultados: Nueve pacientes de 68 displasias se malignizaron (13 per cent), mientras que seis pacientes con cáncer glótico precoz recidivaron localmente (11 per cent), todos ellos con afectación de la región comisural anterior. Se realizaron en total 8 laringectomías totales (6 per cent), falleciendo tres pacientes por enfermedad tumoral laríngea. Conclusión: El principal factor de riesgo de malignización del cáncer glótico precoz fue la afectación de la región comisural anterior (AU)

The aim of the present study is to evaluate histopathological features of malignization of laryngeal dysplasia and recurrence of early glottic carcinoma. PATIENTS AND METHODS: A prospective study was perform in a cohort of 134 patients who underwent surgery between 1992/99 at the ENT department of the Complutense University of Madrid. Tumor localization, malignization rate and recurrence rate were studied in 68 cases of laryngeal dysplasia, 11 of in situ-carcinoma and 55 of microinvasive carcinoma. RESULTS: Nine laryngeal dysplasias experienced invasive carcinoma (13%) and six patients with invasive glottic carcinoma had laryngeal recurrence (11%), all of them with anterior commisure involvement. Eight patients underwent total laryngectomy for recurrence (6%) and three patients died of laryngeal cancer. CONCLUSIONS: Anterior commisure involvement was the most important risk factor in early glottic carcinoma (AU)

Efecto protector de la vagotomía sobre la mucosa gástrica en un modelo de estrés en la rata / Protective effect of vagotomy on the gastric mucosa in a stress model in rats

Introducción: la utilización de la vagotomía se ha basado clásicamente en sus efectos inhibidores sobre la secreción ácida. La sección vagal provoca modificaciones celulares y endocrinas que podrían estar implicadas en sus acciones protectoras. Nuestro objetivo es estudiar los cambios morfológicos que provoca la vagotomía sobre la mucosa gástrica y su relación con la protección frente al estrés a corto, medio y largo plazo. Material y métodos: se ha empleado un modelo de estrés de inmovilización y frío y 80 ratas Wistar divididas en dos grupos, control (con y sin estrés) y vagotomía (a 7, 30 y 120 días). Se han estudiado los cambios provocados en la mucosa gástrica por el estrés, con y sin vagotomía, así como la relación entre estos cambios y la acción protectora frente al mismo. Resultados: el sangrado tuvo una relación muy significativa con el estrés (p< 0,0001). La incidencia de sangrado tuvo una diferencia significativa entre ratas vagotomizadas y no vagotomizadas (p< 0,0001) lo que ocurrió a corto, medio y largo plazo (la vagotomía fue protectora frente al estrés). Los signos de regeneración se relacionaban significativamente con la vagotomía (p< 0,0001) y no se relacionaban con el estrés (p= 0,208). Sin embargo, no había relación significativa entre la acción protectora y la presencia de signos de regeneración (p= 1). Conclusiones: la vagotomía tiene efectos tróficos sobre la mucosa gástrica y es protectora frente al estrés. Esta acción protectora se mantiene a corto, medio y largo plazo. Sin embargo, estos cambios no son suficientes para explicar la protección. En esta acción podría estar implicado un fenómeno de adaptación mediado por factores hormonales y peptídicos. (AU)

Hipercalcemia, nefrocalcinosis y tumoración de carpo bilateral como primeras manifestaciones de sarcoidosis en un niño de tres años / Hypercalcemia, nephrocalcinosis and bilateral carpal tumors as the first manifestations of sarcoidosis in a 3 3-year-old-boy

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Protective effect of vagotomy on the gastric mucosa in a stress model in rats.

INTRODUCTION: The use of vagotomy is classically based on its inhibiting effects on acid secretion. Vagotomy induces both cellular and endocrine changes that may be involved in protective actions. Our aim was to study morphologic changes induced by vagotomy on the gastric mucosa and their relation to stress protection in the short, medium and long term. MATERIAL AND METHODS: An immobilization and cold stress model was used with 80 Wistar rats divided into two groups--control (with and without stress) and vagotomy (at 7, 30 and 120 days). Changes induced in the gastric mucosa by stress were studied with and without vagotomy, as well as relationship between these changes and the intended protective action. RESULTS: Bleeding showed a very significant relation to stress (p < 0.0001). Bleeding incidence exhibited a significant difference between vagotomised and non-vagotomised rats (p < 0.0001) in the short, medium and long term (vagotomy was protective against stress). Regeneration signs related significantly to vagotomy (p < 0.0001) but not stress (p = 0.208). However, no significant relationship was found between the protective action and the presence of regeneration signs (p = 1). CONCLUSIONS: Vagotomy has tropic effects on the gastric mucosa and is protective against stress. This protective action is maintained in the short, medium and long term. However, these changes are not sufficient to explain protection. An adaptation phenomenon mediated by hormonal and peptidic factors may be involved in this action.

[Early glottic carcinoma: a clinico-pathological study]. / Cáncer glótico precoz: estudio clínico-patológico.

UNLABELLED: The aim of the present study is to evaluate histopathological features of malignization of laryngeal dysplasia and recurrence of early glottic carcinoma. PATIENTS AND METHODS: A prospective study was perform in a cohort of 134 patients who underwent surgery between 1992/99 at the ENT department of the Complutense University of Madrid. Tumor localization, malignization rate and recurrence rate were studied in 68 cases of laryngeal dysplasia, 11 of in situ-carcinoma and 55 of microinvasive carcinoma. RESULTS: Nine laryngeal dysplasias experienced invasive carcinoma (13%) and six patients with invasive glottic carcinoma had laryngeal recurrence (11%), all of them with anterior commisure involvement. Eight patients underwent total laryngectomy for recurrence (6%) and three patients died of laryngeal cancer. CONCLUSIONS: Anterior commisure involvement was the most important risk factor in early glottic carcinoma.

[Extracapsular spread in advanced laryngeal cancer]. / Rotura capsular en el cáncer avanzado de laringe.

UNLABELLED: The aim of the present study is to evaluate histopathological features of extracapsular spread detected in patients with laryngeal cancer. PATIENTS AND METHODS: Specimens of 47 patients with laryngeal cancer were processed as Whole-Organ Serial Sections at the ENT Department of the Complutense University of Madrid between 1994/97. RESULTS: Extracapsular metastases were found in 21 patients. The risk for extracapsular spread was higher in those patients with: supraglottic or piriform sinus cancer, more than 2-3 neck metastatic nodes, tumor diameter of more than 2 cm and N2 stage.

Carcinogénesis colónica en ratas vagotomizadas / Colonic carcinogenesis in vagotomyzed rats

Introducción: se ha comprobado un incremento de la incidencia de cáncer colorrectal en pacientes con enfermedad péptica tratados mediante vagotomía troncular y se ha considerado como un factor implicado en la etiología del cáncer colorrectal la inhibición ácida y su consecuencia hormonal, la hipergastrinemia. El objetivo de este estudio es ver si la vagotomía troncular incrementa, a corto (7 días) y a largo plazo (120 días), la incidencia de cáncer del colon, en un modelo de carcinogénesis. Material y método: utilizamos 86 ratas Wistar distribuidas en 7 grupos a las que administramos DMH (1,2-dimetilhidracina dihidroclorida), como inductor de tumoraciones en el colon, a dosis de 5 y 20 mg/kg de peso. A los tres primeros grupos les utilizamos como grupos control, y a las ratas de los cuatro últimos grupos las realizamos una vagotomía troncular con piloroplastia y miotomía de Heller, previa a la administración de DMH. Finalmente comparamos la incidencia de tumoraciones de colon entre los grupos no vagotomizados y los vagotomizados, a igual dosis de DMH administrada. Resultados: en las ratas no vagotomizadas y sometidas a dosis bajas de DMH (5 mg/kg de peso) la mortalidad fue del 0 por ciento, desarrollando cáncer el 0 por ciento frente al 40 por ciento y 0 por ciento respectivamente de las ratas vagotomizadas 7 días antes de administrar DMH y al 20 por ciento y 0 por ciento respectivamente de las ratas vagotomizadas 120 días antes de la administración de DMH. Tras la administración de dosis elevadas de DMH la mortalidad es del 50 por ciento y el desarrollo de cáncer del 80 por ciento frente al 100 por ciento y 0 por ciento respectivamente de las ratas vagotomizadas 7 días antes de la administración del DMH y al 61,11 por ciento y 42,8 por ciento respectivamente de las ratas vagotomizadas 120 días antes. Conclusión: la vagotomía troncular no aumenta la incidencia de cáncer de colon inducida por DMH en ratas (AU)

Colonic carcinogenesis in vagotomyzed rats.

INTRODUCTION: An increased incidence of colorectal cancer (CRC) has been reported in patients with peptic ulcer disease treated with truncal vagotomy. Inhibition of gastric acid output and its hormonal consequence, hypergastrinemia, have been considered risk factors for the development of CRC. The aim of the present study was to determine whether truncal vagotomy increases, in the short (7 days) and long term (120 days), the incidence of CRC in a model of carcinogenesis. MATERIAL AND METHOD: We used 86 Wistar rats distributed in 7 groups to which DMH (1,2-dimethylhydrazine dihydrochloride) was administered for the induction of colon tumors, at doses of 5 and 20 mg/kg of weight. The first three groups were used as control groups; the rats of the four other groups underwent a truncal vagotomy with pyloroplasty and Heller myotomy prior to the administration of DMH. Finally, we compared the incidence of colonic tumors in vagotomized vs non-vagotomized groups receiving the same dose of DMH. RESULTS: In the non-vagotomized rats that received low doses of DMH (5 mg/kg of weight), mortality was 0% and 0% developed cancer as compared to 40% and 0%, respectively, of rats vagotomized 7 days before the administration of DMH and 20% and 0%, respectively, of rats vagotomized 120 days before the administration of DMH. After the administration of high doses of DMH, mortality was 50% and 80% developed cancer as compared to 100% and 0%, respectively, of rats vagotomized 7 days before the administration of DMH and 61.11% and 42.8%, respectively, of rats vagotomized 120 days before the administration of DMH. CONCLUSION: Truncal vagotomy does not increase the incidence of CRC induced by DMH in the rat.