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1.
Int J Immunopathol Pharmacol ; 21(2): 279-88, 2008.
Article in English | MEDLINE | ID: mdl-18547470

ABSTRACT

Epidemiological and experimental studies have suggested that diesel exhaust particles (DEPs), which generate reactive oxygen species, may be involved in the recent increase in the prevalence of lung diseases. Cacao liquor proanthocyanidins (CPs) are naturally occurring polyphenols with antioxidative activities. We carried out a study in mice to investigate the effects of dietary supplementation of CPs on lung injury induced by intratracheal administration of DEPs (500 microg/body). Dietary supplementation with 1.0 percent CPs inhibited DEP-induced lung injury, characterized by neutrophil sequestration and edema. Immunohistochemical analyses showed that CPs prevented enhanced expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 caused by DEPs in the lung injury. Numerous adducts of nitrotyrosine, N-(hexanonyl) lysine, 4-hydroxy-2-nonenal, and 8-OHdG were also observed immunohistochemically in the lungs of mice treated with DEPs. However, these indicators of oxidative stress were barely visible in mice pretreated with CP supplementation. In addition, the level of thiobarbituric acid reactive substances in the lung was decreased by CP supplementation in the presence of DEPs. These results suggest that CPs inhibit DEP-induced lung injury by reducing oxidative stress, in association with a reduction in the expression of adhesion molecules.


Subject(s)
Cacao/chemistry , Lung Diseases/prevention & control , Proanthocyanidins/pharmacology , Vehicle Emissions/toxicity , Animals , Bronchoalveolar Lavage Fluid/cytology , Catechin/chemistry , Catechin/pharmacology , Cell Adhesion Molecules , Chemokines/biosynthesis , Cytokines/biosynthesis , Immunohistochemistry , Indicators and Reagents , Intubation, Intratracheal , Lipid Peroxidation/drug effects , Lung Diseases/chemically induced , Male , Mice , Mice, Inbred ICR , Oxidative Stress/drug effects , Proanthocyanidins/chemistry , Thiobarbituric Acid Reactive Substances/metabolism
2.
Neuroscience ; 138(4): 1205-13, 2006.
Article in English | MEDLINE | ID: mdl-16460878

ABSTRACT

Densely aggregated beta-amyloid peptides are believed to play a key role in the pathogenesis of Alzheimer's disease. Amyloid plaques are a potential target for molecular imaging to determine the clinical status of Alzheimer's disease. Phase-contrast X-ray imaging combined with computed tomography is a promising technique that can be used to visualize the physical density of structures in biological tissues non-invasively, and without the use of imaging agents. Using brain tissue isolated from a mouse model of Alzheimer's disease, we show that beta-amyloid 40-positive/beta-amyloid 42-positive amyloid plaques, but not beta-amyloid 40-negative/beta-amyloid 42-positive amyloid plaques, exist as high-density aggregates that can be specifically detected by phase-contrast X-ray computed tomography. The phase-contrast X-ray computed tomography detected beta-amyloid 40-positive/beta-amyloid 42-positive amyloid plaques in three-dimensions with an extremely high sensitivity comparable to that of histological analysis, and also enabled the load of amyloid plaques to be quantified. Furthermore, the use of phase-contrast X-ray computed tomography reveals that the physical density of beta-amyloid 40-positive/beta-amyloid 42-positive amyloid plaques increases with age, and that the large volume, high-density, amyloid plaques that are specifically observed in aged Alzheimer's disease mice are closely associated with neuritic dystrophy. These results demonstrate that phase-contrast X-ray computed tomography is a highly sensitive imaging technique for analyzing dense-cored amyloid plaques in postmortem samples, and is beneficial in elucidating amyloid pathophysiology in Alzheimer's disease.


Subject(s)
Alzheimer Disease/diagnostic imaging , Cerebral Cortex/diagnostic imaging , Cerebral Cortex/pathology , Plaque, Amyloid/diagnostic imaging , Plaque, Amyloid/pathology , Tomography, X-Ray Computed/methods , Aging/pathology , Alzheimer Disease/physiopathology , Amyloid beta-Peptides/metabolism , Animals , Cerebral Cortex/metabolism , Disease Models, Animal , Female , Mice , Mice, Transgenic , Microscopy, Phase-Contrast/methods , Neurites/metabolism , Neurites/pathology , Peptide Fragments/metabolism , Plaque, Amyloid/metabolism , Predictive Value of Tests
3.
Clin Exp Allergy ; 34(6): 971-7, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15196288

ABSTRACT

BACKGROUND: Perilla and its constituent rosmarinic acid have been suggested to have anti-allergic activity. However, few studies have examined the effects on allergic asthma. OBJECTIVE: The purpose of this study was to evaluate the effect of oral administration of perilla leaf extract, which contains high amount of rosmarinic acid, on a murine model of allergic asthma induced by house dust mite allergen. METHODS: C3H/He mice were sensitized by intratracheal administration of Dermatophagoides farinae (Der f). Mice were orally treated with rosmarinic acid in perilla extract (PE) (1.5 mg/mouse/day). RESULTS: Der f challenge of sensitized mice elicited pulmonary eosinophilic inflammation, accompanied by an increase in lung expression of IL-4 and IL-5, and eotaxin. Daily treatment with rosmarinic acid in PE significantly prevented the increases in the numbers of eosinophils in bronchoalveolar lavage fluids and also in those around murine airways. Rosmarinic acid in PE treatment also inhibited the enhanced protein expression of IL-4 and IL-5, and eotaxin in the lungs of sensitized mice. Der f challenge also enhanced allergen-specific IgG1, which were also inhibited by rosmarinic acid in PE. CONCLUSION: These results suggest that oral administration of perilla-derived rosmarinic acid is an effective intervention for allergic asthma, possibly through the amelioration of increases in cytokines, chemokines, and allergen-specific antibody.


Subject(s)
Cinnamates/administration & dosage , Hypersensitivity/drug therapy , Perilla frutescens , Phytotherapy , Plant Extracts/administration & dosage , Administration, Oral , Allergens , Animals , Depsides , Hypersensitivity/immunology , Male , Mice , Mice, Inbred C3H , Mites , Rosmarinic Acid
4.
J Agric Food Chem ; 49(12): 6050-6, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11743807

ABSTRACT

(-)-Epicatechin is a major polyphenol component of cocoa powder. The absorption and urinary excretion of (-)-epicatechin following administration of different levels of either cocoa powder (150, 750, and 1500 mg/kg) or (-)-epicatechin (1, 5, and 10 mg/kg) were evaluated in rats. Both the sum of plasma (-)-epicatechin metabolites at 1 h postadministration and peak plasma concentrations increased in a dose-dependent fashion. The sum of (-)-epicatechin metabolites in urine, excreted within 18 h postadministration, also increased with dose. Moreover, the sum of (-)-epicatechin metabolites excreted in urine reached the same level in both (-)-epicatechin and cocoa powder administration groups for equivalent amounts of (-)-epicatechin. These results suggest that, in the dose range examined in this study, bioavailability of (-)-epicatechin following administration of either (-)-epicatechin or cocoa powder shows dose dependence and that the various compounds present in cocoa powder have little effect on the bioavailability of (-)-epicatechin in cocoa powder.


Subject(s)
Cacao/chemistry , Catechin/metabolism , Animals , Catechin/analogs & derivatives , Catechin/urine , Chromatography, High Pressure Liquid , Intestinal Absorption , Lipid Peroxides/blood , Male , Mass Spectrometry , Oxidation-Reduction , Rats , Rats, Sprague-Dawley
5.
Food Chem Toxicol ; 39(12): 1279-83, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11696402

ABSTRACT

Oxidative DNA damage has been implicated as a factor playing a role in mutagenesis and carcinogenesis. We investigated the anticlastogenic activity of cacao: the inhibitory effect of cacao liquor polyphenols on DNA strand cleavage induced by mitomycin C (MMC) in vitro and the anticlastogenic effect of cacao liquor extract against formation of micronuclei induced by MMC in bone marrow cells and peripheral blood cells of mice. In the DNA strand cleavage test, cacao liquor polyphenols inhibited cleavage of RFI DNA. In the micronuclei test, the frequency of occurrence of micronucleated cells among bone marrow cells and peripheral blood cells were reduced significantly when cacao liquor extract was administered orally to mice 6 h before intraperitoneal injection of MMC. These findings suggest that cacao liquor polyphenols are effective in preventing DNA damage, and one of the mechanisms of action might involve scavenging of active oxygen radicals generated in reactions initiated by MMC.


Subject(s)
Antimutagenic Agents/pharmacology , Cacao/chemistry , DNA Damage/drug effects , Flavonoids , Mitomycin/antagonists & inhibitors , Phenols/pharmacology , Polymers/pharmacology , Animals , Bone Marrow/drug effects , Mice , Micronuclei, Chromosome-Defective , Micronucleus Tests , Mitomycin/toxicity , Mutagens/toxicity , Mutation/drug effects , Plant Extracts/chemistry , Plant Extracts/pharmacology , Polyphenols
6.
J Nutr ; 131(11): 2885-91, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11694613

ABSTRACT

We compared levels of (+)-catechin, (-)-epicatechin, and their metabolites in rat plasma and urine after oral administration. Rats were divided into four groups and given (+)-catechin (CA group), (-)-epicatechin (EC group), a mixture of the two (MIX group) or deionized water. Blood samples were collected before administration and at designated time intervals thereafter. Urine samples were collected 0-24 h postadministration. (+)-Catechin, (-)-epicatechin and their metabolites in plasma and urine were analyzed by HPLC-mass spectrometry after treatment with beta-glucuronidase and/or sulfatase. After administration, absorbed (+)-catechin and (-)-epicatechin were mainly present in plasma as metabolites, such as nonmethylated or 3'-O-methylated conjugates. In the CA and MIX groups, the primary metabolite of (+)-catechin in plasma was glucuronide in the nonmethylated form. In the EC and MIX groups, in contrast, the primary metabolites of (-)-epicatechin in plasma were glucuronide and sulfoglucuronide in nonmethylated forms, and sulfate in the 3'-O-methylated forms. Urinary excretion of the total amount of (-)-epicatechin metabolites in the EC group was significantly higher than the amount of (+)-catechin metabolites in the CA group. The sum of (+)-catechin metabolites in the urine was significantly lower in the MIX group than in the CA group, and the sum of (-)-epicatechin metabolites in the MIX group was also significantly lower than in the EC group. These results suggest that the bioavailability of (-)-epicatechin is higher than that of (+)-catechin in rats, and that, in combination, (+)-catechin and (-)-epicatechin might be absorbed competitively in the gastrointestinal tract of rats.


Subject(s)
Catechin/pharmacokinetics , Administration, Oral , Animals , Area Under Curve , Biological Availability , Catechin/metabolism , Catechin/urine , Chromatography, High Pressure Liquid , Chromatography, Liquid , Intestinal Absorption , Male , Methylation , Rats , Rats, Sprague-Dawley , Stereoisomerism
7.
Free Radic Res ; 34(1): 93-9, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11235000

ABSTRACT

Nine male volunteers were given 36 g of cocoa powder (containing 2610 mg of polyphenols) per day with sugar and 6 volunteers received an equivalent amount of sugar for 2 weeks. Conjugated diene production in LDL induced by 2-2' azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) and copper ion were evaluated. The lag time was significantly prolonged at 1 and 2 weeks in V-70 and at 2 weeks in copper ion after cocoa powder consumption. The level of excretion of epicatechin in urine was significantly higher in the cocoa group than that in the control group. In conclusion, the antioxidants in cocoa powder might be absorbed and increase the resistance of human LDL to oxidation.


Subject(s)
Antioxidants/metabolism , Cacao , Flavonoids , Lipoproteins, LDL/metabolism , Adult , Catechin/blood , Catechin/metabolism , Catechin/urine , Energy Intake , Humans , Lipoproteins, LDL/drug effects , Male , Oxidation-Reduction , Phenols/blood , Phenols/urine , Polymers
8.
J Agric Food Chem ; 48(10): 5074-8, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11052780

ABSTRACT

We investigated the effect of polyphenols derived from cacao liquor on the mutagenic action of heterocyclic amines (HCAs) in vitro and ex vivo. In the Ames test, the cacao liquor polyphenols showed antimutagenic effects in bacteria treated with HCA in the presence of an S-9 mixture; however, they showed less efficacy than quercetin. On the other hand, the cacao liquor polyphenols showed potent antimutagenic activity in bacteria treated with activated forms of HCA, compared with quercetin. We also evaluated the effect of these compounds on enzymatic activation of HCA. They weakly suppressed the production of activated HCA. In the host-mediated assay in mice, a method used to estimate the potential carcinogenicity of chemicals ex vivo, oral administration of the cacao liquor polyphenols, reduced the number of colonies of revertant bacteria recovered from the liver. These data suggest that the cacao liquor polyphenols have an antimutagenic effect not only in vitro, but also ex vivo.


Subject(s)
Antimutagenic Agents/chemistry , Antimutagenic Agents/pharmacology , Cacao/chemistry , Flavonoids , Heterocyclic Compounds/antagonists & inhibitors , Heterocyclic Compounds/toxicity , Mutagens/toxicity , Phenols/chemistry , Phenols/pharmacology , Polymers/chemistry , Polymers/pharmacology , Animals , In Vitro Techniques , Mutagenicity Tests , Polyphenols , Rats
9.
Br J Nutr ; 84(5): 673-80, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11177180

ABSTRACT

The aims of the present study were to determine the level of (-)-epicatechin (EC) and its metabolites in rat plasma after oral administration of cocoa powder and to evaluate the protective effect of cocoa powder in terms of suppressing the oxidation of plasma components. Rats were orally administered 1 g cocoa powder/kg body weight, containing 7.80 mg EC, and their blood was collected before administration and at designated time intervals thereafter. The EC and its metabolites in plasma were treated with beta-glucuronidase and/or sulfatase, then analysed by HPLC and by liquid chromatography-MS. Several EC-related compounds were detected in plasma such as free EC, and glucuronide, sulfate, and glucuronide-sulfate conjugates of non-methylated or methylated EC. All EC metabolites showed a maximum concentration in plasma at 30-60 min post-administration. Glucuronide conjugates of both non-methylated and methylated EC were found in high concentration in plasma. Moreover, administration of cocoa powder significantly reduced the accumulation of lipid peroxides in plasma and significantly reduced the consumption of alpha-tocopherol in plasma oxidized by treatment with 2,2'-azobis-(2-amidinopropane) dihydrochloride (AAPH (25 mmol/l)) or CuSO(4) (100 micromol/l) compared with that in the case of plasma obtained before administration. The total EC concentration in plasma was negatively correlated with the level of accumulation of lipid peroxides in plasma oxidized by treatment with AAPH (25 mmol/l) and was positively correlated with the level of residual alpha-tocopherol in plasma oxidized by treatment with CuSO(4) (100 micromol/l). These results indicate that EC in cocoa powder was absorbed from the digestive tract, that various conjugated forms of EC were generated in the digestive tract and distributed to the plasma, and that these enhanced the antioxidative activity of plasma.


Subject(s)
Cacao , Catechin/blood , Administration, Oral , Animals , Catechin/metabolism , Chromatography, High Pressure Liquid , Lipid Peroxides/analysis , Male , Rats , Rats, Sprague-Dawley , Vitamin E/analysis , Vitamin E/metabolism
10.
J Nutr Sci Vitaminol (Tokyo) ; 46(4): 199-204, 2000 Aug.
Article in English | MEDLINE | ID: mdl-11185658

ABSTRACT

Cacao is rich in polyphenols such as (-)-epicatechin, and a colored component of cacao (cacao-red) is polyphenol, which is an antioxidant. These properties stimulated an investigation of the effects of cacao liquor polyphenols (CLP) on low-density lipoprotein (LDL) oxidation. The 2.2 '-azobis(4-methoxy-2,4-dimethylvaleronitrile) (AMVN-CH2O)-induced oxidizability of LDL was assessed by monitoring the absorbance at 234 nm. In vitro. 0.1-0.5 mg/dL CLP prolonged the oxidation lag time of LDL in a dose-dependent manner. Compared with the controls, it was prolonged 1.7-fold in the presence of 0.1 mg/dL CLP, 2.9-fold at 0.2 mg/dL, 3.8-fold at 0.3 mg/dL, 5.4-fold at 0.4 mg/dL, and 6.4-fold at 0.5 mg/dL. Furthermore, we enlisted 13 male volunteers to consume 35 g delipidated cocoa. Venous blood samples were taken before and at 2 h and 4 h after consuming the cocoa. The oxidation lag time of LDL before cocoa ingestion was 59.0 +/- 6.3 min, but it was prolonged at 2 h after cocoa (68.3 +/- 6.0 min); before returning to the initial lag time (61.7 +/- 5.7 min) before consumption. Thus we have shown that cocoa inhibited LDL oxidation both in vitro and ex vivo.


Subject(s)
Antioxidants/pharmacology , Arteriosclerosis/prevention & control , Cacao/chemistry , Cholesterol, LDL/drug effects , Flavonoids , Phenols/pharmacology , Polymers/pharmacology , Adult , Antioxidants/therapeutic use , Arteriosclerosis/blood , Azo Compounds/pharmacology , Cholesterol, LDL/blood , Cholesterol, LDL/metabolism , Dose-Response Relationship, Drug , Humans , In Vitro Techniques , Male , Nitriles/pharmacology , Oxidation-Reduction , Phenols/therapeutic use , Polymers/therapeutic use , Polyphenols , Spectrophotometry , Time Factors
11.
Biosci Biotechnol Biochem ; 64(12): 2581-7, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11210120

ABSTRACT

The antioxidant polyphenols in cacao liquor, a major ingredient of chocolate and cocoa, have been characterized as flavan-3-ols and proanthocyanidin oligomers. In this study, various cacao products were analyzed by normal-phase HPLC, and the profiles and quantities of the polyphenols present, grouped by molecular size (monomers to approximately oligomers), were compared. Individual cacao polyphenols, flavan-3-ols (catechin and epicatechin), and dimeric (procyanidin B2), trimeric (procyanidin C1), and tetrameric (cinnamtannin A2) proanthocyanidins, and galactopyranosyl-ent-(-)-epicatechin (2alpha-->7, 4alpha-->8)-(-)-epicatechin (Gal-EC-EC), were analyzed by reversed-phase HPLC and/or HPLC/MS. The profile of monomers (catechins) and proanthocyanidin in dark chocolate was similar to that of cacao liquor, while the ratio of flavan-3-ols to the total amount of monomeric and oligomeric polyphenols in the case of pure cocoa powder was higher than that in the case of cacao liquor or chocolate.


Subject(s)
Biflavonoids , Cacao/chemistry , Chromatography, High Pressure Liquid/methods , Flavonoids , Phenols/analysis , Polymers/analysis , Proanthocyanidins , Anthocyanins/analysis , Catechin/analogs & derivatives , Catechin/analysis , Chromatography, High Pressure Liquid/standards , Chromatography, Liquid , Food Handling/methods , Galactose/analogs & derivatives , Galactose/analysis , Mass Spectrometry/methods , Reproducibility of Results
12.
J Electron Microsc (Tokyo) ; 49(6): 711-8, 2000.
Article in English | MEDLINE | ID: mdl-11270851

ABSTRACT

We developed a 1 MV field-emission transmission electron microscope. This paper reports details and specifications of the instrument. The microscope was designed to obtain a bright and coherent electron beam by using the field emission gun equipped with a pre-accelerating magnetic lens and the high-voltage power supply with high stability (0.5 ppm min(-1)). Using this microscope, the brightness of 1.8 x 10(10) A cm(-2) sr(-1) and the lattice resolution of 49.8 pm were attained.

13.
Free Radic Res ; 33(5): 635-41, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11200094

ABSTRACT

We evaluated the levels of (-)-epicatechin (EC) and its metabolites in plasma and urine after intake of chocolate or cocoa by male volunteers. EC metabolites were analyzed by HPLC and LC/MS after glucuronidase and/or sulfatase treatment. The maximum levels of total EC metabolites in plasma were reached 2 hours after either chocolate or cocoa intake. Sulfate, glucuronide, and sulfoglucuronide (mixture of sulfate and glucuronide) conjugates of nonmethylated EC were the main metabolites present in plasma rather than methylated forms. Urinary excretion of total EC metabolites within 24 hours after chocolate or cocoa intake was 29.8+/-5.3%'; and 25.3+/-8.1% of total EC intake. EC in chocolate and cocoa was partly absorbed and was found to be present as a component of various conjugates in plasma, and these were rapidly excreted in urine.


Subject(s)
Cacao , Catechin/pharmacokinetics , Adult , Biological Availability , Catechin/blood , Catechin/urine , Chromatography, High Pressure Liquid , Chromatography, Liquid , Humans , Intestinal Absorption , Male , Mass Spectrometry , Methylation
14.
J Atheroscler Thromb ; 7(3): 164-8, 2000.
Article in English | MEDLINE | ID: mdl-11480458

ABSTRACT

The effects of cacao liquor polyphenols (CLP) on the susceptibility of low-density lipoprotein (LDL) to oxidation in hypercholesterolemic rabbits were examined. Six Japanese white rabbits which had been fed a high cholesterol diet (HCD) for 3 weeks were fed HCD containing 1% CLP for the following 10 days. The susceptibility of LDL to oxidation induced by 2-2'-azobis(4-methoxy-2, 4-dimethylvaleronitrile) (V-70) was evaluated by measuring the production of conjugated dienes and thiobarbituric acid reactive substances (TBARS). The lag time was significantly prolonged from 37.7 min before intake of CLP to 42.9, 44.2 and 45.8 min after 4, 7 and 10 days of CLP intake. TBARS production after intake of CLP was also markedly reduced compared with the level before intake. There was no difference in plasma lipid concentrations comparing the levels before and after CLP intake. In conclusion, in hypercholesterolemic rabbits, orally administered CLP was absorbed and distributed to the blood, and the resistance of LDL to oxidation was thereby increased.


Subject(s)
Antioxidants/pharmacology , Cacao/chemistry , Flavonoids , Hypercholesterolemia/drug therapy , Hypercholesterolemia/metabolism , Lipoproteins, LDL/blood , Phenols/pharmacology , Polymers/pharmacology , Animals , Antioxidants/isolation & purification , Lipids/blood , Lipoproteins, LDL/chemistry , Male , Oxidation-Reduction , Phenols/isolation & purification , Polymers/isolation & purification , Polyphenols , Rabbits , Thiobarbituric Acid Reactive Substances/metabolism
15.
Biosci Biotechnol Biochem ; 62(8): 1535-8, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9757560

ABSTRACT

The antiulcer activity of cacao liquor water-soluble crude polyphenols (CWSP) was examined. CWSP, alpha-tocopherol, sucralfate (500 mg/kg), and cimetidine (250 mg/kg) were orally administered to male SD rats 30 minutes before ethanol treatment. 5 ml/kg of ethanol given intragastrically caused lesions in mucosa of the glandular stomach. CWSP caused a reduction of such hemorrhagic lesions as well as cimetidine and sucralfate which are typical antiulcer drugs, but alpha-tocopherol was less effective. Thiobarbituric acid reactive substances in gastric mucosa significantly increased with ethanol administration. CWSP treatment significantly reduced this change. The administration of ethanol extensively increased myeloperoxidase (MPO) but not xanthine oxidase (XOD) activity. CWSP reduced the activities of both enzymes; they were considered the main sources of oxygen radicals. According to an in vitro study, CWSP directly reducted XOD but not MPO. These results suggest that the antiulcer mechanism of CWSP was not only radical scavenging but also modulation of leukocyte function.


Subject(s)
Antioxidants/pharmacology , Cacao/metabolism , Ethanol/adverse effects , Flavonoids , Gastric Mucosa/drug effects , Phenols/pharmacology , Polymers/pharmacology , Animals , Anti-Ulcer Agents/pharmacology , Anti-Ulcer Agents/therapeutic use , Antioxidants/metabolism , Chromatography, High Pressure Liquid , Cimetidine/pharmacology , Cimetidine/therapeutic use , Dose-Response Relationship, Drug , Gastric Mucosa/pathology , Lipid Peroxidation , Male , Peroxidase/antagonists & inhibitors , Peroxidase/metabolism , Phenols/therapeutic use , Polymers/therapeutic use , Polyphenols , Rats , Rats, Sprague-Dawley , Stomach Ulcer/drug therapy , Sucralfate/pharmacology , Sucralfate/therapeutic use , Thiobarbituric Acid Reactive Substances/analysis , Uric Acid/analysis , Vitamin E/pharmacology , Vitamin E/therapeutic use , Xanthine Oxidase/antagonists & inhibitors , Xanthine Oxidase/metabolism
16.
J Nutr Sci Vitaminol (Tokyo) ; 44(2): 313-21, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9675711

ABSTRACT

The antioxidative substances contained in cacao liquor, which is one of the major ingredients of chocolate, were separated by column chromatography and high-performance liquid chromatography. Three major compounds were purified and two of them were identified by 1H, 13C NMR and mass spectra as (-)-epicatechin (EC) and (+)-catechin (CA). Their antioxidative activity was measured by monitoring the peroxide value of linoleic acid and the thiobarbituric acid-reactive substance values of erythrocyte ghost membranes and microsomes. EC and CA had strong antioxidative effects in all three methods, but one unidentified peak was found to be less effective. Additionally, we analyzed the polyphenol concentration of cacao liquor extractions produced in several countries. The total polyphenol concentration was 7.0 to 13.0%, catechin concentration was 0.31 to 0.49%, and epicatechin concentration was 0.35 to 1.68% in the extractions. It is believed that chocolate is stable against oxidative deterioration on account of the presence of these polyphenolic compounds, and it is also expected to have a protective role against lipid peroxidation in living systems.


Subject(s)
Alcoholic Beverages/analysis , Antioxidants/isolation & purification , Cacao , Flavonoids , Animals , Antioxidants/pharmacology , Catechin/chemistry , Catechin/isolation & purification , Catechin/pharmacology , Chromatography, High Pressure Liquid , Erythrocyte Membrane/metabolism , Linoleic Acid/metabolism , Lipid Peroxidation/drug effects , Magnetic Resonance Spectroscopy , Mass Spectrometry , Microsomes, Liver/metabolism , Oxidation-Reduction , Phenols/analysis , Polymers/analysis , Rats , Thiobarbituric Acid Reactive Substances/metabolism
17.
J Agric Food Chem ; 46(2): 454-457, 1998 Feb 16.
Article in English | MEDLINE | ID: mdl-10554262

ABSTRACT

The antioxidant components of cacao liquor, which is a major ingredient of chocolate, were isolated with column chromatography and high-performance liquid chromatography. Quercetin and its glucoside were identified by spectrometric methods. Clovamide and deoxyclovamide were characterized by (1)H and (13)C NMR and MS spectrometry. Their antioxidative activity was measured by peroxide value of linoleic acid and thiobarbituric acid reactive-substance value of erythrocyte ghost membranes and microsomes. In the bulk oil system, clovamide had the strongest antioxidative activity but was less active in the other experiments. In the case of the two hydrophilic systems, flavans such as quercetin and epicatechin were more potently effective than the glucosides. It is considered that chocolate is stable against oxidative deterioration due to the presence of these polyphenolic compounds.

18.
Microsc Res Tech ; 20(4): 457-62, 1992 Feb 15.
Article in English | MEDLINE | ID: mdl-1498359

ABSTRACT

Reflection electron holography is described as a method to observe sub-A surface morphology. Phase shift of a Bragg-reflected electron wave was measured by means of holographic interferometry using an electron microscope equipped with a field emission electron gun and an electron biprism. A short wavelength of high energy electrons is the essential key to the high vertical sensitivity of this method, since geometrical path differences produced by the surface topography are measured in units of wavelengths in interferometrical measuring. Phase shift at a monoatomic step and the displacement field around a dislocation emerging on the surface were observed.


Subject(s)
Holography/methods , Surface Properties , Electrons , Holography/instrumentation , Microscopy, Electron , Platinum/chemistry , Research Design , Scattering, Radiation
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