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1.
J Microsc ; 253(1): 54-64, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24251410

ABSTRACT

Inspired by a multiresolution community detection based network segmentation method, we suggest an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells in a first pilot investigation on two selected images. The image processing problem is framed as identifying segments with respective average FLTs against the background in FLIM images. The proposed method segments a FLIM image for a given resolution of the network defined using image pixels as the nodes and similarity between the FLTs of the pixels as the edges. In the resulting segmentation, low network resolution leads to larger segments, and high network resolution leads to smaller segments. Furthermore, using the proposed method, the mean-square error in estimating the FLT segments in a FLIM image was found to consistently decrease with increasing resolution of the corresponding network. The multiresolution community detection method appeared to perform better than a popular spectral clustering-based method in performing FLIM image segmentation. At high resolution, the spectral segmentation method introduced noisy segments in its output, and it was unable to achieve a consistent decrease in mean-square error with increasing resolution.


Subject(s)
Automation, Laboratory/methods , Cytological Techniques/methods , Image Processing, Computer-Assisted/methods , Microscopy, Fluorescence/methods
2.
Nucleic Acids Res ; 37(10): 3391-406, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19336418

ABSTRACT

The vertebrate kinetochore complex assembles at the centromere on alpha-satellite DNA. In humans, alpha-satellite DNA has a repeat length of 171 bp slightly longer than the DNA in the chromatosome containing the linker histone H1. The centromere-binding protein CENP-B binds specifically to alpha-satellite DNA with properties of a centromeric-linker histone. Here, we analysed if linker histone H1 is present at or excluded from centromeric chromatin by CENP-B. By immunostaining we detected the presence, but no enrichment or depletion of five different H1 subtypes at centromeric chromatin. The binding dynamics of H1 at centromeric sites were similar to that at other locations in the genome. These dynamics did not change in CENP-B depleted cells, suggesting that CENP-B and H1 co-exist in centromeric chromatin with no or little functional overlap. By bimolecular fluorescence complementation (BiFC) and Förster resonance energy transfer (FRET), we revealed that the linker histone H1 subtypes H1 degrees and H1.2 bind to centromeric chromatin in interphase nuclei in direct neighbourhood to inner kinetochore proteins.


Subject(s)
Autoantigens/metabolism , Centromere Protein B/metabolism , Centromere/metabolism , Chromosomal Proteins, Non-Histone/metabolism , Histones/metabolism , Centromere/chemistry , Centromere Protein A , Centromere Protein B/antagonists & inhibitors , Centromere Protein B/genetics , Chromatin/chemistry , Fluorescence Resonance Energy Transfer , HeLa Cells , Histones/analysis , Humans , Kinetochores/metabolism , Microscopy, Fluorescence , RNA Interference
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