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Butane and propane oxidation by engineered cytochrome P450cam.
Bell, Stephen G; Stevenson, Julie-Anne; Boyd, Helen D; Campbell, Sophie; Riddle, Austin D; Orton, Erica L; Wong, Luet-Lok.
Chem Commun (Camb) ; (5): 490-1, 2002 Mar 07.
Article in English | MEDLINE | ID: mdl-12120555

ABSTRACT

The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidizes butane with a turnover rate of 750 min-1 and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min-1 with 4% yield.


Subject(s)
Butanes/metabolism , Camphor 5-Monooxygenase/metabolism , Propane/metabolism , Binding Sites/genetics , Butanols , Camphor 5-Monooxygenase/genetics , Kinetics , Mutagenesis, Site-Directed , Oxidation-Reduction , Propanols , Protein Engineering , Pseudomonas putida/enzymology
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