ABSTRACT
The blocking of polyamine synthesis in cancer cell by a specific inhibitor (DFMO) results in an increase in membrane viscosity and in significant changes in cell deformability as determined by cell penetration through a nuclear filter with given porosity. Almost two-fold reduction of the penetration rate through the filter pores was registered for the DFMO-treated cells in spite of the fact that the cell size decreases after such a treatment. The number of cells penetrating through the filter after DFMO treatment is diminished due to an increasing resistance to penetration. The addition of putrescine to the incubation medium at physiological concentration restores all these parameters to the initial values. The possible association of these changes with differential sensitivity of cancer cells towards the inhibitor action depending on the stage of cell cycles is discussed.
Subject(s)
Carcinoma, Ehrlich Tumor/metabolism , Eflornithine/toxicity , Polyamines/antagonists & inhibitors , Animals , Carcinoma, Ehrlich Tumor/chemically induced , Carcinoma, Ehrlich Tumor/pathology , Cell Membrane/metabolism , Cell Membrane/pathology , Cell Membrane Permeability , MiceABSTRACT
A decrease of electrophoretic mobility of the blood red cell under the influence of the microwave and thermal field is determined by the mechanism based on two effects--change of the ionic conductivity and visco-elastic properties of the membrane.
Subject(s)
Erythrocytes/cytology , Membrane Fluidity/radiation effects , Radio Waves , Cell Movement/radiation effects , Chemical Phenomena , Chemistry, Physical , Electrophoresis , Erythrocyte Membrane/radiation effects , Humans , Models, BiologicalABSTRACT
A decrease of electrophoretic mobility is revealed in white rat erythrocytes under the effect of microwave radiation. This decrease depends on duration of irradiation. The mechanism of the influence of microwave radiation on the erythrocytes is suggested.
