ABSTRACT
Introduction: DMBA is a chemical carcinogen that induces carcinomas within a few weeks of its application. We developed an experimental model of carcinogenesis induced by DMBA dissolved in 0,5% paraffin oil (DMBA-PO), verifying the inhibitory effect of the carcinogenicity of phenyl isothiocyanate (PhITC), phenethyl (PhnITC) and benzyl isothiocyanate (BITC). Material and Methods: For this, 88 hamsters were distributed into three groups: one exposed to DMBA-PO (Group 1, n=12), three subgroups (n=12) exposed to PhITC, PhnITC, BITC and DMBA-PO (Group 2, n=36) and four control subgroups (n=10) that were not exposed to the carcinogen in which PO (paraffin oil) and isothiocyanates were applied (Group 3, n=40). Results: The experiment had a duration of 20 weeks, at the end of which the inhibitory effect was established by comparing the lesions developed in the groups that received isothiocyanates with the group that was only treated with DMBA-PO. The carcinogenic effect of DMBA-PO is 100% (35 carcinomas) and the inhibitory effect was 0, whereas in the presence of isothiocyanates the carcinogenic effect decreases, with an inhibitory effect of 86% for BITC (5 carcinomas) and 74% for PhITC (9 carcinomas). Conclusion: The inhibitory effect for PhnITC is 80% in relation to invasive OSCC (1 carcinoma).
Introducción: El DMBA es un carcinógeno químico que induce carcinomas a las pocas semanas de su aplicación. Desarrollamos un modelo experimental de carcinogénesis inducida por DMBA disuelto en aceite de parafina al 0,5% (DMBA-Ap) comprobando el efecto inhibidor de la carcinogénesis de los isotiocianatos fenil (PhITC), fenetil (PhnITC) y bencil isotiocianato (BITC). Material y Métodos: Para ello, se distribuyeron 88 hámsteres en 3 grupos: uno expuesto al DMBA-Ap (Grupo 1, n=12), tres subgrupos (n=12) expuestos a PhITC, PhnITC, BITC y DMBA-Ap (Grupo 2, n=36) y cuatro subgrupos controles (n=10), no expuestos al carcinógeno en el que se aplicaron Ap e isotiocianatos (Grupo 3, n=40). Resultados:El experimento tuvo una duración de 20 semanas, al final de la cual se establece de forma comparativa el efecto inhibidor comparando las lesiones desarrolladas en los grupos que recibieron isotiocianatos con respecto al grupo tratado sólo con DMBA-Ap. El efecto carcinógeno del DMBA-Ap es del 100% (35 carcinomas) y el efecto inhibidor 0, mientras que en presencia de isotiocianatos el efecto carcinógeno disminuye, con un efecto inhibidor del 86% para BITC (5 carcinomas) y del 74% para el PhITC (9 carcinomas). Conclusión:El efecto inhibidor del PhnITC es del 80% en relación con el COCE invasivo (1 carcinoma).
Subject(s)
Animals , Male , Anticarcinogenic Agents/therapeutic use , 9,10-Dimethyl-1,2-benzanthracene/toxicity , Carcinogens , Isothiocyanates , Models, Animal , Carcinogenesis , Squamous Cell Carcinoma of Head and NeckABSTRACT
El DMBA es un carcinógeno químico que induce carcinomas a las pocas semanas de su aplicación. En 44 hámsteres machos distribuidos en 4 grupos DMBA disuelto en aceite de parafina (DMBA-Ap), DMBA disuelto en aceite de oliva (DMBA-Ao), aceite de parafina y aceite de oliva se aplicó diariamente, en las bolsas bucales, aceite de parafina o aceite de oliva virgen extra en las 2 primeras semanas, alternativamente, durante la aplicación bisemanal de DMBA al 0,5% disuelto en aceite de parafina o de oliva durante 5semanas, y diariamente, hasta la vigésima semana. Los animales de los grupos DMBA-Ao y aceite de oliva, recibieron una dieta adicional con aceitunas maduras variedad Picual. El efecto carcinógeno DMBA-Ap (35carcinomas) es el 100%, y el efecto inhibidor, 0. La combinación de aceite de oliva como disolvente del DMBA y la dieta ad líbitum con aceituna Picual tiene un efecto inhibidor del 80% solo 3 carcinomas intraepiteliales y 4 carcinomas verrugosos, y no se desarrollaron carcinomas invasores (AU)
DMBA is a carcinogen that induces carcinomas within a few weeks of application. Forty-four male hamsters were divided into four groups: DMBA dissolved in paraffin oil (DMBA-Po), DMBA dissolved in olive oil (DMBA-Oo), paraffin oil and olive oil. Their mouths were swabbed daily with paraffin oil or extra virgin olive oil alternatively for the first two weeks, during the biweekly application of DMBA at 0.5% diluted in paraffin oil or olive oil for five weeks and daily until the twentieth week. The animals in the DMBA-Oo and olive oil groups received an additional diet of mature Picual olives. The DMBA-Po carcinogen effect (35 carcinomas) is 100% and the inhibitory effect 0. The combination of olive oil as DMBA solvent and the ad libitum diet with Picual olive has an inhibitory effect of 80%, with only three intraepithelial carcinomas and four verrucous carcinomas occurring and no invasive carcinomas (AU)
