ABSTRACT
Staple-line bleeding and leakage is a life-threatening complication in obese patients following laparoscopic sleeve gastrectomy. The aim of this study was to examine the potential effects of Ankaferd blood stopper (ABS) and Fibrin Sealant (FS; Tisseel®) on sleeve gastrectomy staple-line healing in an experimental animal model. A total of 30 Wistar albino female rats were divided into three groups and were subjected to sleeve gastrectomy with linear stapling. Group A (control group) had nothing administered, Group B was administered FS on the staple-line, and Group C was administered ABS on the staple-line following sleeve gastrectomy. After sacrifice on postoperative day 5, anastomotic burst pressure, tissue hydroxyproline levels and histopathological parameters were measured. The results revealed that group C had the highest mean bursting pressure level. However, the values of this parameter were not found to differ significantly between the groups (P>0.05). Group B and C had a similar hydroxyproline levels but increased compared with group A (P<0.001). Histopathological parameters were similar between the groups, except macrophage scores in group C. In the present experimental study, ABS was demonstrated to improve gastric-sleeved staple-line healing compared with FS. ABS may be used as a novel reinforcement agent in bariatric surgery.
ABSTRACT
OBJECTIVE: Cystic echinococcosis (CE) is caused by metacestodes of Echinococcus granulosus, which is one of the most widespread zoonotic diseases in humans in both developing and developed countries, and also in Turkey. The aim of this retrospective study was to evaluate the situation of hydatid disease in Kocaeli. METHODS: The specific anti-Echinococcus granulosus indirect haemagglutination test results of 225 patients, who were referred with probable CE to the Centre Laboratory of the Kocaeli Derince Education and Research Hospital during December 2009-May 2011 was assessed retrospectively. Positive cases were also reassessed clinically. RESULTS: Of the total, 151 (67.1%) were female and 74 (32.8%) were male. The seropositivity ratio of IHA test was found to be 8% (18 patients), borderline ratio as 2.2% (5 patients), and seronegative ratio as 89.8% (202 patients). In 15 of the 23 seropositive and borderline patients, CE compatible radiological lesions were determined, while 4 of the remaining patients showed no lesion and the other 4 had no radiological data. CONCLUSION: Considering that hospital records can represent only a small part of the CE cases, it can be said that CE still subsists and retains its importance in our city. Essential precautions should be taken for the prevention and protection for this disease.
Subject(s)
Echinococcosis/epidemiology , Echinococcus granulosus/immunology , Adolescent , Adult , Aged , Aged, 80 and over , Animals , Antibodies, Helminth/blood , Child , Child, Preschool , Female , Hemagglutination Tests/methods , Humans , Male , Middle Aged , Probability , Retrospective Studies , Turkey/epidemiology , Young Adult , Zoonoses/epidemiologyABSTRACT
BACKGROUND/AIMS: Although studies have reported that xanthine oxidase inhibitors or calcium channel blockers attenuate the ischemia-reperfusion injury in several organ systems, no comparative study exists on the significance of each of these pathways. To study this, in anesthetized Wistar Albino rats, a surgical model for intestinal ischemia-reperfusion injury was employed. METHODOLOGY: In experimental animals, after laparotomy, the superior mesenteric artery was occluded for 30 min, followed by a 2-h period of reperfusion; control rats underwent only a sham laparotomy procedure. One group of experimental animals was pretreated intraperitoneally with the calcium channel blocker verapamil (0.3 mg/kg), another group with the xanthine oxidase inhibitor allopurinol (100 mg/kg), the third group received no pretreatment. Plasma lactate, malondialdehyde and glutathione levels as well as intestinal tissue malondialdehyde and glutathione levels were measured to assess for possible protective effects. Histologic evaluation of the extent of injury was also performed. RESULTS: Irreversible tissue damage was depicted in the untreated group, and partially in the allopurinol pretreatment group by histologic examination. Ischemia-reperfusion injury was reversible in the verapamil group. The laboratory results also supported these findings. CONCLUSIONS: Protective effects of verapamil on ischemia-reperfusion injury have been found to be significantly (p<0.0001) more effective compared to allopurinol.
